Restriction map for
pFN21AE9442
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AviII BcgI BciVI BclI
BfrI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI MfeI MseI MunI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SexAI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 758 TCCGGA T^CCGGA
AhdI 1872 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1872 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2070 CCTAGG C^CTAGG
BalI 752 TGGCCA TGG^CCA
BamHI 1406 GGATCC G^GATCC
BglII 966 AGATCT A^GATCT
BlnI 2070 CCTAGG C^CTAGG
BlpI 1188 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1188 GCTGAGC GC^T[ACGT]AGC
BsaBI 2623 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 758 TCCGGA T^CCGGA
BsiEI 2564 CGGCCG CG[GA][CT]^CG
BspEI 758 TCCGGA T^CCGGA
BsrDI 1733 GCAATG GCAATG(2/0)
BstAPI 420 GCAGTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1188 GCTGAGC GC^T[ACGT]AGC
EagI 2564 CGGCCG C^GGCCG
Eam1105I 1872 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2564 CGGCCG C^GGCCG
Eco52I 2564 CGGCCG C^GGCCG
EcoNI 1639 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1336 ATGCAT ATGCA^T
HindIII 2642 AAGCTT A^AGCTT
KspI 2275 CCGCGG CCGC^GG
MamI 2623 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 611 ACGCGT A^CGCGT
MluNI 752 TGGCCA TGG^CCA
MroI 758 TCCGGA T^CCGGA
MscI 752 TGGCCA TGG^CCA
NaeI 64 GCCGGC GCC^GGC
NgoMIV 64 GCCGGC G^CCGGC
NsiI 1336 ATGCAT ATGCA^T
SacII 2275 CCGCGG CCGC^GG
SbfI 561 CCTGCAGG CCTGCA^GG
ScaI 1947 AGTACT AGT^ACT
SfiI 1698 GGCCGTGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 87 CCCGGG CCC^GGG
Sse8387I 561 CCTGCAGG CCTGCA^GG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 70 GGCGCC G[GA]^CG[CT]C
1816 GGCGCC
AflIII 611 ACGCGT A^C[GA][CT]GT
2895 ACACGT
AocI 1243 CCTGAGG CC^T[ACGT]AGG
2167 CCTGAGG
Asp700I 1492 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAACTCCTTC
BbeI 70 GGCGCC GGCGC^C
1816 GGCGCC
BbrPI 2320 CACGTG CAC^GTG
2896 CACGTG
BbsI 1492 GAAGAC GAAGAC(2/6)
1518 GTCTTC
BpuAI 1492 GAAGAC GAAGAC(2/6)
1518 GTCTTC
BsaAI 2320 CACGTG [CT]AC^GT[GA]
2896 CACGTG
BsaHI 70 GGCGCC G[GA]^CG[CT]C
1816 GGCGCC
BsaI 2674 GAGACC GGTCTC(1/5)
334 GGTCTC
BsaWI 758 TCCGGA [AT]^CCGG[AT]
1229 TCCGGT
BsmI 325 GCATTC GAATGC(1/-1)
898 GCATTC
BsrFI 64 GCCGGC [GA]^CCGG[CT]
2242 GCCGGT
Bsu36I 1243 CCTGAGG CC^T[ACGT]AGG
2167 CCTGAGG
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
2242 GCCGGT
Eco81I 1243 CCTGAGG CC^T[ACGT]AGG
2167 CCTGAGG
HgaI 285 GCGTC GACGC(5/10)
2003 GCGTC
Hin1I 70 GGCGCC G[GA]^CG[CT]C
1816 GGCGCC
KasI 70 GGCGCC G^GCGCC
1816 GGCGCC
MaeII 2321 ACGT A^CGT
2897 ACGT
NarI 70 GGCGCC GG^CGCC
1816 GGCGCC
PmaCI 2320 CACGTG CAC^GTG
2896 CACGTG
PmlI 2320 CACGTG CAC^GTG
2896 CACGTG
SapI 2580 GAAGAGC GCTCTTC(1/4)
2484 GCTCTTC
SfoI 70 GGCGCC GGC^GCC
1816 GGCGCC
XmnI 1492 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2217 GAACTCCTTC
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII