################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstXI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MflI MluI MroI MseI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AocI 723 CCTCAGG CC^T[ACGT]AGG Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 737 GAGCTC G[AT]GC[AT]^C BalI 922 TGGCCA TGG^CCA BbrPI 166 CACGTG CAC^GTG BsaAI 166 CACGTG [CT]AC^GT[GA] BseRI 70 GAGGAG GAGGAG(10/8) BsiHKAI 737 GAGCTC G[AT]GC[AT]^C BsmAI 958 GAGAC GTCTC(1/5) Bsp1407I 803 TGTACA T^GTACA BsrGI 803 TGTACA T^GTACA BstEII 432 GGTGACC G^GT[ACGT]ACC BstPI 432 GGTGACC G^GT[ACGT]ACC Bsu36I 723 CCTCAGG CC^T[ACGT]AGG DpnII 978 GATC ^GATC EarI 358 CTCTTC CTCTTC(1/4) Eco81I 723 CCTCAGG CC^T[ACGT]AGG EcoNI 63 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 432 GGTGACC G^GT[ACGT]ACC HinfI 490 GACTC G^A[ACGT]TC HphI 432 GGTGA GGTGA(8/7) Ksp632I 358 CTCTTC CTCTTC(1/4) KspI 219 CCGCGG CCGC^GG MboI 978 GATC ^GATC MboII 359 TCTTC GAAGA(8/7) MfeI 918 CAATTG C^AATTG MluNI 922 TGGCCA TGG^CCA MscI 922 TGGCCA TGG^CCA MslI 1035 CATGAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 918 CAATTG C^AATTG NdeII 978 GATC ^GATC PleI 490 GACTC GAGTC(4/5) PmaCI 166 CACGTG CAC^GTG PmlI 166 CACGTG CAC^GTG SacI 737 GAGCTC GAGCT^C SacII 219 CCGCGG CCGC^GG SapI 357 GCTCTTC GCTCTTC(1/4) Sau3AI 978 GATC ^GATC SbfI 813 CCTGCAGG CCTGCA^GG Sse8387I 813 CCTGCAGG CCTGCA^GG SspBI 803 TGTACA T^GTACA StuI 527 AGGCCT AGG^CCT Tsp509I 919 AATT ^AATT TspRI 943 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 124 GTAC GT^AC 804 GTAC ApaI 173 GGGCCC GGGCC^C 624 GGGCCC BbeI 640 GGCGCC GGCGC^C 675 GGCGCC BlpI 108 GCTCAGC GC^T[ACGT]AGC 567 GCTGAGC Bpu1102I 108 GCTCAGC GC^T[ACGT]AGC 567 GCTGAGC BsrBI 213 CCGCTC CCGCTC(-3/-3) 516 GAGCGG BssSI 511 CACGAG CACGAG(-5/-1) 1075 CTCGTG CelII 108 GCTCAGC GC^T[ACGT]AGC 567 GCTGAGC DsaI 204 CCACGG C^C[GA][CT]GG 219 CCGCGG EaeI 254 TGGCCG [CT]^GGCC[GA] 922 TGGCCA EcoT14I 377 CCAAGG C^C[AT][AT]GG 1081 CCAAGG KasI 640 GGCGCC G^GCGCC 675 GGCGCC MaeII 167 ACGT A^CGT 536 ACGT NaeI 256 GCCGGC GCC^GGC 572 GCCGGC NarI 640 GGCGCC GG^CGCC 675 GGCGCC NgoMIV 256 GCCGGC G^CCGGC 572 GCCGGC PflMI 645 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 833 CCAAGAACTGG PstI 103 CTGCAG CTGCA^G 814 CTGCAG RsaI 124 GTAC GT^AC 804 GTAC SfaNI 618 GCATC GCATC(5/9) 986 GCATC SfoI 640 GGCGCC GGC^GCC 675 GGCGCC StyI 377 CCAAGG C^C[AT][AT]GG 1081 CCAAGG Van91I 645 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 833 CCAAGAACTGG XcmI 326 CCAACAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1007 CCAGGCCCCAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeIII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PvuII Sau96I ScrFI SfcI SmaI TaqI TseI Tsp45I TthHB8I XmaCI XmaI