################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BfaI BfrI BlnI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MspA1I MunI MvnI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 625 GTCGAC GT^[AC][GT]AC AccIII 431 TCCGGA T^CCGGA AflIII 1292 ACATGT A^C[GA][CT]GT AlwNI 679 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 1159 GTGCTC G[AT]GC[AT]^C BanI 1166 GGTGCC G^G[CT][GA]CC BciVI 335 GTATCC GTATCC(6/5) BclI 971 TGATCA T^GATCA BcnI 339 CCCGG CC^[GC]GG BglII 1376 AGATCT A^GATCT BlpI 323 GCTCAGC GC^T[ACGT]AGC Bpu1102I 323 GCTCAGC GC^T[ACGT]AGC BsaI 651 GGTCTC GGTCTC(1/5) BsaWI 431 TCCGGA [AT]^CCGG[AT] BseAI 431 TCCGGA T^CCGGA BsiHKAI 1159 GTGCTC G[AT]GC[AT]^C BspEI 431 TCCGGA T^CCGGA BspLU11I 1292 ACATGT A^CATGT BsrDI 1043 GCAATG GCAATG(2/0) CelII 323 GCTCAGC GC^T[ACGT]AGC DraII 761 GGGTCCC [GA]G^G[ACGT]CC[CT] EarI 271 CTCTTC CTCTTC(1/4) EcoO109I 761 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoT22I 1543 ATGCAT ATGCA^T FbaI 971 TGATCA T^GATCA Ksp632I 271 CTCTTC CTCTTC(1/4) MaeII 845 ACGT A^CGT MroI 431 TCCGGA T^CCGGA MseI 1111 TTAA T^TAA NaeI 804 GCCGGC GCC^GGC NciI 339 CCCGG CC^[GC]GG NcoI 1226 CCATGG C^CATGG NgoMIV 804 GCCGGC G^CCGGC NsiI 1543 ATGCAT ATGCA^T NspI 1292 ACATGT [GA]CATG^[CT] PpuMI 761 GGGTCCC [GA]G^G[AT]CC[CT] SalI 625 GTCGAC G^TCGAC SmlI 698 CTCAAG C^T[CT][GA]AG StuI 1182 AGGCCT AGG^CCT Tru9I 1111 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 148 GCGG CCGC(-3/-1) 687 GCGG AcsI 220 AAATTC [GA]^AATT[CT] 1318 AAATTT ApoI 220 AAATTC [GA]^AATT[CT] 1318 AAATTT AvaII 762 GGTCC G^G[AT]CC 1326 GGACC BglI 526 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 799 GCCATGCCGGC BmyI 711 GTGCCC G[AGT]GC[ACT]^C 1159 GTGCTC BseRI 487 CTCCTC GAGGAG(10/8) 1477 GAGGAG Bsp1286I 711 GTGCCC G[AGT]GC[ACT]^C 1159 GTGCTC BsrFI 425 ACCGGC [GA]^CCGG[CT] 804 GCCGGC Cac8I 804 GCCGGC GC[ACGT]^[ACGT]GC 898 GCAAGC Cfr10I 425 ACCGGC [GA]^CCGG[CT] 804 GCCGGC HincII 625 GTCGAC GT[CT]^[GA]AC 1249 GTCAAC HindII 625 GTCGAC GT[CT]^[GA]AC 1249 GTCAAC PstI 838 CTGCAG CTGCA^G 893 CTGCAG TfiI 946 GATTC G^A[AT]TC 1602 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AfaI AluI AlwI BbsI BbvI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BspHI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cfr13I DdeI DpnII DsaI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII