Restriction map for pFN21AE3161
 (Circular) (Six-base) MAPSORT of: pFN21AE3161.seq  Check: 9563  from: 1  to: 5830



pFN21AE3161  5830 bp

 With 182 enzymes: SgfI * 

                        December 28, 2011 15:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3640     278 
   Size:         53      83     186     936    2104    2468

  Fragments arranged by size: 

                2468    2104     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2724    3049    3173    1937 
   Size:        787     325     124    4594

  Fragments arranged by size: 

                4594     787     325     124
 
Acc65I G'GTAC_C

Cuts at:   2954    3151    2954 
   Size:        197    5633
 
AclI AA'CG_TT

Cuts at:   1563    3957    4330    1563 
   Size:       2394     373    3063

  Fragments arranged by size: 

                3063    2394     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2154    2168    2386    3893    5277    5545    1632 
   Size:        522      14     218    1507    1384     268    1917

  Fragments arranged by size: 

                1917    1507    1384     522     268     218      14
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5633
 
AflIII A'CryG_T

Cuts at:   1203    3127    4729    5706    1203 
   Size:       1924    1602     977    1327

  Fragments arranged by size: 

                1924    1602    1327     977
 
AgeI A'CCGG_T

Cuts at:   2061    4661    2061 
   Size:       2600    3230
 
AhdI GACnn_n'nnGTC

Cuts at:   4559    4559 
   Size:       5830
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5830
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5830
 
AlwNI CAG_nnn'CTG

Cuts at:   3573    5145    3573 
   Size:       1572    4258
 
ApaLI G'TGCA_C

Cuts at:   3887    5043    3887 
   Size:       1156    4674
 
ApoI r'AATT_y

Cuts at:   1493    2851    2928    3139    3365    3517    5626    1493 
   Size:       1358      77     211     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1358     226     211     152      77
 
AseI AT'TA_AT

Cuts at:    160    1032    4384     160 
   Size:        872    3352    1606

  Fragments arranged by size: 

                3352    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5830
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3155    1870 
   Size:         73    1212    4545

  Fragments arranged by size: 

                4545    1212      73
 
BamHI G'GATC_C

Cuts at:   2827    3160    3527    2827 
   Size:        333     367    5130

  Fragments arranged by size: 

                5130     367     333
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2954    3151    4606     618 
   Size:        325     217     627    1167     197    1455    1842

  Fragments arranged by size: 

                1842    1455    1167     627     325     217     197
 
BanII G_rGCy'C

Cuts at:   1391    2549    3149    1391 
   Size:       1158     600    4072

  Fragments arranged by size: 

                4072    1158     600
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2639     928 
   Size:        971     740    4119

  Fragments arranged by size: 

                4119     971     740
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2011    2045    4021    4055    1903 
   Size:         34      74      34    1976      34    3678

  Fragments arranged by size: 

                3678    1976      74      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2192    2386    2487    3722    4932    2192 
   Size:        194     101    1235    1210    3090

  Fragments arranged by size: 

                3090    1235    1210     194     101
 
BclI T'GATC_A

Cuts at:   1548    2965    1548 
   Size:       1417    4413
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4441     136 
   Size:        107     122      71     631     605    2769    1525

  Fragments arranged by size: 

                2769    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5825    5825 
   Size:       5830
 
BlpI GC'TnA_GC

Cuts at:   2804    3247    2804 
   Size:        443    5387
 
Bme1580I G_kGCm'C

Cuts at:   1790    2042    3891    5047    1790 
   Size:        252    1849    1156    2573

  Fragments arranged by size: 

                2573    1849    1156     252
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5830
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2323    2980    3561 
   Size:        416      53      83     399      24     906     657     581

Cuts at:   3561    4519     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     906     657     581     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5830
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4490    1239 
   Size:       3251    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2097    2097 
   Size:       5830
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2323    2444    2666    3955    4820    5118    2323 
   Size:        121     222    1289     865     298    3035

  Fragments arranged by size: 

                3035    1289     865     298     222     121
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4493     882 
   Size:        617    2994    2219

  Fragments arranged by size: 

                2994    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3621     493 
   Size:        711    2417    2702

  Fragments arranged by size: 

                2702    2417     711
 
BsaBI GATnn'nnATC

Cuts at:   5824    5824 
   Size:       5830
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3637    4019    4654     275 
   Size:         53      83     186     936    2104     382     635    1451

