################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BbeI BbrPI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII CfoI ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AciI 411 CCGC CCGC(-3/-1) AgeI 597 ACCGGT A^CCGGT AlwNI 37 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 541 CCTCAGG CC^T[ACGT]AGG Asp700I 797 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 279 GGATCC G^GATCC BbsI 1287 GAAGAC GAAGAC(2/6) BbvCI 385 CCTCAGC CCTCAGC(-5/-2) BciVI 1118 GTATCC GTATCC(6/5) BclI 995 TGATCA T^GATCA BpuAI 1287 GAAGAC GAAGAC(2/6) BsaWI 597 ACCGGT [AT]^CCGG[AT] BsmBI 127 CGTCTC CGTCTC(1/5) Bsp1407I 242 TGTACA T^GTACA BsrDI 413 GCAATG GCAATG(2/0) BsrFI 597 ACCGGT [GA]^CCGG[CT] BsrGI 242 TGTACA T^GTACA BssSI 999 CACGAG CACGAG(-5/-1) BstF5I 1245 GGATG GGATG(2/0) BstXI 350 CCAAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 541 CCTCAGG CC^T[ACGT]AGG Cac8I 108 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 597 ACCGGT [GA]^CCGG[CT] DraIII 15 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 115 GAAGAG CTCTTC(1/4) Eco81I 541 CCTCAGG CC^T[ACGT]AGG FbaI 995 TGATCA T^GATCA FokI 1245 GGATG GGATG(9/13) HapII 598 CCGG C^CGG HpaII 598 CCGG C^CGG Ksp632I 115 GAAGAG CTCTTC(1/4) MaeII 731 ACGT A^CGT MfeI 626 CAATTG C^AATTG MseI 98 TTAA T^TAA MslI 987 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 598 CCGG C^CGG MunI 626 CAATTG C^AATTG MwoI 93 GCAGTTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 666 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 597 ACCGGT A^CCGGT PleI 1095 GACTC GAGTC(4/5) PstI 43 CTGCAG CTGCA^G ScaI 1132 AGTACT AGT^ACT SspBI 242 TGTACA T^GTACA TaqI 833 TCGA T^CGA Tru9I 98 TTAA T^TAA TthHB8I 833 TCGA T^CGA Van91I 666 CCAAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 797 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 568 AAATTT [GA]^AATT[CT] 882 GAATTT ApoI 568 AAATTT [GA]^AATT[CT] 882 GAATTT AvaII 77 GGACC G^G[AT]CC 1249 GGACC BanI 394 GGTGCC G^G[CT][GA]CC 981 GGCACC BbvI 341 GCAGC GCAGC(8/12) 613 GCTGC BfaI 407 CTAG C^TAG 922 CTAG BsaJI 704 CCCTGG C^C[ACGT][ACGT]GG 1260 CCCTGG BseRI 468 CTCCTC GAGGAG(10/8) 539 CTCCTC BstYI 279 GGATCC [GA]^GATC[CT] 952 AGATCC MaeI 407 CTAG C^TAG 922 CTAG MflI 279 GGATCC [GA]^GATC[CT] 952 AGATCC TseI 341 GCAGC G^C[AT]GC 613 GCTGC XhoII 279 GGATCC [GA]^GATC[CT] 952 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AfaI AluI AlwI BanII BmyI BpmI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HindIII HinfI HphI ItaI MaeIII MboI MboII MnlI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tsp45I Tsp509I TspRI