################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BcgI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1099 GTCTAC GT^[AC][GT]AC AcyI 1225 GGCGCC G[GA]^CG[CT]C AocI 181 CCTGAGG CC^T[ACGT]AGG AvaI 883 CCCGAG C^[CT]CG[GA]G BbeI 1225 GGCGCC GGCGC^C BbrPI 688 CACGTG CAC^GTG BbvCI 869 CCTCAGC CCTCAGC(-5/-2) BciVI 360 GTATCC GTATCC(6/5) BcnI 203 CCCGG CC^[GC]GG BlpI 54 GCTAAGC GC^T[ACGT]AGC BpmI 1013 CTCCAG CTGGAG(16/14) Bpu1102I 54 GCTAAGC GC^T[ACGT]AGC BsaAI 688 CACGTG [CT]AC^GT[GA] BsaHI 1225 GGCGCC G[GA]^CG[CT]C BsaWI 363 TCCGGT [AT]^CCGG[AT] BsmFI 888 GTCCC GGGAC(10/14) BsoBI 883 CCCGAG C^[CT]CG[GA]G BspHI 860 TCATGA T^CATGA BspMI 979 ACCTGC ACCTGC(4/8) BsrBI 727 GAGCGG CCGCTC(-3/-3) BssSI 1048 CACGAG CACGAG(-5/-1) BstEII 366 GGTGACC G^GT[ACGT]ACC BstPI 366 GGTGACC G^GT[ACGT]ACC BstYI 344 AGATCC [GA]^GATC[CT] Bsu36I 181 CCTGAGG CC^T[ACGT]AGG CelII 54 GCTAAGC GC^T[ACGT]AGC DraII 1008 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 1368 GACCGAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 181 CCTGAGG CC^T[ACGT]AGG EcoNI 705 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1008 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 366 GGTGACC G^GT[ACGT]ACC EcoT22I 855 ATGCAT ATGCA^T HgaI 780 GACGC GACGC(5/10) Hin1I 1225 GGCGCC G[GA]^CG[CT]C KasI 1225 GGCGCC G^GCGCC MflI 344 AGATCC [GA]^GATC[CT] NarI 1225 GGCGCC GG^CGCC NciI 203 CCCGG CC^[GC]GG NcoI 836 CCATGG C^CATGG NsiI 855 ATGCAT ATGCA^T NspI 926 GCATGT [GA]CATG^[CT] PmaCI 688 CACGTG CAC^GTG PmlI 688 CACGTG CAC^GTG PshAI 1092 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 860 TCATGA T^CATGA SapI 483 GAAGAGC GCTCTTC(1/4) SfoI 1225 GGCGCC GGC^GCC SmlI 1363 CTCAAG C^T[CT][GA]AG TaqI 725 TCGA T^CGA TthHB8I 725 TCGA T^CGA XhoII 344 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 198 GTAC GT^AC 936 GTAC AlwI 128 GATCC GGATC(4/5) 345 GATCC AspHI 375 GAGCTC G[AT]GC[AT]^C 796 GAGCTC AvaII 534 GGTCC G^G[AT]CC 1182 GGACC BglI 508 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 748 GCCAAGTTGGC BsiHKAI 375 GAGCTC G[AT]GC[AT]^C 796 GAGCTC BstAPI 171 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1433 GCACACCTTGC BstXI 236 CCAAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCATGACGCTGG HapII 204 CCGG C^CGG 364 CCGG HpaII 204 CCGG C^CGG 364 CCGG MspI 204 CCGG C^CGG 364 CCGG PflMI 286 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1173 CCAGACCCTGG PstI 226 CTGCAG CTGCA^G 1257 CTGCAG PvuII 811 CAGCTG CAG^CTG 985 CAGCTG RsaI 198 GTAC GT^AC 936 GTAC SacI 375 GAGCTC GAGCT^C 796 GAGCTC StuI 1274 AGGCCT AGG^CCT 1331 AGGCCT Van91I 286 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1173 CCAGACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AlwNI BalI BanI BanII BbvI BmyI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI XcmI