  Fragments arranged by size: 

                2104    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2061    4263    4661    4935    5082    5493    2061 
   Size:       2202     398     274     147     411    2398

  Fragments arranged by size: 

                2398    2202     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5605

  Fragments arranged by size: 

                5605     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2310    2313    1186 
   Size:       1124       3    4703

  Fragments arranged by size: 

                4703    1124       3
 
BseYI C'CCAG_C

Cuts at:   5033    5529    5033 
   Size:        496    5334
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5257
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2066    3280    4041    5069    1540 
   Size:        275     186      65    1214     761    1028    2301

  Fragments arranged by size: 

                2301    1214    1028     761     275     186      65
 
BsiHKAI G_wGCw'C

Cuts at:   2549    3891    3976    5047    2549 
   Size:       1342      85    1071    3332

  Fragments arranged by size: 

                3332    1342    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2158    2229    2600    3341    3434    2158 
   Size:         71     371     741      93    4554

  Fragments arranged by size: 

                4554     741     371      93      71
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2042    2549    3149    3891    3976    5047    1391 
   Size:        399     252     507     600     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     600     507     399     252      85
            
 
BspEI T'CCGG_A

Cuts at:   5493    5493 
   Size:       5830
 
BspHI T'CATG_A

Cuts at:   3719    5449    3719 
   Size:       1730    4100
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2405    3185     844 
   Size:       1561     780    3489

  Fragments arranged by size: 

                3489    1561     780
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3717    1394 
   Size:        429    1894    3507

  Fragments arranged by size: 

                3507    1894     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4325    4499      65 
   Size:       1183    3077     174    1396

  Fragments arranged by size: 

                3077    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2061    4474    4661    1700 
   Size:        110     251    2413     187    2869

  Fragments arranged by size: 

                2869    2413     251     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5830
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5830
 
BssSI C'ACGA_G

Cuts at:   2886    3890    4902    2886 
   Size:       1004    1012    3814

  Fragments arranged by size: 

                3814    1012    1004
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2429     794 
   Size:       1635    4195
 
BstBI TT'CG_AA

Cuts at:   4634    4634 
   Size:       5830
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5830
 
BstYI r'GATC_y

Cuts at:   1972    2827    3160    3527    3912    3929    5381    5467    5479 
   Size:        855     333     367     385      17    1452      86      12

Cuts at:   5479    5825    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     855     385     367     346     333      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2117    2117 
   Size:       5830
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2663     513 
   Size:        552     939     659    3680

  Fragments arranged by size: 

                3680     939     659     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5091

  Fragments arranged by size: 

                5091     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3346    4140    4160    3346 
   Size:        794      20    5016

  Fragments arranged by size: 

                5016     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2625    1554 
   Size:       1071    4759
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5795
 
DraI TTT'AAA

Cuts at:   3134    3134 
   Size:       5830
 
DraIII CAC_nnn'GTG

Cuts at:   2669    2669 
   Size:       5830
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4837     809 
   Size:        507    3521    1802

  Fragments arranged by size: 

                3521    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3277    4166    5795 
   Size:         54    1425      50     275      18    1447     889    1629

Cuts at:   5795       8 
   Size:         43

  Fragments arranged by size: 

                1629    1447    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3277    1537 
   Size:        275    1465    4090

  Fragments arranged by size: 

                4090    1465     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2098    2346    3760    1353 
   Size:        745     248    1414    3423

  Fragments arranged by size: 

                3423    1414     745     248
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4417    4791    4937     387 
   Size:       1064     138    2828     374     146    1280

  Fragments arranged by size: 

                2828    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2547    2547 
   Size:       5830
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2154    2168    2386    3893    4490    5277    5545 
   Size:        393     522      14     218    1507     597     787     268

Cuts at:   5545    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1507     787     597     522     393     268     218
                  14
 
EcoRI G'AATT_C

Cuts at:   3139    3517    3139 
   Size:        378    5452
 
FspI TGC'GCA

Cuts at:   4336    4336 
   Size:       5830
 
HaeII r_GCGC'y

Cuts at:   1678    4977    1678 
   Size:       3299    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2347    2379    4471    4503    4545 
   Size:         32     135      32     775      32    2092      32      42

Cuts at:   4545    4577    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2092     775     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3174    3420     669 
   Size:        988     281    1236     246    3079

  Fragments arranged by size: 

                3079    1236     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5830
 
HpaI GTT'AAC

Cuts at:   3420    3420 
   Size:       5830
 
KpnI G_GTAC'C

Cuts at:   2958    3155    2958 
   Size:        197    5633
 
MfeI C'AATT_G

Cuts at:   3429    3429 
   Size:       5830
 
MluI A'CGCG_T

Cuts at:   5706    5706 
   Size:       5830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4944    5128    1305 
   Size:       3639     184    2007

  Fragments arranged by size: 

                3639    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4008

  Fragments arranged by size: 

                4008    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2009    3788    4147    4306     518 
   Size:        648     843    1779     359     159    2042

  Fragments arranged by size: 

                2042    1779     843     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2179    3246    3923    5071    5316    1606 
   Size:        573    1067     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1067     677     573     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4339

  Fragments arranged by size: 

                4339     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5830
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5830
 
NotI GC'GGCC_GC

Cuts at:   3277    3277 
   Size:       5830
 
NspI r_CATG'y

Cuts at:   1132    1229    3188    4733    1132 
   Size:         97    1959    1545    2229

  Fragments arranged by size: 

                2229    1959    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2791    1348 
   Size:       1443    4387
 
PciI A'CATG_T

Cuts at:   4729    4729 
   Size:       5830
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5830
 
PfoI T'CCnGG_A

Cuts at:   2531    2895    2531 
   Size:        364    5466
 
PmeI GTTT'AAAC

Cuts at:   3134    3134 
   Size:       5830
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3899    3931    5412    5444    3899 
   Size:         32    1481      32    4285

  Fragments arranged by size: 

                4285    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5830
 
PsiI TTA'TAA

Cuts at:   3400    3400 
   Size:       5830
 
PstI C_TGCA'G

Cuts at:    830    2485    3182     830 
   Size:       1655     697    3478

  Fragments arranged by size: 

                3478    1655     697
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5830
 
PvuII CAG'CTG

Cuts at:   2179    2179 
   Size:       5830
 
SacI G_AGCT'C

Cuts at:   2549    2549 
   Size:       5830
 
SalI G'TCGA_C

Cuts at:   1936    3172    1936 
   Size:       1236    4594
 
SbfI CC_TGCA'GG

Cuts at:   3182    3182 
   Size:       5830
 
ScaI AGT'ACT

Cuts at:   4078    5790    4078 
   Size:       1712    4118
 
SfcI C'TryA_G

Cuts at:    826    1045    2481    3178    4313    4994    5185     826 
   Size:        219    1436     697    1135     681     191    1471

  Fragments arranged by size: 

                1471    1436    1135     697     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5830
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5830
 
SmaI CCC'GGG

Cuts at:   1872    3157    1872 
   Size:       1285    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2338    2459    2645    3934    4835    5097 
   Size:        197     926     395     121     186    1289     901     262

Cuts at:   5097     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1289     926     901     395     262     197     186
                 121
 
SnaBI TAC'GTA

Cuts at:    493    3621     493 
   Size:       3128    2702
 
SpeI A'CTAG_T

Cuts at:    152    3044     152 
   Size:       2892    2938
 
SphI G_CATG'C

Cuts at:   1132    3188    1132 
   Size:       2056    3774
 
SspI AAT'ATT

Cuts at:      5      52    3754       5 
   Size:         47    3702    2081

  Fragments arranged by size: 

                3702    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5830
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3269     513 
   Size:        552     939    1265    3074

  Fragments arranged by size: 

                3074    1265     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3857    3874    4027    4212    4657     325 
   Size:       1255    2277      17     153     185     445    1498

  Fragments arranged by size: 

                2277    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2212    4076    5788 
   Size:        274      80      33      51    1443     235    1864    1712

Cuts at:   5788      96 
   Size:        138

  Fragments arranged by size: 

                1864    1712    1443     274     235     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5790    5822    5790 
   Size:         32    5798
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5830
 
XbaI T'CTAG_A

Cuts at:   3166    3166 
   Size:       5830
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5830
 
XmaI C'CCGG_G

Cuts at:   1870    3155    1870 
   Size:       1285    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3959    3959 
   Size:       5830
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3638     276 
   Size:         53      83     186     936    2104    2468

  Fragments arranged by size: 

                2468    2104     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I    EcoNI
 EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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