(Circular) (Six-base) MAPSORT of: pFN21AE5421.seq Check: 7872 from: 1 to: 5641 pFN21AE5421 5641 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2769 2984 1937 Size: 832 215 4594 Fragments arranged by size: 4594 832 215 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2829 3704 5088 5356 1632 Size: 720 477 875 1384 268 1917 Fragments arranged by size: 1917 1384 875 720 477 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 3384 4956 3384 Size: 1572 4069 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2966 1870 Size: 73 1023 4545 Fragments arranged by size: 4545 1023 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2565 2598 2565 Size: 33 5608 BamHI G'GATC_C Cuts at: 2155 2971 3338 2155 Size: 816 367 4458 Fragments arranged by size: 4458 816 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2962 4417 618 Size: 325 217 627 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 627 325 217 BanII G_rGCy'C Cuts at: 1391 2960 1391 Size: 1569 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2817 2821 928 Size: 971 918 4 3748 Fragments arranged by size: 3748 971 918 4 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 4252 136 Size: 107 122 71 631 605 599 1981 1525 Fragments arranged by size: 1981 1525 631 605 599 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2462 3372 4330 Size: 416 53 83 399 24 1045 910 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1045 958 910 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4301 1239 Size: 3062 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2152 3432 493 Size: 711 948 1280 2702 Fragments arranged by size: 2702 1280 948 711 BsaBI GATnn'nnATC Cuts at: 2219 5635 2219 Size: 3416 2225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4074 4472 4746 4893 5304 4074 Size: 398 274 147 411 4411 Fragments arranged by size: 4411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2920 1186 Size: 1734 3907 BseYI C'CCAG_C Cuts at: 2545 4844 5340 2545 Size: 2299 496 2846 Fragments arranged by size: 2846 2299 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2365 3091 3852 4880 1540 Size: 275 186 364 726 761 1028 2301 Fragments arranged by size: 2301 1028 761 726 364 275 186 BsiHKAI G_wGCw'C Cuts at: 3702 3787 4858 3702 Size: 85 1071 4485 Fragments arranged by size: 4485 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 BsmBI CGTCTCn'nnnn_ Cuts at: 2168 2168 Size: 5641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2960 3702 3787 4858 1391 Size: 399 1170 742 85 1071 2174 Fragments arranged by size: 2174 1170 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2996 844 Size: 1655 497 3489 Fragments arranged by size: 3489 1655 497 BsrBI CCG'CTC Cuts at: 1394 1823 2799 3528 1394 Size: 429 976 729 3507 Fragments arranged by size: 3507 976 729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 2217 2567 96 Size: 2121 350 3170 Fragments arranged by size: 3170 2121 350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 3701 4713 3701 Size: 1012 4629 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2155 2817 2971 3338 3723 3740 5192 5278 Size: 183 662 154 367 385 17 1452 86 Cuts at: 5278 5290 5636 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 662 385 367 346 183 154 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 5641 BtgI C'CryG_G Cuts at: 513 1065 2004 2319 2352 513 Size: 552 939 315 33 3802 Fragments arranged by size: 3802 939 552 315 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2553 487 Size: 683 56 1327 3575 Fragments arranged by size: 3575 1327 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2455 4648 809 Size: 507 1139 2193 1802 Fragments arranged by size: 2193 1802 1139 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2263 2373 Size: 54 1425 50 275 18 300 133 110 Cuts at: 2373 2656 3088 3977 5606 8 Size: 283 432 889 1629 43 Fragments arranged by size: 1629 1425 889 432 300 283 275 133 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2829 3704 4301 5088 5356 1239 Size: 393 720 477 875 597 787 268 1524 Fragments arranged by size: 1524 875 787 720 597 477 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2506 2506 Size: 5641 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 4788 1678 Size: 3110 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 2302 748 Size: 1554 4087 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2375 10 Size: 54 1425 343 543 3276 Fragments arranged by size: 3276 1425 543 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2174 3599 3958 4117 518 Size: 648 984 24 1425 359 159 2042 Fragments arranged by size: 2042 1425 984 648 359 159 24 MspA1I CmG'CkG Cuts at: 1606 2807 3057 3734 4882 5127 1606 Size: 1201 250 677 1148 245 2120 Fragments arranged by size: 2120 1201 1148 677 250 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2319 2352 513 Size: 552 939 315 33 3802 Fragments arranged by size: 3802 939 552 315 33 NdeI CA'TA_TG Cuts at: 387 2682 387 Size: 2295 3346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PmlI CAC'GTG Cuts at: 2152 2152 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PstI C_TGCA'G Cuts at: 830 2510 2993 830 Size: 1680 483 3478 Fragments arranged by size: 3478 1680 483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2807 2807 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2506 2739 2869 2989 4124 4805 4996 Size: 219 1461 233 130 120 1135 681 191 Cuts at: 4996 826 Size: 1471 Fragments arranged by size: 1471 1461 1135 681 233 219 191 130 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 2076 3565 5 Size: 47 2024 1489 2081 Fragments arranged by size: 2081 2024 1489 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4447 StyI C'CwwG_G Cuts at: 513 1065 2004 2319 2352 2469 3080 513 Size: 552 939 315 33 117 611 3074 Fragments arranged by size: 3074 939 611 552 315 117 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 2217 2567 Size: 274 80 33 51 1443 201 39 350 Cuts at: 2567 2606 3887 5599 96 Size: 39 1281 1712 138 Fragments arranged by size: 1712 1443 1281 350 274 201 138 80 51 39 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 5601 5633 2280 Size: 32 3289 32 2288 Fragments arranged by size: 3289 2288 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 2467 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI