################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SfcI SfiI SfuI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AlwI 108 GATCC GGATC(4/5) Aor51HI 482 AGCGCT AGC^GCT AspHI 403 GAGCTC G[AT]GC[AT]^C AvaI 741 CTCGGG C^[CT]CG[GA]G AviII 353 TGCGCA TGC^GCA BbsI 601 GTCTTC GAAGAC(2/6) BbvCI 73 CCTCAGC CCTCAGC(-5/-2) BcgI 356 GCAAGGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 163 CCCGG CC^[GC]GG BglII 455 AGATCT A^GATCT BlpI 528 GCTGAGC GC^T[ACGT]AGC BpmI 622 CTGGAG CTGGAG(16/14) Bpu1102I 528 GCTGAGC GC^T[ACGT]AGC BpuAI 601 GTCTTC GAAGAC(2/6) BsaI 384 GGTCTC GGTCTC(1/5) BsaWI 289 TCCGGT [AT]^CCGG[AT] BsiHKAI 403 GAGCTC G[AT]GC[AT]^C BsmBI 442 GAGACG CGTCTC(1/5) BsmI 420 GAATGC GAATGC(1/-1) BsoBI 741 CTCGGG C^[CT]CG[GA]G BspMI 461 ACCTGC ACCTGC(4/8) CelII 528 GCTGAGC GC^T[ACGT]AGC DraII 300 AGGACCC [GA]G^G[ACGT]CC[CT] EaeI 566 TGGCCG [CT]^GGCC[GA] EarI 176 GAAGAG CTCTTC(1/4) Eco47III 482 AGCGCT AGC^GCT EcoNI 621 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 300 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 539 CCAAGG C^C[AT][AT]GG FspI 353 TGCGCA TGC^GCA Ksp632I 176 GAAGAG CTCTTC(1/4) KspI 516 CCGCGG CCGC^GG MaeII 758 ACGT A^CGT NciI 163 CCCGG CC^[GC]GG NspI 533 GCATGC [GA]CATG^[CT] PleI 179 GAGTC GAGTC(4/5) PpuMI 300 AGGACCC [GA]G^G[AT]CC[CT] SacI 403 GAGCTC GAGCT^C SacII 516 CCGCGG CCGC^GG SexAI 380 ACCAGGT A^CC[AT]GGT SgrAI 429 CGCCGGTG C[GA]^CCGG[CT]G SmlI 406 CTCAAG C^T[CT][GA]AG SphI 533 GCATGC GCATG^C StuI 327 AGGCCT AGG^CCT StyI 539 CCAAGG C^C[AT][AT]GG TfiI 286 GATTC G^A[AT]TC Tsp509I 252 AATT ^AATT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 645 GTAC GT^AC 780 GTAC BanI 157 GGCGCC G^G[CT][GA]CC 634 GGCGCC BanII 403 GAGCTC G[GA]GC[CT]^C 694 GAGCCC BbeI 157 GGCGCC GGCGC^C 634 GGCGCC BglI 515 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 586 GCCGGCCTGGC BseRI 475 GAGGAG GAGGAG(10/8) 715 GAGGAG BsgI 103 GTGCAG GTGCAG(16/14) 342 GTGCAG BstF5I 70 CATCC GGATG(2/0) 132 GGATG BstYI 107 AGATCC [GA]^GATC[CT] 455 AGATCT DpnII 108 GATC ^GATC 456 GATC DrdI 302 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 376 GACAACCAGGTC DsaI 188 CCACGG C^C[GA][CT]GG 516 CCGCGG FauI 136 GCGGG CCCGC(4/6) 518 GCGGG FokI 70 CATCC GGATG(9/13) 132 GGATG HgaI 91 GACGC GACGC(5/10) 493 GACGC HinfI 179 GAGTC G^A[ACGT]TC 286 GATTC KasI 157 GGCGCC G^GCGCC 634 GGCGCC MaeIII 51 GTCAC ^GT[ACGT]AC 293 GTGAC MboI 108 GATC ^GATC 456 GATC MboII 176 GAAGA GAAGA(8/7) 602 TCTTC MflI 107 AGATCC [GA]^GATC[CT] 455 AGATCT MslI 335 CATGTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 771 CACCACCATG NaeI 78 GCCGGC GCC^GGC 586 GCCGGC NarI 157 GGCGCC GG^CGCC 634 GGCGCC NdeII 108 GATC ^GATC 456 GATC NgoMIV 78 GCCGGC G^CCGGC 586 GCCGGC RsaI 645 GTAC GT^AC 780 GTAC Sau3AI 108 GATC ^GATC 456 GATC SfaNI 133 GATGC GCATC(5/9) 611 GCATC SfoI 157 GGCGCC GGC^GCC 634 GGCGCC Tsp45I 51 GTCAC ^GT[GC]AC 293 GTGAC TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^ 231 CAGTG XcmI 362 CCATCGGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 554 CCAATGGCCTGATGG XhoII 107 AGATCC [GA]^GATC[CT] 455 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BbvI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EcoRII Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV PvuII Sau96I ScrFI TseI