(Circular) (Six-base) MAPSORT of: pFN21AE3161.seq Check: 9563 from: 1 to: 5830 pFN21AE3161 5830 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2724 3049 3173 1937 Size: 787 325 124 4594 Fragments arranged by size: 4594 787 325 124 Acc65I G'GTAC_C Cuts at: 2954 3151 2954 Size: 197 5633 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2168 2386 3893 5277 5545 1632 Size: 522 14 218 1507 1384 268 1917 Fragments arranged by size: 1917 1507 1384 522 268 218 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 3127 4729 5706 1203 Size: 1924 1602 977 1327 Fragments arranged by size: 1924 1602 1327 977 AgeI A'CCGG_T Cuts at: 2061 4661 2061 Size: 2600 3230 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2851 2928 3139 3365 3517 5626 1493 Size: 1358 77 211 226 152 2109 1697 Fragments arranged by size: 2109 1697 1358 226 211 152 77 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 2827 3160 3527 2827 Size: 333 367 5130 Fragments arranged by size: 5130 367 333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2954 3151 4606 618 Size: 325 217 627 1167 197 1455 1842 Fragments arranged by size: 1842 1455 1167 627 325 217 197 BanII G_rGCy'C Cuts at: 1391 2549 3149 1391 Size: 1158 600 4072 Fragments arranged by size: 4072 1158 600 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2639 928 Size: 971 740 4119 Fragments arranged by size: 4119 971 740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2011 2045 4021 4055 1903 Size: 34 74 34 1976 34 3678 Fragments arranged by size: 3678 1976 74 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2192 2386 2487 3722 4932 2192 Size: 194 101 1235 1210 3090 Fragments arranged by size: 3090 1235 1210 194 101 BclI T'GATC_A Cuts at: 1548 2965 1548 Size: 1417 4413 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 2804 3247 2804 Size: 443 5387 Bme1580I G_kGCm'C Cuts at: 1790 2042 3891 5047 1790 Size: 252 1849 1156 2573 Fragments arranged by size: 2573 1849 1156 252 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2980 3561 Size: 416 53 83 399 24 906 657 581 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 958 906 657 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4490 1239 Size: 3251 2579 Bpu10I CC'TnA_GC Cuts at: 2097 2097 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2323 2444 2666 3955 4820 5118 2323 Size: 121 222 1289 865 298 3035 Fragments arranged by size: 3035 1289 865 298 222 121 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 4263 4661 4935 5082 5493 2061 Size: 2202 398 274 147 411 2398 Fragments arranged by size: 2398 2202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2310 2313 1186 Size: 1124 3 4703 Fragments arranged by size: 4703 1124 3 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 3280 4041 5069 1540 Size: 275 186 65 1214 761 1028 2301 Fragments arranged by size: 2301 1214 1028 761 275 186 65 BsiHKAI G_wGCw'C Cuts at: 2549 3891 3976 5047 2549 Size: 1342 85 1071 3332 Fragments arranged by size: 3332 1342 1071 85 BsmI GAATG_Cn' Cuts at: 2158 2229 2600 3341 3434 2158 Size: 71 371 741 93 4554 Fragments arranged by size: 4554 741 371 93 71 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2549 3149 3891 3976 5047 1391 Size: 399 252 507 600 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 507 399 252 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2405 3185 844 Size: 1561 780 3489 Fragments arranged by size: 3489 1561 780 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 4474 4661 1700 Size: 110 251 2413 187 2869 Fragments arranged by size: 2869 2413 251 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2886 3890 4902 2886 Size: 1004 1012 3814 Fragments arranged by size: 3814 1012 1004 BstAPI GCAn_nnn'nTGC Cuts at: 794 2429 794 Size: 1635 4195 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2827 3160 3527 3912 3929 5381 5467 5479 Size: 855 333 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 855 385 367 346 333 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2117 2117 Size: 5830 BtgI C'CryG_G Cuts at: 513 1065 2004 2663 513 Size: 552 939 659 3680 Fragments arranged by size: 3680 939 659 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 2625 1554 Size: 1071 4759 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DraIII CAC_nnn'GTG Cuts at: 2669 2669 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795 Size: 54 1425 50 275 18 1447 889 1629 Cuts at: 5795 8 Size: 43 Fragments arranged by size: 1629 1447 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2098 2346 3760 1353 Size: 745 248 1414 3423 Fragments arranged by size: 3423 1414 745 248 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2547 2547 Size: 5830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2168 2386 3893 4490 5277 5545 Size: 393 522 14 218 1507 597 787 268 Cuts at: 5545 1239 Size: 1524 Fragments arranged by size: 1524 1507 787 597 522 393 268 218 14 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 4471 4503 4545 Size: 32 135 32 775 32 2092 32 42 Cuts at: 4545 4577 1373 Size: 32 2626 Fragments arranged by size: 2626 2092 775 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 2958 3155 2958 Size: 197 5633 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3788 4147 4306 518 Size: 648 843 1779 359 159 2042 Fragments arranged by size: 2042 1779 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 3246 3923 5071 5316 1606 Size: 573 1067 677 1148 245 2120 Fragments arranged by size: 2120 1148 1067 677 573 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 2791 1348 Size: 1443 4387 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PfoI T'CCnGG_A Cuts at: 2531 2895 2531 Size: 364 5466 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PstI C_TGCA'G Cuts at: 830 2485 3182 830 Size: 1655 697 3478 Fragments arranged by size: 3478 1655 697 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2179 2179 Size: 5830 SacI G_AGCT'C Cuts at: 2549 2549 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2481 3178 4313 4994 5185 826 Size: 219 1436 697 1135 681 191 1471 Fragments arranged by size: 1471 1436 1135 697 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 2459 2645 3934 4835 5097 Size: 197 926 395 121 186 1289 901 262 Cuts at: 5097 820 Size: 1553 Fragments arranged by size: 1553 1289 926 901 395 262 197 186 121 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 3044 152 Size: 2892 2938 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 3269 513 Size: 552 939 1265 3074 Fragments arranged by size: 3074 1265 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2212 4076 5788 Size: 274 80 33 51 1443 235 1864 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1864 1712 1443 274 235 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI