(Circular) (Six-base) MAPSORT of: pFN21AB9897.seq Check: 8869 from: 1 to: 6235 pFN21AB9897 6235 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 3578 1937 Size: 837 804 4594 Fragments arranged by size: 4594 837 804 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2477 2637 4298 5682 5950 1632 Size: 633 212 160 1661 1384 268 1917 Fragments arranged by size: 1917 1661 1384 633 268 212 160 AflII C'TTAA_G Cuts at: 820 1017 3125 820 Size: 197 2108 3930 Fragments arranged by size: 3930 2108 197 AflIII A'CryG_T Cuts at: 1203 2558 3530 5134 6111 1203 Size: 1355 972 1604 977 1327 Fragments arranged by size: 1604 1355 1327 977 972 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 2533 4964 2533 Size: 2431 3804 AleI CACnn'nnGTG Cuts at: 1166 3531 1166 Size: 2365 3870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 3978 5550 3978 Size: 1572 4663 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 2156 2275 2650 2981 3544 3770 3922 6031 Size: 663 119 375 331 563 226 152 2109 Cuts at: 6031 1493 Size: 1697 Fragments arranged by size: 2109 1697 663 563 375 331 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 3110 4789 160 Size: 872 2078 1679 1606 Fragments arranged by size: 2078 1679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 3560 1870 Size: 73 1617 4545 Fragments arranged by size: 4545 1617 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 3556 5011 618 Size: 325 217 627 947 822 1455 1842 Fragments arranged by size: 1842 1455 947 822 627 325 217 BanII G_rGCy'C Cuts at: 1391 3251 3554 1391 Size: 1860 303 4072 Fragments arranged by size: 4072 1860 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 2827 1548 Size: 1279 4956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2726 3121 6230 2726 Size: 395 3109 2731 Fragments arranged by size: 3109 2731 395 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 4296 5452 1790 Size: 2506 1156 2573 Fragments arranged by size: 2573 2506 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3966 4924 442 Size: 416 53 83 399 24 2549 958 1753 Fragments arranged by size: 2549 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4895 1239 Size: 3656 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2924 4360 5225 5523 2924 Size: 1436 865 298 3636 Fragments arranged by size: 3636 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 2879 4898 882 Size: 617 1203 177 2019 2219 Fragments arranged by size: 2219 2019 1203 617 177 BsaAI yAC'GTr Cuts at: 493 1204 2559 4026 493 Size: 711 1355 1467 2702 Fragments arranged by size: 2702 1467 1355 711 BsaBI GATnn'nnATC Cuts at: 2877 2919 6229 2877 Size: 42 3310 2883 Fragments arranged by size: 3310 2883 42 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4042 4424 5059 275 Size: 53 83 186 936 2509 382 635 1451 Fragments arranged by size: 2509 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4668 5066 5340 5487 5898 4668 Size: 398 274 147 411 5005 Fragments arranged by size: 5005 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6010 Fragments arranged by size: 6010 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6235 BseYI C'CCAG_C Cuts at: 5438 5934 5438 Size: 496 5739 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5662 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4296 4381 5452 4296 Size: 85 1071 5079 Fragments arranged by size: 5079 1071 85 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3251 3554 4296 4381 5452 1391 Size: 399 1461 303 742 85 1071 2174 Fragments arranged by size: 2174 1461 1071 742 399 303 85 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2015 3590 844 Size: 1171 1575 3489 Fragments arranged by size: 3489 1575 1171 BsrBI CCG'CTC Cuts at: 1394 1823 4122 1394 Size: 429 2299 3507 Fragments arranged by size: 3507 2299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4879 5066 1700 Size: 110 3069 187 2869 Fragments arranged by size: 3069 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 2198 794 Size: 1404 4831 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2726 3121 3565 3932 4317 4334 5786 5872 Size: 754 395 444 367 385 17 1452 86 Cuts at: 5872 5884 6230 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 754 444 395 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2775 2775 Size: 6235 Bsu36I CC'TnA_GG Cuts at: 3450 3450 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2004 2295 513 Size: 552 939 291 4453 Fragments arranged by size: 4453 939 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3339 3751 4545 4565 2917 Size: 422 412 794 20 4587 Fragments arranged by size: 4587 794 422 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3066 3101 529 Size: 35 2502 35 3663 Fragments arranged by size: 3663 2502 35 35 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 3682 4571 Size: 54 1425 50 275 18 642 1210 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1210 889 642 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2146 3019 3426 4165 1353 Size: 793 873 407 739 3423 Fragments arranged by size: 3423 873 793 739 407 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2810 4822 5196 5342 387 Size: 1064 138 1221 2012 374 146 1280 Fragments arranged by size: 2012 1280 1221 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2477 2637 4298 4895 5682 5950 Size: 393 633 212 160 1661 597 787 268 Cuts at: 5950 1239 Size: 1524 Fragments arranged by size: 1661 1524 787 633 597 393 268 212 160 EcoNI CCTnn'n_nnAGG Cuts at: 2100 2100 Size: 6235 EcoO109I rG'GnC_Cy Cuts at: 2105 2997 3483 2105 Size: 892 486 4857 Fragments arranged by size: 4857 892 486 EcoRI G'AATT_C Cuts at: 2275 2981 3544 3922 2275 Size: 706 563 378 4588 Fragments arranged by size: 4588 706 563 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2595 2595 Size: 6235 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 5382 1678 Size: 3704 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2551 4876 4908 4950 Size: 32 135 32 947 32 2325 32 42 Cuts at: 4950 4982 1373 Size: 32 2626 Fragments arranged by size: 2626 2325 947 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3579 3825 669 Size: 988 281 1641 246 3079 Fragments arranged by size: 3079 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 2468 3834 2468 Size: 1366 4869 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5349 5533 1305 Size: 4044 184 2007 Fragments arranged by size: 4044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 2975 3333 3531 4193 4552 4711 Size: 648 1684 125 358 198 662 359 159 Cuts at: 4711 518 Size: 2042 Fragments arranged by size: 2042 1684 662 648 359 358 198 159 125 MspA1I CmG'CkG Cuts at: 1606 3651 4328 5476 5721 1606 Size: 2045 677 1148 245 2120 Fragments arranged by size: 2120 2045 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2295 513 Size: 552 939 291 4453 Fragments arranged by size: 4453 939 552 291 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3534 3593 5138 1132 Size: 97 2305 59 1545 2229 Fragments arranged by size: 2305 2229 1545 97 59 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6235 PciI A'CATG_T Cuts at: 3530 5134 3530 Size: 1604 4631 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2105 2997 3483 2105 Size: 892 486 4857 Fragments arranged by size: 4857 892 486 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 2141 2403 3805 2141 Size: 262 1402 4571 Fragments arranged by size: 4571 1402 262 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2826 2858 2826 Size: 32 6203 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 2076 4483 6195 2076 Size: 2407 1712 2116 Fragments arranged by size: 2407 2116 1712 SfcI C'TryA_G Cuts at: 826 1045 3425 3583 4718 5399 5590 826 Size: 219 2380 158 1135 681 191 1471 Fragments arranged by size: 2380 1471 1135 681 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 3125 4339 5240 5502 820 Size: 197 926 960 222 1214 901 262 1553 Fragments arranged by size: 1553 1214 960 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 2839 4159 5 Size: 47 2787 1320 2081 Fragments arranged by size: 2787 2081 1320 47 StuI AGG'CCT Cuts at: 1517 2313 1517 Size: 796 5439 StyI C'CwwG_G Cuts at: 513 1065 2004 2295 3000 3674 513 Size: 552 939 291 705 674 3074 Fragments arranged by size: 3074 939 705 674 552 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2752 4262 4279 4432 4617 5062 325 Size: 1255 1172 1510 17 153 185 445 1498 Fragments arranged by size: 1510 1498 1255 1172 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2074 4481 6193 Size: 274 80 33 51 1443 97 2407 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 2407 1712 1443 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3270 3302 6195 6227 3270 Size: 32 2893 32 3278 Fragments arranged by size: 3278 2893 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 2394 3324 4364 2394 Size: 930 1040 4265 Fragments arranged by size: 4265 1040 930 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI