(Circular) (Six-base) MAPSORT of: pFN21AE2332.seq Check: 1376 from: 1 to: 6358 pFN21AE2332 6358 bp With 182 enzymes: SgfI * March 28, 2012 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4168 278 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 3701 1937 Size: 837 927 4594 Fragments arranged by size: 4594 927 837 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2477 2637 4421 5805 6073 1632 Size: 633 212 160 1784 1384 268 1917 Fragments arranged by size: 1917 1784 1384 633 268 212 160 AflII C'TTAA_G Cuts at: 820 1017 3248 820 Size: 197 2231 3930 Fragments arranged by size: 3930 2231 197 AflIII A'CryG_T Cuts at: 1203 2558 2893 3653 5257 6234 1203 Size: 1355 335 760 1604 977 1327 Fragments arranged by size: 1604 1355 1327 977 760 335 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 2533 5087 2533 Size: 2554 3804 AleI CACnn'nnGTG Cuts at: 1166 3654 1166 Size: 2488 3870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6358 AlwNI CAG_nnn'CTG Cuts at: 4101 5673 4101 Size: 1572 4786 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 2156 2275 2650 3104 3667 3893 4045 6154 Size: 663 119 375 454 563 226 152 2109 Cuts at: 6154 1493 Size: 1697 Fragments arranged by size: 2109 1697 663 563 454 375 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 2819 3233 4912 160 Size: 872 1787 414 1679 1606 Fragments arranged by size: 1787 1679 1606 872 414 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 3683 1870 Size: 73 1740 4545 Fragments arranged by size: 4545 1740 73 BamHI G'GATC_C Cuts at: 3688 4055 3688 Size: 367 5991 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2734 3679 5134 618 Size: 325 217 627 947 945 1455 1842 Fragments arranged by size: 1842 1455 947 945 627 325 217 BanII G_rGCy'C Cuts at: 1391 3374 3677 1391 Size: 1983 303 4072 Fragments arranged by size: 4072 1983 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4549 4583 1903 Size: 34 2612 34 3678 Fragments arranged by size: 3678 2612 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 2950 1548 Size: 1402 4956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4969 136 Size: 107 122 71 631 605 3297 1525 Fragments arranged by size: 3297 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2726 3244 6353 2726 Size: 518 3109 2731 Fragments arranged by size: 3109 2731 518 BlpI GC'TnA_GC Cuts at: 3775 3775 Size: 6358 Bme1580I G_kGCm'C Cuts at: 1790 4419 5575 1790 Size: 2629 1156 2573 Fragments arranged by size: 2629 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4089 5047 442 Size: 416 53 83 399 24 2672 958 1753 Fragments arranged by size: 2672 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5018 1239 Size: 3779 2579 Bpu10I CC'TnA_GC Cuts at: 2827 2827 Size: 6358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 4483 5348 5646 3047 Size: 1436 865 298 3759 Fragments arranged by size: 3759 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2702 3002 5021 882 Size: 617 1203 300 2019 2219 Fragments arranged by size: 2219 2019 1203 617 300 BsaAI yAC'GTr Cuts at: 493 1204 2559 4149 493 Size: 711 1355 1590 2702 Fragments arranged by size: 2702 1590 1355 711 BsaBI GATnn'nnATC Cuts at: 3000 3042 6352 3000 Size: 42 3310 3006 Fragments arranged by size: 3310 3006 42 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4165 4547 5182 275 Size: 53 83 186 936 2632 382 635 1451 Fragments arranged by size: 2632 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6133 Fragments arranged by size: 6133 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6358 BseYI C'CCAG_C Cuts at: 5561 6057 5561 Size: 496 5862 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5785 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4419 4504 5575 4419 Size: 85 1071 5202 Fragments arranged by size: 5202 1071 85 BsmI GAATG_Cn' Cuts at: 3869 3962 3869 Size: 93 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3374 3677 4419 4504 5575 1391 Size: 399 1584 303 742 85 1071 2174 Fragments arranged by size: 2174 1584 1071 742 399 303 85 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2015 3713 844 Size: 1171 1698 3489 Fragments arranged by size: 3489 1698 1171 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4853 5027 65 Size: 1183 3605 174 1396 Fragments arranged by size: 3605 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5002 5189 1700 Size: 110 3192 187 2869 Fragments arranged by size: 3192 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 4418 5430 4418 Size: 1012 5346 BstAPI GCAn_nnn'nTGC Cuts at: 794 2198 794 Size: 1404 4954 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2726 3244 3688 4055 4440 4457 5909 5995 Size: 754 518 444 367 385 17 1452 86 Cuts at: 5995 6007 6353 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 754 518 444 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2775 2775 Size: 6358 Bsu36I CC'TnA_GG Cuts at: 3573 3573 Size: 6358 BtgI C'CryG_G Cuts at: 513 1065 2004 2295 513 Size: 552 939 291 4576 Fragments arranged by size: 4576 939 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3462 3874 4668 4688 3040 Size: 422 412 794 20 4710 Fragments arranged by size: 4710 794 422 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3189 3224 529 Size: 35 2625 35 3663 Fragments arranged by size: 3663 2625 35 35 DraI TTT'AAA Cuts at: 2806 3662 2806 Size: 856 5502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2472 2839 3805 Size: 54 1425 50 275 18 642 367 966 Cuts at: 3805 4694 6323 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 966 889 642 367 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2146 3142 3549 4288 1353 Size: 793 996 407 739 3423 Fragments arranged by size: 3423 996 793 739 407 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2933 4945 5319 5465 387 Size: 1064 138 1344 2012 374 146 1280 Fragments arranged by size: 2012 1344 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 2477 2637 4421 5018 5805 6073 Size: 393 633 212 160 1784 597 787 268 Cuts at: 6073 1239 Size: 1524 Fragments arranged by size: 1784 1524 787 633 597 393 268 212 160 EcoNI CCTnn'n_nnAGG Cuts at: 2100 2100 Size: 6358 EcoO109I rG'GnC_Cy Cuts at: 2105 3120 3606 2105 Size: 1015 486 4857 Fragments arranged by size: 4857 1015 486 EcoRI G'AATT_C Cuts at: 2275 3104 3667 4045 2275 Size: 829 563 378 4588 Fragments arranged by size: 4588 829 563 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2595 2595 Size: 6358 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 5505 1678 Size: 3827 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2551 4999 5031 5073 Size: 32 135 32 947 32 2448 32 42 Cuts at: 5073 5105 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 947 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3702 3948 669 Size: 988 281 1764 246 3079 Fragments arranged by size: 3079 1764 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 2468 3957 2468 Size: 1489 4869 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5472 5656 1305 Size: 4167 184 2007 Fragments arranged by size: 4167 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2841 10 Size: 54 1425 343 1009 3527 Fragments arranged by size: 3527 1425 1009 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2973 3098 3456 3654 4316 4675 4834 Size: 648 1807 125 358 198 662 359 159 Cuts at: 4834 518 Size: 2042 Fragments arranged by size: 2042 1807 662 648 359 358 198 159 125 MspA1I CmG'CkG Cuts at: 1606 3774 4451 5599 5844 1606 Size: 2168 677 1148 245 2120 Fragments arranged by size: 2168 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2295 513 Size: 552 939 291 4576 Fragments arranged by size: 4576 939 552 291 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 3657 3716 5261 1132 Size: 97 2428 59 1545 2229 Fragments arranged by size: 2428 2229 1545 97 59 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 3653 5257 3653 Size: 1604 4754 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6358 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2105 3120 3606 2105 Size: 1015 486 4857 Fragments arranged by size: 4857 1015 486 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 2141 2403 3928 2141 Size: 262 1525 4571 Fragments arranged by size: 4571 1525 262 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2949 2981 2949 Size: 32 6326 PstI C_TGCA'G Cuts at: 830 3710 830 Size: 2880 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 2076 4606 6318 2076 Size: 2530 1712 2116 Fragments arranged by size: 2530 2116 1712 SfcI C'TryA_G Cuts at: 826 1045 3548 3706 4841 5522 5713 826 Size: 219 2503 158 1135 681 191 1471 Fragments arranged by size: 2503 1471 1135 681 219 191 158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3026 3248 4462 5363 5625 820 Size: 197 926 1083 222 1214 901 262 1553 Fragments arranged by size: 1553 1214 1083 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 2962 4282 5 Size: 47 2910 1320 2081 Fragments arranged by size: 2910 2081 1320 47 StuI AGG'CCT Cuts at: 1517 2313 1517 Size: 796 5562 StyI C'CwwG_G Cuts at: 513 1065 2004 2295 3123 3797 513 Size: 552 939 291 828 674 3074 Fragments arranged by size: 3074 939 828 674 552 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2752 4385 4402 4555 4740 5185 325 Size: 1255 1172 1633 17 153 185 445 1498 Fragments arranged by size: 1633 1498 1255 1172 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2074 4604 6316 Size: 274 80 33 51 1443 97 2530 1712 Cuts at: 6316 96 Size: 138 Fragments arranged by size: 2530 1712 1443 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3393 3425 6318 6350 3393 Size: 32 2893 32 3401 Fragments arranged by size: 3401 2893 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 2394 3447 4487 2394 Size: 1053 1040 4265 Fragments arranged by size: 4265 1053 1040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4166 276 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI