(Circular) (Six-base) MAPSORT of: pFN21AE9442.seq Check: 899 from: 1 to: 7606 pFN21AE9442 7606 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2277 4832 2277 Size: 2555 5051 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5416 278 Size: 53 83 186 936 3880 2468 Fragments arranged by size: 3880 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4949 1937 Size: 3012 4594 Acc65I G'GTAC_C Cuts at: 4927 4927 Size: 7606 AclI AA'CG_TT Cuts at: 1563 5733 6106 1563 Size: 4170 373 3063 Fragments arranged by size: 4170 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 3169 3368 3516 4674 5669 7053 7321 Size: 599 938 199 148 1158 995 1384 268 Cuts at: 7321 1632 Size: 1917 Fragments arranged by size: 1917 1384 1158 995 938 599 268 199 148 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7409 AflIII A'CryG_T Cuts at: 1203 2616 4900 6505 7482 1203 Size: 1413 2284 1605 977 1327 Fragments arranged by size: 2284 1605 1413 1327 977 AgeI A'CCGG_T Cuts at: 6437 6437 Size: 7606 AhdI GACnn_n'nnGTC Cuts at: 3882 6335 3882 Size: 2453 5153 AleI CACnn'nnGTG Cuts at: 1166 3627 1166 Size: 2461 5145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3044 4018 1184 Size: 1860 974 4772 Fragments arranged by size: 4772 1860 974 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2777 2778 2809 2810 2777 Size: 1 31 1 7573 Fragments arranged by size: 7573 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2431 2677 2788 2914 3583 4612 5349 6921 2431 Size: 246 111 126 669 1029 737 1572 3116 Fragments arranged by size: 3116 1572 1029 737 669 246 126 111 ApaI G_GGCC'C Cuts at: 2100 2548 4554 4732 2100 Size: 448 2006 178 4974 Fragments arranged by size: 4974 2006 448 178 ApaLI G'TGCA_C Cuts at: 5663 6819 5663 Size: 1156 6450 ApoI r'AATT_y Cuts at: 1493 4915 5141 5293 7402 1493 Size: 3422 226 152 2109 1697 Fragments arranged by size: 3422 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6160 160 Size: 872 5128 1606 Fragments arranged by size: 5128 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2168 4418 4931 1870 Size: 73 149 76 2250 513 4545 Fragments arranged by size: 4545 2250 513 149 76 73 AvrII C'CTAG_G Cuts at: 4075 4075 Size: 7606 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4466 4499 4466 Size: 33 7573 BamHI G'GATC_C Cuts at: 3411 4936 5303 3411 Size: 1525 367 5714 Fragments arranged by size: 5714 1525 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2945 2981 3421 3821 Size: 325 217 627 288 870 36 440 400 Cuts at: 3821 4250 4927 6382 618 Size: 429 677 1455 1842 Fragments arranged by size: 1842 1455 870 677 627 440 429 400 325 288 217 36 BanII G_rGCy'C Cuts at: 1391 2025 2100 2169 2548 3200 3594 3907 4356 Size: 634 75 69 379 652 394 313 449 Cuts at: 4356 4554 4592 4732 4925 1391 Size: 198 38 140 193 4072 Fragments arranged by size: 4072 652 634 449 394 379 313 198 193 140 75 69 38 BbeI G_GCGC'C Cuts at: 2079 3825 2079 Size: 1746 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3504 3516 928 Size: 971 1605 12 5018 Fragments arranged by size: 5018 1605 971 12 BbvCI CC'TCA_GC Cuts at: 2222 4379 4817 2222 Size: 2157 438 5011 Fragments arranged by size: 5011 2157 438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5797 5831 1903 Size: 34 50 34 3776 34 3678 Fragments arranged by size: 3776 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5498 6708 5498 Size: 1210 6396 BclI T'GATC_A Cuts at: 1548 1548 Size: 7606 BfrBI ATG'CAT Cuts at: 3343 3343 Size: 7606 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 2570 3710 Size: 107 122 71 631 605 439 459 1140 Cuts at: 3710 4672 6217 136 Size: 962 1545 1525 Fragments arranged by size: 1545 1525 1140 962 631 605 459 439 122 107 71 BglII A'GATC_T Cuts at: 2971 7601 2971 Size: 4630 2976 BlpI GC'TnA_GC Cuts at: 3194 5023 3194 Size: 1829 5777 Bme1580I G_kGCm'C Cuts at: 1790 2100 2548 4554 4732 5667 6823 1790 Size: 310 448 2006 178 935 1156 2573 Fragments arranged by size: 2573 2006 1156 935 448 310 178 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2798 3621 5337 Size: 416 53 83 399 24 1381 823 1716 Cuts at: 5337 6295 442 Size: 958 1753 Fragments arranged by size: 1753 1716 1381 958 823 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7606 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3274 4606 3274 Size: 1332 6274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2732 3014 3437 3578 3620 3633 4601 4697 Size: 1493 282 423 141 42 13 968 96 Cuts at: 4697 4760 6266 1239 Size: 63 1506 2579 Fragments arranged by size: 2579 1506 1493 968 423 282 141 96 63 42 13 Bpu10I CC'TnA_GC Cuts at: 2222 4379 4817 2222 Size: 2157 438 5011 Fragments arranged by size: 5011 2157 438 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5731 6596 6894 5731 Size: 865 298 6443 Fragments arranged by size: 6443 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2345 4673 6269 882 Size: 617 846 2328 1596 2219 Fragments arranged by size: 2328 2219 1596 846 617 BsaAI yAC'GTr Cuts at: 493 1204 4327 4903 5397 493 Size: 711 3123 576 494 2702 Fragments arranged by size: 3123 2702 711 576 494 BsaBI GATnn'nnATC Cuts at: 4632 7600 4632 Size: 2968 4638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 3822 5413 5795 Size: 53 83 186 936 543 1746 1591 382 Cuts at: 5795 6430 275 Size: 635 1451 Fragments arranged by size: 1746 1591 1451 936 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2763 3234 6039 6437 6711 6858 7269 2763 Size: 471 2805 398 274 147 411 3100 Fragments arranged by size: 3100 2805 471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 4127 4157 1178 Size: 30 165 30 1035 30 1659 30 4627 Fragments arranged by size: 4627 1659 1035 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2485 3113 3533 4346 1186 Size: 1111 188 628 420 813 4446 Fragments arranged by size: 4446 1111 813 628 420 188 BseYI C'CCAG_C Cuts at: 2009 2082 2380 3107 3687 3909 4396 4658 6809 Size: 73 298 727 580 222 487 262 2151 Cuts at: 6809 7305 2009 Size: 496 2310 Fragments arranged by size: 2310 2151 727 580 496 487 298 262 222 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2687 3098 3251 4019 4024 1163 Size: 573 951 411 153 768 5 4745 Fragments arranged by size: 4745 951 768 573 411 153 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4572 5056 5817 6845 1540 Size: 275 186 2571 484 761 1028 2301 Fragments arranged by size: 2571 2301 1028 761 484 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 3200 3594 3735 4592 5667 5752 6823 2169 Size: 1031 394 141 857 1075 85 1071 2952 Fragments arranged by size: 2952 1075 1071 1031 857 394 141 85 BsmI GAATG_Cn' Cuts at: 2330 2903 5117 5210 2330 Size: 573 2214 93 4726 Fragments arranged by size: 4726 2214 573 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 3908 4015 4021 2297 Size: 1611 107 6 5882 Fragments arranged by size: 5882 1611 107 6 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2100 2169 2548 3200 3594 3735 Size: 399 235 75 69 379 652 394 141 Cuts at: 3735 3907 4356 4554 4592 4732 4925 5667 5752 Size: 172 449 198 38 140 193 742 85 Cuts at: 5752 6823 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 652 449 399 394 379 235 198 193 172 141 140 85 75 69 38 BspEI T'CCGG_A Cuts at: 2763 7269 2763 Size: 4506 3100 BspHI T'CATG_A Cuts at: 5495 7225 5495 Size: 1730 5876 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 2922 4284 4494 4832 4961 844 Size: 1433 645 1362 210 338 129 3489 Fragments arranged by size: 3489 1433 1362 645 338 210 129 BsrBI CCG'CTC Cuts at: 1394 1823 5493 1394 Size: 429 3670 3507 Fragments arranged by size: 3670 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3745 6101 6275 65 Size: 1183 2497 2356 174 1396 Fragments arranged by size: 2497 2356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2069 4247 6250 6437 1700 Size: 110 259 2178 2003 187 2869 Fragments arranged by size: 2869 2178 2003 259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7606 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7606 BssSI C'ACGA_G Cuts at: 5666 6678 5666 Size: 1012 6594 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 794 Size: 1637 5969 BstBI TT'CG_AA Cuts at: 6410 6410 Size: 7606 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7606 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2641 2992 2581 Size: 60 351 7195 Fragments arranged by size: 7195 351 60 BstYI r'GATC_y Cuts at: 1972 2532 2971 2997 3214 3411 4633 4829 4936 Size: 560 439 26 217 197 1222 196 107 Cuts at: 4936 5303 5688 5705 7157 7243 7255 7601 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1222 560 439 385 367 346 217 197 196 107 86 26 17 12 Bsu36I CC'TnA_GG Cuts at: 3249 4173 3249 Size: 924 6682 BtgI C'CryG_G Cuts at: 513 1065 2949 4203 4280 4314 4368 4797 513 Size: 552 1884 1254 77 34 54 429 3322 Fragments arranged by size: 3322 1884 1254 552 429 77 54 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6867 Fragments arranged by size: 6867 683 56 BtsI GCAGTG_nn' Cuts at: 2122 3311 3491 4002 4007 4109 4475 5122 5916 Size: 1189 180 511 5 102 366 647 794 Cuts at: 5916 5936 2122 Size: 20 3792 Fragments arranged by size: 3792 1189 794 647 511 366 180 102 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7606 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7571 DraI TTT'AAA Cuts at: 4910 4910 Size: 7606 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6613 809 Size: 507 5297 1802 Fragments arranged by size: 5297 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2757 3702 3990 Size: 54 1425 50 275 18 927 945 288 Cuts at: 3990 4101 4245 4569 5053 5942 7571 8 Size: 111 144 324 484 889 1629 43 Fragments arranged by size: 1629 1425 945 927 889 484 324 288 275 144 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4569 5053 1537 Size: 275 2757 484 4090 Fragments arranged by size: 4090 2757 484 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 4409 4496 4580 5536 1353 Size: 911 2145 87 84 956 3423 Fragments arranged by size: 3423 2145 956 911 87 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2167 2486 3686 6193 6567 6713 Size: 1064 138 578 319 1200 2507 374 146 Cuts at: 6713 387 Size: 1280 Fragments arranged by size: 2507 1280 1200 1064 578 374 319 146 138 EcoICRI GAG'CTC Cuts at: 2167 3198 3592 4590 2167 Size: 1031 394 998 5183 Fragments arranged by size: 5183 1031 998 394 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2732 3014 3169 3368 3437 3516 Size: 393 599 501 282 155 199 69 79 Cuts at: 3516 3578 3620 3633 4601 4674 4697 4760 5669 Size: 62 42 13 968 73 23 63 909 Cuts at: 5669 6266 7053 7321 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 968 909 787 599 597 501 393 282 268 199 155 79 73 69 63 62 42 23 13 EcoNI CCTnn'n_nnAGG Cuts at: 3648 3648 Size: 7606 EcoO109I rG'GnC_Cy Cuts at: 2096 2191 2544 2545 2682 3534 3580 3653 4072 Size: 95 353 1 137 852 46 73 419 Cuts at: 4072 4140 4550 4551 4609 4728 4729 2096 Size: 68 410 1 58 119 1 4973 Fragments arranged by size: 4973 852 419 410 353 137 119 95 73 68 58 46 1 1 1 EcoRI G'AATT_C Cuts at: 4915 5293 4915 Size: 378 7228 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3380 3380 Size: 7606 FspI TGC'GCA Cuts at: 6112 6112 Size: 7606 HaeII r_GCGC'y Cuts at: 1678 2033 2079 2405 3825 6753 1678 Size: 355 46 326 1420 2928 2531 Fragments arranged by size: 2928 2531 1420 355 326 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 3506 3538 3868 Size: 32 135 32 899 32 1003 32 330 Cuts at: 3868 3900 4056 4088 6247 6279 6321 6353 1373 Size: 32 156 32 2159 32 42 32 2626 Fragments arranged by size: 2626 2159 1003 899 330 156 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4950 5196 669 Size: 988 281 3012 246 3079 Fragments arranged by size: 3079 3012 988 281 246 HindIII A'AGCT_T Cuts at: 748 4647 748 Size: 3899 3707 HpaI GTT'AAC Cuts at: 5196 5196 Size: 7606 KasI G'GCGC_C Cuts at: 2075 3821 2075 Size: 1746 5860 KpnI G_GTAC'C Cuts at: 4931 4931 Size: 7606 MfeI C'AATT_G Cuts at: 5205 5205 Size: 7606 MluI A'CGCG_T Cuts at: 2616 7482 2616 Size: 4866 2740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3172 3942 6720 6904 1305 Size: 1867 770 2778 184 2007 Fragments arranged by size: 2778 2007 1867 770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2759 10 Size: 54 1425 343 927 4857 Fragments arranged by size: 4857 1425 927 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2868 3510 3627 5564 5923 6082 518 Size: 648 1702 642 117 1937 359 159 2042 Fragments arranged by size: 2042 1937 1702 648 642 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2380 3055 3193 3304 3598 3909 4282 5022 Size: 774 675 138 111 294 311 373 740 Cuts at: 5022 5699 6847 7092 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 774 740 677 675 373 311 294 245 138 111 NaeI GCC'GGC Cuts at: 2071 2071 Size: 7606 NarI GG'CG_CC Cuts at: 2076 3822 2076 Size: 1746 5860 NcoI C'CATG_G Cuts at: 513 1065 2949 4314 4797 513 Size: 552 1884 1365 483 3322 Fragments arranged by size: 3322 1884 1365 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 7606 NgoMIV G'CCGG_C Cuts at: 2069 2069 Size: 7606 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7606 NotI GC'GGCC_GC Cuts at: 5053 5053 Size: 7606 NsiI A_TGCA'T Cuts at: 3345 3345 Size: 7606 NspI r_CATG'y Cuts at: 1132 1229 4964 6509 1132 Size: 97 3735 1545 2229 Fragments arranged by size: 3735 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2770 3538 4051 4192 4670 1348 Size: 1422 768 513 141 478 4284 Fragments arranged by size: 4284 1422 768 513 478 141 PciI A'CATG_T Cuts at: 6505 6505 Size: 7606 PflMI CCAn_nnn'nTGG Cuts at: 1108 2677 2890 3583 4612 4844 1108 Size: 1569 213 693 1029 232 3870 Fragments arranged by size: 3870 1569 1029 693 232 213 PfoI T'CCnGG_A Cuts at: 2060 2463 2958 3072 3414 4506 2060 Size: 403 495 114 342 1092 5160 Fragments arranged by size: 5160 1092 495 403 342 114 PmeI GTTT'AAAC Cuts at: 4910 4910 Size: 7606 PmlI CAC'GTG Cuts at: 4327 4903 4327 Size: 576 7030 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2294 2326 5675 5707 7188 7220 2294 Size: 32 3349 32 1481 32 2680 Fragments arranged by size: 3349 2680 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2191 2682 3580 4072 2191 Size: 491 898 492 5725 Fragments arranged by size: 5725 898 492 491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7606 PsiI TTA'TAA Cuts at: 2733 5176 2733 Size: 2443 5163 PspOMI G'GGCC_C Cuts at: 2096 2544 4550 4728 2096 Size: 448 2006 178 4974 Fragments arranged by size: 4974 2006 448 178 PstI C_TGCA'G Cuts at: 830 2370 2427 2571 2745 2784 4958 830 Size: 1540 57 144 174 39 2174 3478 Fragments arranged by size: 3478 2174 1540 174 144 57 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7606 PvuII CAG'CTG Cuts at: 2380 3055 3193 3304 3598 2380 Size: 675 138 111 294 6388 Fragments arranged by size: 6388 675 294 138 111 SacI G_AGCT'C Cuts at: 2169 3200 3594 4592 2169 Size: 1031 394 998 5183 Fragments arranged by size: 5183 1031 998 394 SacII CC_GC'GG Cuts at: 4283 4283 Size: 7606 SalI G'TCGA_C Cuts at: 1936 4948 1936 Size: 3012 4594 SanDI GG'GwC_CC Cuts at: 2682 2682 Size: 7606 SapI GCTCTTCn'nnn_ Cuts at: 4496 4580 4496 Size: 84 7522 SbfI CC_TGCA'GG Cuts at: 2571 4958 2571 Size: 2387 5219 ScaI AGT'ACT Cuts at: 3954 5854 7566 3954 Size: 1900 1712 3994 Fragments arranged by size: 3994 1900 1712 SfcI C'TryA_G Cuts at: 826 1045 2344 2366 2423 2567 2741 2780 2852 Size: 219 1299 22 57 144 174 39 72 Cuts at: 2852 4954 6089 6770 6961 826 Size: 2102 1135 681 191 1471 Fragments arranged by size: 2102 1471 1299 1135 681 219 191 174 144 72 57 39 22 SfiI GGCCn_nnn'nGGCC Cuts at: 3710 3710 Size: 7606 SfoI GGC'GCC Cuts at: 2077 3823 2077 Size: 1746 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7606 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7606 SmaI CCC'GGG Cuts at: 1872 2094 4933 1872 Size: 222 2839 4545 Fragments arranged by size: 4545 2839 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 5710 6611 6873 820 Size: 197 926 3767 901 262 1553 Fragments arranged by size: 3767 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5397 493 Size: 4904 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7606 SphI G_CATG'C Cuts at: 1132 4964 1132 Size: 3832 3774 SspI AAT'ATT Cuts at: 5 52 5530 5 Size: 47 5478 2081 Fragments arranged by size: 5478 2081 47 StuI AGG'CCT Cuts at: 1517 2831 3254 3380 3476 1517 Size: 1314 423 126 96 5647 Fragments arranged by size: 5647 1314 423 126 96 StyI C'CwwG_G Cuts at: 513 1065 2949 3387 4075 4314 4797 5045 513 Size: 552 1884 438 688 239 483 248 3074 Fragments arranged by size: 3074 1884 688 552 483 438 248 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5633 5650 5803 5988 6433 325 Size: 1255 4053 17 153 185 445 1498 Fragments arranged by size: 4053 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3952 4266 5852 Size: 274 80 33 51 1443 1975 314 1586 Cuts at: 5852 7564 96 Size: 1712 138 Fragments arranged by size: 1975 1712 1586 1443 314 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7566 7598 7566 Size: 32 7574 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7606 XbaI T'CTAG_A Cuts at: 4942 4942 Size: 7606 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 3316 3943 2767 Size: 549 627 6430 Fragments arranged by size: 6430 627 549 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7606 XmaI C'CCGG_G Cuts at: 1870 2092 4931 1870 Size: 222 2839 4545 Fragments arranged by size: 4545 2839 222 XmnI GAAnn'nnTTC Cuts at: 3501 4226 5735 3501 Size: 725 1509 5372 Fragments arranged by size: 5372 1509 725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5414 276 Size: 53 83 186 936 3880 2468 Fragments arranged by size: 3880 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI PacI PspXI PsrI RsrII SexAI SrfI SwaI