(Linear) (Six-base) MAPSORT of: KIEE6744.seq Check: 7474 from: 1 to: 2919 KIEE6744.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 257 785 2919 Size: 257 528 2134 Fragments arranged by size: 2134 528 257 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1009 1214 1314 1400 1496 1526 1965 2919 Size: 1009 205 100 86 96 30 439 954 Fragments arranged by size: 1009 954 439 205 100 96 86 30 AflII C'TTAA_G Cuts at: 0 997 2919 Size: 997 1922 AflIII A'CryG_T Cuts at: 0 260 2919 Size: 260 2659 AleI CACnn'nnGTG Cuts at: 0 511 1195 2919 Size: 511 684 1724 Fragments arranged by size: 1724 684 511 AlwNI CAG_nnn'CTG Cuts at: 0 1254 2061 2919 Size: 1254 807 858 Fragments arranged by size: 1254 858 807 ApaI G_GGCC'C Cuts at: 0 2819 2919 Size: 2819 100 ApoI r'AATT_y Cuts at: 0 903 1372 1467 1786 2161 2919 Size: 903 469 95 319 375 758 Fragments arranged by size: 903 758 469 375 319 95 AseI AT'TA_AT Cuts at: 0 1033 2919 Size: 1033 1886 AvaI C'yCGr_G Cuts at: 0 1697 2919 Size: 1697 1222 BanI G'GyrC_C Cuts at: 0 567 2105 2919 Size: 567 1538 814 Fragments arranged by size: 1538 814 567 BanII G_rGCy'C Cuts at: 0 1698 1784 1886 2819 2919 Size: 1698 86 102 933 100 Fragments arranged by size: 1698 933 102 100 86 BbeI G_GCGC'C Cuts at: 0 2109 2919 Size: 2109 810 BbsI GAAGACnn'nnnn_ Cuts at: 0 600 1971 2200 2625 2919 Size: 600 1371 229 425 294 Fragments arranged by size: 1371 600 425 294 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1720 1754 2919 Size: 1720 34 1165 Fragments arranged by size: 1720 1165 34 BclI T'GATC_A Cuts at: 0 2141 2919 Size: 2141 778 BglII A'GATC_T Cuts at: 0 1223 2919 Size: 1223 1696 Bme1580I G_kGCm'C Cuts at: 0 2819 2919 Size: 2819 100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 949 1139 1276 1712 2250 2911 2919 Size: 648 301 190 137 436 538 661 8 Fragments arranged by size: 661 648 538 436 301 190 137 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 1891 2919 Size: 308 1583 1028 Fragments arranged by size: 1583 1028 308 BsaI GGTCTCn'nnnn_ Cuts at: 0 355 485 2450 2919 Size: 355 130 1965 469 Fragments arranged by size: 1965 469 355 130 BsaAI yAC'GTr Cuts at: 0 2328 2919 Size: 2328 591 BsaHI Gr'CG_yC Cuts at: 0 2106 2591 2919 Size: 2106 485 328 Fragments arranged by size: 2106 485 328 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1471 1855 2095 2824 2874 2919 Size: 1471 384 240 729 50 45 Fragments arranged by size: 1471 729 384 240 50 45 BseYI C'CCAG_C Cuts at: 0 1357 2266 2399 2808 2919 Size: 1357 909 133 409 111 Fragments arranged by size: 1357 909 409 133 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2463 2919 Size: 2463 456 BsiHKAI G_wGCw'C Cuts at: 0 921 1698 1784 2126 2858 2919 Size: 921 777 86 342 732 61 Fragments arranged by size: 921 777 732 342 86 61 BsmI GAATG_Cn' Cuts at: 0 526 1094 2919 Size: 526 568 1825 Fragments arranged by size: 1825 568 526 Bsp1286I G_dGCh'C Cuts at: 0 921 1698 1784 1886 2126 2819 2858 2919 Size: 921 777 86 102 240 693 39 61 Fragments arranged by size: 921 777 693 240 102 86 61 39 BspHI T'CATG_A Cuts at: 0 1112 2426 2919 Size: 1112 1314 493 Fragments arranged by size: 1314 1112 493 BsrDI GCAATG_nn' Cuts at: 0 1494 2030 2090 2919 Size: 1494 536 60 829 Fragments arranged by size: 1494 829 536 60 BsrGI T'GTAC_A Cuts at: 0 2 2870 2919 Size: 2 2868 49 Fragments arranged by size: 2868 49 2 BssSI C'ACGA_G Cuts at: 0 1180 2919 Size: 1180 1739 BstXI CCAn_nnnn'nTGG Cuts at: 0 2562 2919 Size: 2562 357 BstYI r'GATC_y Cuts at: 0 383 427 1223 1564 2919 Size: 383 44 796 341 1355 Fragments arranged by size: 1355 796 383 341 44 BtgI C'CryG_G Cuts at: 0 251 2919 Size: 251 2668 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2646 2919 Size: 2646 273 BtsI GCAGTG_nn' Cuts at: 0 482 1574 2919 Size: 482 1092 1345 Fragments arranged by size: 1345 1092 482 DraI TTT'AAA Cuts at: 0 1136 2919 Size: 1136 1783 DraIII CAC_nnn'GTG Cuts at: 0 556 2919 Size: 556 2363 EaeI y'GGCC_r Cuts at: 0 248 471 1968 2919 Size: 248 223 1497 951 Fragments arranged by size: 1497 951 248 223 EarI CTCTTCn'nnn_ Cuts at: 0 2072 2294 2743 2919 Size: 2072 222 449 176 Fragments arranged by size: 2072 449 222 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2799 2919 Size: 2799 120 EcoICRI GAG'CTC Cuts at: 0 1696 1782 2919 Size: 1696 86 1137 Fragments arranged by size: 1696 1137 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 949 1009 1139 1214 1276 1314 1400 Size: 648 301 60 130 75 62 38 86 Cuts at: 1400 1496 1526 1712 1965 2250 2911 2919 Size: 96 30 186 253 285 661 8 Fragments arranged by size: 661 648 301 285 253 186 130 96 86 75 62 60 38 30 8 EcoO109I rG'GnC_Cy Cuts at: 0 2358 2816 2919 Size: 2358 458 103 Fragments arranged by size: 2358 458 103 EcoRI G'AATT_C Cuts at: 0 1786 2161 2919 Size: 1786 375 758 Fragments arranged by size: 1786 758 375 EcoRV GAT'ATC Cuts at: 0 1449 2919 Size: 1449 1470 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 724 1245 1881 2919 Size: 724 521 636 1038 Fragments arranged by size: 1038 724 636 521 HaeII r_GCGC'y Cuts at: 0 2109 2919 Size: 2109 810 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 263 295 832 864 1418 1450 2919 Size: 263 32 537 32 554 32 1469 Fragments arranged by size: 1469 554 537 263 32 32 32 HincII GTy'rAC Cuts at: 0 1188 1326 2919 Size: 1188 138 1593 Fragments arranged by size: 1593 1188 138 HindIII A'AGCT_T Cuts at: 0 1508 2919 Size: 1508 1411 KasI G'GCGC_C Cuts at: 0 2105 2919 Size: 2105 814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2114 2919 Size: 2114 805 MscI TGG'CCA Cuts at: 0 250 473 1970 2919 Size: 250 223 1497 949 Fragments arranged by size: 1497 949 250 223 MslI CAynn'nnrTG Cuts at: 0 511 602 1195 1248 1646 1669 2425 2761 Size: 511 91 593 53 398 23 756 336 Cuts at: 2761 2919 Size: 158 Fragments arranged by size: 756 593 511 398 336 158 91 53 23 MspA1I CmG'CkG Cuts at: 0 1150 2228 2919 Size: 1150 1078 691 Fragments arranged by size: 1150 1078 691 NarI GG'CG_CC Cuts at: 0 2106 2919 Size: 2106 813 NcoI C'CATG_G Cuts at: 0 251 2919 Size: 251 2668 NspI r_CATG'y Cuts at: 0 12 264 1147 1179 2919 Size: 12 252 883 32 1740 Fragments arranged by size: 1740 883 252 32 12 PasI CC'CwG_GG Cuts at: 0 423 2919 Size: 423 2496 PciI A'CATG_T Cuts at: 0 260 2919 Size: 260 2659 PpuMI rG'GwC_Cy Cuts at: 0 2358 2919 Size: 2358 561 PshAI GACnn'nnGTC Cuts at: 0 497 2509 2919 Size: 497 2012 410 Fragments arranged by size: 2012 497 410 PspOMI G'GGCC_C Cuts at: 0 2815 2919 Size: 2815 104 PspXI vC'TCGA_Gb Cuts at: 0 1697 2919 Size: 1697 1222 PstI C_TGCA'G Cuts at: 0 489 2511 2919 Size: 489 2022 408 Fragments arranged by size: 2022 489 408 PvuII CAG'CTG Cuts at: 0 1150 2228 2919 Size: 1150 1078 691 Fragments arranged by size: 1150 1078 691 SacI G_AGCT'C Cuts at: 0 1698 1784 2919 Size: 1698 86 1135 Fragments arranged by size: 1698 1135 86 ScaI AGT'ACT Cuts at: 0 2172 2919 Size: 2172 747 SfcI C'TryA_G Cuts at: 0 305 485 2507 2919 Size: 305 180 2022 412 Fragments arranged by size: 2022 412 305 180 SfoI GGC'GCC Cuts at: 0 2107 2919 Size: 2107 812 SmlI C'TyrA_G Cuts at: 0 323 997 1697 1870 2919 Size: 323 674 700 173 1049 Fragments arranged by size: 1049 700 674 323 173 SphI G_CATG'C Cuts at: 0 1147 1179 2919 Size: 1147 32 1740 Fragments arranged by size: 1740 1147 32 StuI AGG'CCT Cuts at: 0 2896 2919 Size: 2896 23 StyI C'CwwG_G Cuts at: 0 251 2919 Size: 251 2668 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 45 487 2587 2800 2919 Size: 45 442 2100 213 119 Fragments arranged by size: 2100 442 213 119 45 TatI w'GTAC_w Cuts at: 0 2 145 2170 2870 2919 Size: 2 143 2025 700 49 Fragments arranged by size: 2025 700 143 49 2 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1376 1408 2522 2554 2633 2665 2919 Size: 1376 32 1114 32 79 32 254 Fragments arranged by size: 1376 1114 254 79 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 39 2683 2919 Size: 39 2644 236 Fragments arranged by size: 2644 236 39 XhoI C'TCGA_G Cuts at: 0 1697 2919 Size: 1697 1222 XmnI GAAnn'nnTTC Cuts at: 0 2632 2919 Size: 2632 287 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlwNI ApaI ApoI AseI AvaI BanI BanII BbeI BbsI BcgI BclI BglII Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PpuMI PshAI PspOMI PspXI PstI PvuII SacI ScaI SfcI SfoI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DrdI EagI EcoNI FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI ZraI