################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BanII BbeI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI TaqI TseI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 763 GTCTAC GT^[AC][GT]AC AocI 870 CCTGAGG CC^T[ACGT]AGG BalI 368 TGGCCA TGG^CCA BamHI 150 GGATCC G^GATCC BbrPI 145 CACGTG CAC^GTG BfaI 509 CTAG C^TAG BglI 260 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 145 CACGTG [CT]AC^GT[GA] BsaBI 210 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 900 GAGGAG GAGGAG(10/8) BsiEI 357 CGACCG CG[GA][CT]^CG BsmAI 158 GTCTC GTCTC(1/5) BsmBI 157 CGTCTC CGTCTC(1/5) BspMI 503 GCAGGT ACCTGC(4/8) BsrBI 792 CCGCTC CCGCTC(-3/-3) Bsu36I 870 CCTGAGG CC^T[ACGT]AGG Cac8I 188 GCCAGC GC[ACGT]^[ACGT]GC DrdI 444 GACACTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 870 CCTGAGG CC^T[ACGT]AGG EcoNI 497 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 257 CCGG C^CGG HindIII 297 AAGCTT A^AGCTT HpaII 257 CCGG C^CGG MaeI 509 CTAG C^TAG MaeII 146 ACGT A^CGT MaeIII 119 GTAAC ^GT[ACGT]AC MamI 210 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MseI 730 TTAA T^TAA MspA1I 800 CAGCTG C[AC]G^C[GT]G MspI 257 CCGG C^CGG MwoI 260 GCCAGAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 676 CATATG CA^TATG PleI 656 GAGTC GAGTC(4/5) PmaCI 145 CACGTG CAC^GTG PmlI 145 CACGTG CAC^GTG PstI 501 CTGCAG CTGCA^G PvuII 800 CAGCTG CAG^CTG SspI 69 AATATT AAT^ATT StuI 704 AGGCCT AGG^CCT Tru9I 730 TTAA T^TAA TspRI 446 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 455 CCATCAACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 399 CCGC CCGC(-3/-1) 792 CCGC AvaII 204 GGACC G^G[AT]CC 452 GGTCC BbsI 805 GAAGAC GAAGAC(2/6) 823 GTCTTC BpuAI 805 GAAGAC GAAGAC(2/6) 823 GTCTTC BsmFI 229 GTCCC GGGAC(10/14) 538 GTCCC Bsp1407I 212 TGTACA T^GTACA 562 TGTACA BsrGI 212 TGTACA T^GTACA 562 TGTACA BsrI 64 ACTGG ACTGG(1/-1) 447 ACTGG BstYI 150 GGATCC [GA]^GATC[CT] 812 AGATCC DdeI 184 CTCAG C^T[ACGT]AG 871 CTGAG DsaI 314 CCATGG C^C[GA][CT]GG 347 CCATGG MflI 150 GGATCC [GA]^GATC[CT] 812 AGATCC MslI 141 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 165 CACACCGATG NcoI 314 CCATGG C^CATGG 347 CCATGG NlaIV 150 GGATCC GG[ACGT]^[ACGT]CC 451 GGGTCC SfaNI 269 GCATC GCATC(5/9) 635 GCATC SspBI 212 TGTACA T^GTACA 562 TGTACA XhoII 150 GGATCC [GA]^GATC[CT] 812 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI AlwI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DpnII EaeI EcoRII EcoT14I FokI HaeIII HinfI HphI MboI MboII MnlI MvaI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfcI StyI TfiI