(Circular) (Six-base) MAPSORT of: pFN21AE0400.seq Check: 8285 from: 1 to: 5803 pFN21AE0400 5803 bp With 182 enzymes: SgfI * March 28, 2012 12:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2304 2900 2304 Size: 596 5207 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2784 3866 5250 5518 1632 Size: 1152 1082 1384 268 1917 Fragments arranged by size: 1917 1384 1152 1082 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2114 2312 1184 Size: 930 198 4675 Fragments arranged by size: 4675 930 198 AlwNI CAG_nnn'CTG Cuts at: 2086 2323 2572 2593 3546 5118 2086 Size: 237 249 21 953 1572 2771 Fragments arranged by size: 2771 1572 953 249 237 21 ApaI G_GGCC'C Cuts at: 2182 2633 2182 Size: 451 5352 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 3112 3338 3490 5599 1493 Size: 1619 226 152 2109 1697 Fragments arranged by size: 2109 1697 1619 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2433 2860 3128 1870 Size: 73 114 376 427 268 4545 Fragments arranged by size: 4545 427 376 268 114 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2645 2680 2783 2888 Size: 325 217 627 335 523 35 103 105 Cuts at: 2888 3124 4579 618 Size: 236 1455 1842 Fragments arranged by size: 1842 1455 627 523 335 325 236 217 105 103 35 BanII G_rGCy'C Cuts at: 1391 2182 2633 2703 2746 2859 3122 1391 Size: 791 451 70 43 113 263 4072 Fragments arranged by size: 4072 791 451 263 113 70 43 BbeI G_GCGC'C Cuts at: 2649 2684 2649 Size: 35 5768 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4832 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2017 2689 3091 Size: 107 122 71 631 605 345 672 402 Cuts at: 3091 4414 136 Size: 1323 1525 Fragments arranged by size: 1525 1323 672 631 605 402 345 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 2114 2573 3220 2114 Size: 459 647 4697 Fragments arranged by size: 4697 647 459 Bme1580I G_kGCm'C Cuts at: 1790 2032 2127 2182 2633 2786 2891 3864 5020 Size: 242 95 55 451 153 105 973 1156 Cuts at: 5020 1790 Size: 2573 Fragments arranged by size: 2573 1156 973 451 242 153 105 95 55 BmgBI CAC'GTC Cuts at: 1318 2542 1318 Size: 1224 4579 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 2854 3534 Size: 416 53 83 399 24 759 678 680 Cuts at: 3534 4492 442 Size: 958 1753 Fragments arranged by size: 1753 958 759 680 678 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2986 2986 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2482 2729 2873 3080 4463 1239 Size: 1243 247 144 207 1383 2579 Fragments arranged by size: 2579 1383 1243 247 207 144 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2173 3594 493 Size: 711 969 1421 2702 Fragments arranged by size: 2702 1421 969 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2276 2639 2646 2681 Size: 53 83 186 936 743 363 7 35 Cuts at: 2681 3610 3992 4627 275 Size: 929 382 635 1451 Fragments arranged by size: 1451 936 929 743 635 382 363 186 83 53 35 7 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2456 2468 2486 2498 1178 Size: 30 165 30 1053 12 18 12 4483 Fragments arranged by size: 4483 1053 165 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 1186 Size: 904 4899 BseYI C'CCAG_C Cuts at: 2025 2703 5006 5502 2025 Size: 678 2303 496 2326 Fragments arranged by size: 2326 2303 678 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2378 2633 2699 3068 3076 1163 Size: 573 630 12 255 66 369 8 3890 Fragments arranged by size: 3890 630 573 369 255 66 12 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2746 3864 3949 5020 2746 Size: 1118 85 1071 3529 Fragments arranged by size: 3529 1118 1071 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2127 2182 2633 2703 2746 2786 Size: 399 242 95 55 451 70 43 40 Cuts at: 2786 2859 2891 3122 3864 3949 5020 1391 Size: 73 32 231 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 399 242 231 95 85 73 70 55 43 40 32 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2256 2304 2826 2900 3158 844 Size: 1412 48 522 74 258 3489 Fragments arranged by size: 3489 1412 522 258 74 48 BsrBI CCG'CTC Cuts at: 1394 1823 2220 2523 3690 1394 Size: 429 397 303 1167 3507 Fragments arranged by size: 3507 1167 429 397 303 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2261 2374 2577 4447 4634 1700 Size: 110 451 113 203 1870 187 2869 Fragments arranged by size: 2869 1870 451 203 187 113 110 BsrGI T'GTAC_A Cuts at: 96 2808 96 Size: 2712 3091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 2516 3080 3863 4875 2516 Size: 564 783 1012 3444 Fragments arranged by size: 3444 1012 783 564 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 2353 2593 2790 794 Size: 1529 30 240 197 3807 Fragments arranged by size: 3807 1529 240 197 30 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 2437 1184 Size: 1253 4550 BstYI r'GATC_y Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798 Size: 1161 367 385 17 1452 86 12 346 Cuts at: 5798 1972 Size: 1977 Fragments arranged by size: 1977 1452 1161 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2729 2729 Size: 5803 BtgI C'CryG_G Cuts at: 513 1065 2209 2224 513 Size: 552 1144 15 4092 Fragments arranged by size: 4092 1144 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 3319 4113 4133 3319 Size: 794 20 4989 Fragments arranged by size: 4989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2927 3250 Size: 54 1425 50 275 18 429 668 323 Cuts at: 3250 4139 5768 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 668 429 323 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 3733 1353 Size: 1016 1364 3423 Fragments arranged by size: 3423 1364 1016 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4390 4764 4910 387 Size: 1064 138 2801 374 146 1280 Fragments arranged by size: 2801 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2744 2744 Size: 5803 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2482 2729 2784 2873 3080 3866 4463 Size: 393 850 247 55 89 207 786 597 Cuts at: 4463 5250 5518 1239 Size: 787 268 1524 Fragments arranged by size: 1524 850 787 786 597 393 268 247 207 89 55 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2072 Size: 5803 EcoO109I rG'GnC_Cy Cuts at: 2178 2911 3015 2178 Size: 733 104 4966 Fragments arranged by size: 4966 733 104 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 2427 2649 2684 3080 4950 1678 Size: 749 222 35 396 1870 2531 Fragments arranged by size: 2531 1870 749 396 222 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 2608 2640 2937 Size: 32 135 32 884 32 120 32 297 Cuts at: 2937 2969 4444 4476 4518 4550 1373 Size: 32 1475 32 42 32 2626 Fragments arranged by size: 2626 1475 884 297 135 120 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KasI G'GCGC_C Cuts at: 2645 2680 2645 Size: 35 5768 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 2923 3402 2923 Size: 479 5324 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 2499 4917 5101 1305 Size: 954 240 2418 184 2007 Fragments arranged by size: 2418 2007 954 240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2929 10 Size: 54 1425 343 1097 2884 Fragments arranged by size: 2884 1425 1097 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3044 3761 4120 4279 518 Size: 648 1878 717 359 159 2042 Fragments arranged by size: 2042 1878 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2226 2320 2572 2593 2635 2707 2790 Size: 501 119 94 252 21 42 72 83 Cuts at: 2790 3060 3219 3896 5044 5289 1606 Size: 270 159 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 270 252 245 159 119 94 83 72 42 21 NaeI GCC'GGC Cuts at: 2263 2579 2263 Size: 316 5487 NarI GG'CG_CC Cuts at: 2646 2681 2646 Size: 35 5768 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NgoMIV G'CCGG_C Cuts at: 2261 2577 2261 Size: 316 5487 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 3161 4706 1132 Size: 97 1932 1545 2229 Fragments arranged by size: 2229 1932 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 2844 1108 Size: 1548 188 4067 Fragments arranged by size: 4067 1548 188 PfoI T'CCnGG_A Cuts at: 2367 2367 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PmlI CAC'GTG Cuts at: 2173 2173 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2178 2629 2178 Size: 451 5352 PstI C_TGCA'G Cuts at: 830 2112 2823 3155 830 Size: 1282 711 332 3478 Fragments arranged by size: 3478 1282 711 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2107 2572 2707 3060 2107 Size: 465 135 353 4850 Fragments arranged by size: 4850 465 353 135 SacI G_AGCT'C Cuts at: 2746 2746 Size: 5803 SacII CC_GC'GG Cuts at: 2227 2227 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SapI GCTCTTCn'nnn_ Cuts at: 2369 2369 Size: 5803 SbfI CC_TGCA'GG Cuts at: 2823 3155 2823 Size: 332 5471 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2108 2600 2819 3151 4286 4967 5158 Size: 219 1063 492 219 332 1135 681 191 Cuts at: 5158 826 Size: 1471 Fragments arranged by size: 1471 1135 1063 681 492 332 219 219 191 SfoI GGC'GCC Cuts at: 2647 2682 2647 Size: 35 5768 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 2059 2435 2862 3130 1872 Size: 187 376 427 268 4545 Fragments arranged by size: 4545 427 376 268 187 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 2534 1517 Size: 1017 4786 StyI C'CwwG_G Cuts at: 513 1065 2382 3086 3242 513 Size: 552 1317 704 156 3074 Fragments arranged by size: 3074 1317 704 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2026 2087 2561 2792 2836 3830 3847 Size: 1255 446 61 474 231 44 994 17 Cuts at: 3847 4000 4185 4630 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 994 474 446 445 231 185 153 61 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2808 4049 5761 Size: 274 80 33 51 1443 831 1241 1712 Cuts at: 5761 96 Size: 138 Fragments arranged by size: 1712 1443 1241 831 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5763 5795 5763 Size: 32 5771 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 3019 2338 Size: 681 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 2057 2433 2860 3128 1870 Size: 187 376 427 268 4545 Fragments arranged by size: 4545 427 376 268 187 XmnI GAAnn'nnTTC Cuts at: 2534 3932 2534 Size: 1398 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI