(Circular) (Six-base) MAPSORT of: pFN21AE4358.seq Check: 3180 from: 1 to: 5830 pFN21AE4358 5830 bp With 182 enzymes: SgfI * March 28, 2012 13:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 3062 3893 5277 5545 1632 Size: 537 893 831 1384 268 1917 Fragments arranged by size: 1917 1384 893 831 537 268 AfeI AGC'GCT Cuts at: 2975 2975 Size: 5830 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 2772 4661 2772 Size: 1889 3941 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2891 1166 Size: 1725 4105 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2566 1184 Size: 1382 4448 AlwNI CAG_nnn'CTG Cuts at: 2408 3573 5145 2408 Size: 1165 1572 3093 Fragments arranged by size: 3093 1572 1165 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2964 2990 3083 3139 3365 3517 5626 1493 Size: 1471 26 93 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 1471 226 152 93 56 26 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 2050 3149 1391 Size: 659 1099 4072 Fragments arranged by size: 4072 1099 659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 4757 Fragments arranged by size: 4757 971 102 BbvCI CC'TCA_GC Cuts at: 2555 2868 2555 Size: 313 5517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2904 3102 4441 Size: 107 122 71 631 605 1232 198 1339 Cuts at: 4441 136 Size: 1525 Fragments arranged by size: 1525 1339 1232 631 605 198 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 2175 2847 3891 5047 1790 Size: 385 672 1044 1156 2573 Fragments arranged by size: 2573 1156 1044 672 385 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2102 2162 3561 Size: 416 53 83 399 24 685 60 1399 Cuts at: 3561 4519 442 Size: 958 1753 Fragments arranged by size: 1753 1399 958 685 416 399 83 60 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 2620 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2961 4490 1239 Size: 1722 1529 2579 Fragments arranged by size: 2579 1722 1529 Bpu10I CC'TnA_GC Cuts at: 2555 2787 2868 2970 2555 Size: 232 81 102 5415 Fragments arranged by size: 5415 232 102 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2851 3955 4820 5118 2851 Size: 1104 865 298 3563 Fragments arranged by size: 3563 1104 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 2933 5824 2933 Size: 2891 2939 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2838 3637 4019 4654 Size: 53 83 186 936 1305 799 382 635 Cuts at: 4654 275 Size: 1451 Fragments arranged by size: 1451 1305 936 799 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2772 4263 4661 4935 5082 5493 2772 Size: 1491 398 274 147 411 3109 Fragments arranged by size: 3109 1491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2204 1186 Size: 1015 3 4812 Fragments arranged by size: 4812 1015 3 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 1163 Size: 573 826 4431 Fragments arranged by size: 4431 826 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 2550 3891 3976 5047 2304 Size: 246 1341 85 1071 3087 Fragments arranged by size: 3087 1341 1071 246 85 BsmI GAATG_Cn' Cuts at: 2394 3341 3434 2394 Size: 947 93 4790 Fragments arranged by size: 4790 947 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2175 2304 2550 2847 3149 3891 Size: 399 260 125 129 246 297 302 742 Cuts at: 3891 3976 5047 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 297 260 246 129 125 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2772 2793 4474 4661 1700 Size: 110 962 21 1681 187 2869 Fragments arranged by size: 2869 1681 962 187 110 21 BsrGI T'GTAC_A Cuts at: 96 2256 2931 96 Size: 2160 675 2995 Fragments arranged by size: 2995 2160 675 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5830 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2432 2758 2893 3062 2432 Size: 326 135 169 5200 Fragments arranged by size: 5200 326 169 135 BstYI r'GATC_y Cuts at: 1972 2021 3160 3527 3912 3929 5381 5467 5479 Size: 49 1139 367 385 17 1452 86 12 Cuts at: 5479 5825 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1139 385 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2232 2705 513 Size: 552 1167 473 3638 Fragments arranged by size: 3638 1167 552 473 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2550 2771 3346 4140 4160 2550 Size: 221 575 794 20 4220 Fragments arranged by size: 4220 794 575 221 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795 Size: 54 1425 50 275 18 1447 889 1629 Cuts at: 5795 8 Size: 43 Fragments arranged by size: 1629 1447 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2148 2178 2241 2649 3760 1353 Size: 750 45 30 63 408 1111 3423 Fragments arranged by size: 3423 1111 750 408 63 45 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2961 3062 3893 4490 5277 5545 Size: 393 537 792 101 831 597 787 268 Cuts at: 5545 1239 Size: 1524 Fragments arranged by size: 1524 831 792 787 597 537 393 268 101 EcoO109I rG'GnC_Cy Cuts at: 2096 2096 Size: 5830 EcoRI G'AATT_C Cuts at: 2964 3083 3139 3517 2964 Size: 119 56 378 5277 Fragments arranged by size: 5277 378 119 56 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2404 2404 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2977 4977 1678 Size: 1299 2000 2531 Fragments arranged by size: 2531 2000 1299 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2258 3096 4944 5128 1305 Size: 953 838 1848 184 2007 Fragments arranged by size: 2007 1848 953 838 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 2891 2924 3060 3788 4147 4306 Size: 648 1590 135 33 136 728 359 159 Cuts at: 4306 518 Size: 2042 Fragments arranged by size: 2042 1590 728 648 359 159 136 135 33 MspA1I CmG'CkG Cuts at: 1606 3246 3923 5071 5316 1606 Size: 1640 677 1148 245 2120 Fragments arranged by size: 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2232 513 Size: 552 1167 4111 Fragments arranged by size: 4111 1167 552 NdeI CA'TA_TG Cuts at: 387 2293 2999 387 Size: 1906 706 3218 Fragments arranged by size: 3218 1906 706 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2166 3188 4733 1132 Size: 97 937 1022 1545 2229 Fragments arranged by size: 2229 1545 1022 937 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2233 3031 1108 Size: 1125 798 3907 Fragments arranged by size: 3907 1125 798 PfoI T'CCnGG_A Cuts at: 2983 2983 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2096 2096 Size: 5830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SanDI GG'GwC_CC Cuts at: 2096 2096 Size: 5830 SapI GCTCTTCn'nnn_ Cuts at: 2148 2649 2148 Size: 501 5329 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2504 3178 4313 4994 5185 826 Size: 219 1459 674 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 674 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2830 3934 4835 5097 820 Size: 197 926 887 1104 901 262 1553 Fragments arranged by size: 1553 1104 926 901 887 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2232 3269 513 Size: 552 1167 1037 3074 Fragments arranged by size: 3074 1167 1037 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 2528 3857 3874 4027 4212 4657 Size: 1255 575 373 1329 17 153 185 445 Cuts at: 4657 325 Size: 1498 Fragments arranged by size: 1498 1329 1255 575 445 373 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 2487 2931 Size: 274 80 33 51 1443 279 231 444 Cuts at: 2931 3110 4076 5788 96 Size: 179 966 1712 138 Fragments arranged by size: 1712 1443 966 444 279 274 231 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 5790 5822 2013 Size: 32 3745 32 2021 Fragments arranged by size: 3745 2021 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2239 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI