(Circular) (Six-base) MAPSORT of: pFN21AE9416.seq Check: 1598 from: 1 to: 6325 pFN21AE9416 6325 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 3668 1937 Size: 694 1037 4594 Fragments arranged by size: 4594 1037 694 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2009 2087 2203 2308 2423 3579 4388 5772 Size: 377 78 116 105 115 1156 809 1384 Cuts at: 5772 6040 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1156 809 377 268 116 115 105 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 3297 5224 6201 1203 Size: 2094 1927 977 1327 Fragments arranged by size: 2094 1927 1327 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2129 2312 2972 1166 Size: 963 183 660 4519 Fragments arranged by size: 4519 963 660 183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 2689 4068 5640 2689 Size: 1379 1572 3374 Fragments arranged by size: 3374 1572 1379 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2225 3323 3634 3860 4012 6121 1493 Size: 732 1098 311 226 152 2109 1697 Fragments arranged by size: 2109 1697 1098 732 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3171 3646 5101 618 Size: 325 217 627 1384 475 1455 1842 Fragments arranged by size: 1842 1455 1384 627 475 325 217 BanII G_rGCy'C Cuts at: 1391 3644 1391 Size: 2253 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 2185 2411 2564 2999 928 Size: 971 194 92 226 153 435 4254 Fragments arranged by size: 4254 971 435 226 194 153 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4516 4550 1903 Size: 34 2579 34 3678 Fragments arranged by size: 3678 2579 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2351 4217 5427 2351 Size: 1866 1210 3249 Fragments arranged by size: 3249 1866 1210 BclI T'GATC_A Cuts at: 1548 2976 1548 Size: 1428 4897 BfrBI ATG'CAT Cuts at: 3550 3550 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2537 2810 4936 Size: 107 122 71 631 605 865 273 2126 Cuts at: 4936 136 Size: 1525 Fragments arranged by size: 2126 1525 865 631 605 273 122 107 71 BglII A'GATC_T Cuts at: 3381 6320 3381 Size: 2939 3386 BlpI GC'TnA_GC Cuts at: 2329 3742 2329 Size: 1413 4912 Bme1580I G_kGCm'C Cuts at: 1790 2720 4386 5542 1790 Size: 930 1666 1156 2573 Fragments arranged by size: 2573 1666 1156 930 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3023 4056 5014 Size: 416 53 83 399 24 1606 1033 958 Cuts at: 5014 442 Size: 1753 Fragments arranged by size: 1753 1606 1033 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2897 3028 4985 1239 Size: 836 822 131 1957 2579 Fragments arranged by size: 2579 1957 836 822 131 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2688 4450 5315 5613 2688 Size: 1762 865 298 3400 Fragments arranged by size: 3400 1762 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2662 4988 882 Size: 617 1163 2326 2219 Fragments arranged by size: 2326 2219 1163 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4132 4514 5149 275 Size: 53 83 186 936 2599 382 635 1451 Fragments arranged by size: 2599 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2436 4758 5156 5430 5577 5988 2436 Size: 2322 398 274 147 411 2773 Fragments arranged by size: 2773 2322 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3254 3284 1178 Size: 30 165 30 1851 30 4219 Fragments arranged by size: 4219 1851 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2483 3497 1186 Size: 1297 1014 4014 Fragments arranged by size: 4014 1297 1014 BseYI C'CCAG_C Cuts at: 5528 6024 5528 Size: 496 5829 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2411 2467 2795 3165 3596 1163 Size: 573 675 56 328 370 431 3892 Fragments arranged by size: 3892 675 573 431 370 328 56 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3168 4386 4471 5542 3168 Size: 1218 85 1071 3951 Fragments arranged by size: 3951 1218 1071 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2720 3168 3644 4386 4471 5542 1391 Size: 399 930 448 476 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 476 448 399 85 BspEI T'CCGG_A Cuts at: 2436 5988 2436 Size: 3552 2773 BspHI T'CATG_A Cuts at: 2247 2673 3277 4214 5944 2247 Size: 426 604 937 1730 2628 Fragments arranged by size: 2628 1730 937 604 426 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 844 Size: 2836 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3055 4820 4994 65 Size: 1183 1807 1765 174 1396 Fragments arranged by size: 1807 1765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 2809 4969 5156 1700 Size: 110 620 379 2160 187 2869 Fragments arranged by size: 2869 2160 620 379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 2131 2890 2965 1184 Size: 947 759 75 4544 Fragments arranged by size: 4544 947 759 75 BstXI CCAn_nnnn'nTGG Cuts at: 2887 2962 3341 2887 Size: 75 379 5871 Fragments arranged by size: 5871 379 75 BstYI r'GATC_y Cuts at: 1972 2107 2788 3381 3655 4022 4407 4424 5876 Size: 135 681 593 274 367 385 17 1452 Cuts at: 5876 5962 5974 6320 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 681 593 385 367 346 274 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2860 2961 3231 513 Size: 552 1795 101 270 3607 Fragments arranged by size: 3607 1795 552 270 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 2421 2940 3841 4635 4655 2421 Size: 519 901 794 20 4091 Fragments arranged by size: 4091 901 794 519 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290 Size: 54 1425 50 275 18 1942 889 1629 Cuts at: 6290 8 Size: 43 Fragments arranged by size: 1942 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 4255 1353 Size: 929 1973 3423 Fragments arranged by size: 3423 1973 929 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2009 2075 2087 2203 2308 2423 2897 Size: 393 377 66 12 116 105 115 474 Cuts at: 2897 3028 3579 4388 4985 5772 6040 1239 Size: 131 551 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 597 551 474 393 377 268 131 116 115 105 66 12 EcoO109I rG'GnC_Cy Cuts at: 2767 2767 Size: 6325 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 5472 1678 Size: 3794 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2778 2810 2901 2933 4966 Size: 32 135 32 1206 32 91 32 2033 Cuts at: 4966 4998 5040 5072 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2033 1206 135 91 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2632 3256 3669 3915 669 Size: 988 281 694 624 413 246 3079 Fragments arranged by size: 3079 988 694 624 413 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5439 5623 1305 Size: 4134 184 2007 Fragments arranged by size: 4134 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2312 2393 2972 4283 4642 4801 Size: 648 963 183 81 579 1311 359 159 Cuts at: 4801 518 Size: 2042 Fragments arranged by size: 2042 1311 963 648 579 359 183 159 81 MspA1I CmG'CkG Cuts at: 1606 3741 4418 5566 5811 1606 Size: 2135 677 1148 245 2120 Fragments arranged by size: 2135 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2811 2811 Size: 6325 NcoI C'CATG_G Cuts at: 513 1065 3231 513 Size: 552 2166 3607 Fragments arranged by size: 3607 2166 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6325 NgoMIV G'CCGG_C Cuts at: 2809 2809 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NsiI A_TGCA'T Cuts at: 3552 3552 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 3301 3683 5228 1132 Size: 97 2072 382 1545 2229 Fragments arranged by size: 2229 2072 1545 382 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 3297 5224 3297 Size: 1927 4398 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PfoI T'CCnGG_A Cuts at: 2091 2343 2091 Size: 252 6073 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2767 2767 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2816 2848 3021 3053 2816 Size: 32 173 32 6088 Fragments arranged by size: 6088 173 32 32 PstI C_TGCA'G Cuts at: 830 2847 2902 3677 830 Size: 2017 55 775 3478 Fragments arranged by size: 3478 2017 775 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 SalI G'TCGA_C Cuts at: 1936 2630 3667 1936 Size: 694 1037 4594 Fragments arranged by size: 4594 1037 694 SanDI GG'GwC_CC Cuts at: 2767 2767 Size: 6325 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2843 2898 3451 3673 4808 5489 5680 Size: 219 1798 55 553 222 1135 681 191 Cuts at: 5680 826 Size: 1471 Fragments arranged by size: 1798 1471 1135 681 553 222 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2703 4429 5330 5592 820 Size: 197 926 760 1726 901 262 1553 Fragments arranged by size: 1726 1553 926 901 760 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 3189 1517 Size: 1672 4653 StyI C'CwwG_G Cuts at: 513 1065 2127 2535 2946 3024 3231 3764 513 Size: 552 1062 408 411 78 207 533 3074 Fragments arranged by size: 3074 1062 552 533 411 408 207 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4352 4369 4522 4707 5152 325 Size: 1255 2772 17 153 185 445 1498 Fragments arranged by size: 2772 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2138 4571 6283 Size: 274 80 33 51 1443 161 2433 1712 Cuts at: 6283 96 Size: 138 Fragments arranged by size: 2433 1712 1443 274 161 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI