################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BanI BbeI BclI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI HindIII HinfI HpaI KasI KpnI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 484 GGCGTC G[GA]^CG[CT]C AlwI 995 GGATC GGATC(4/5) AlwNI 541 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG BbrPI 700 CACGTG CAC^GTG BbsI 796 GTCTTC GAAGAC(2/6) BbvCI 603 CCTCAGC CCTCAGC(-5/-2) BciVI 222 GGATAC GTATCC(6/5) BfaI 301 CTAG C^TAG BpuAI 796 GTCTTC GAAGAC(2/6) BsaAI 700 CACGTG [CT]AC^GT[GA] BsaHI 484 GGCGTC G[GA]^CG[CT]C BsgI 235 CTGCAC GTGCAG(16/14) BsrDI 870 CATTGC GCAATG(2/0) BsrI 567 CCAGT ACTGG(1/-1) BssSI 474 CTCGTG CACGAG(-5/-1) BstF5I 123 CATCC GGATG(2/0) BstXI 875 CCACCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 996 GATC ^GATC EarI 655 CTCTTC CTCTTC(1/4) FokI 123 CATCC GGATG(9/13) FseI 507 GGCCGGCC GGCCGG^CC Hin1I 484 GGCGTC G[GA]^CG[CT]C HincII 892 GTCAAC GT[CT]^[GA]AC HindII 892 GTCAAC GT[CT]^[GA]AC Ksp632I 655 CTCTTC CTCTTC(1/4) MaeI 301 CTAG C^TAG MaeIII 649 GTCAC ^GT[ACGT]AC MboI 996 GATC ^GATC NaeI 508 GCCGGC GCC^GGC NdeII 996 GATC ^GATC NgoMIV 508 GCCGGC G^CCGGC NlaIII 396 CATG CATG^ NotI 305 GCGGCCGC GC^GGCCGC PaeR7I 433 CTCGAG C^TCGAG PmaCI 700 CACGTG CAC^GTG PmlI 700 CACGTG CAC^GTG PpuMI 98 GGGACCT [GA]G^G[AT]CC[CT] Sau3AI 996 GATC ^GATC SmlI 433 CTCGAG C^T[CT][GA]AG Tsp45I 649 GTCAC ^GT[GC]AC XhoI 433 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 118 GTCTAC GT^[AC][GT]AC 526 GTCTAC AspHI 265 GTGCTC G[AT]GC[AT]^C 750 GAGCAC AviII 449 TGCGCA TGC^GCA 728 TGCGCA BlpI 339 GCTCAGC GC^T[ACGT]AGC 1001 GCTCAGC Bpu1102I 339 GCTCAGC GC^T[ACGT]AGC 1001 GCTCAGC BsiHKAI 265 GTGCTC G[AT]GC[AT]^C 750 GAGCAC BspMI 101 ACCTGC ACCTGC(4/8) 877 ACCTGC BsrBI 910 CCGCTC CCGCTC(-3/-3) 933 CCGCTC BsrFI 508 GCCGGC [GA]^CCGG[CT] 976 ACCGGC BssHII 377 GCGCGC G^CGCGC 464 GCGCGC BstAPI 362 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 540 GCAGCCCCTGC CelII 339 GCTCAGC GC^T[ACGT]AGC 1001 GCTCAGC Cfr10I 508 GCCGGC [GA]^CCGG[CT] 976 ACCGGC FspI 449 TGCGCA TGC^GCA 728 TGCGCA HgaI 485 GCGTC GACGC(5/10) 810 GCGTC KspI 208 CCGCGG CCGC^GG 287 CCGCGG MslI 396 CATGAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 753 CACGTTTGTG PvuII 248 CAGCTG CAG^CTG 897 CAGCTG SacII 208 CCGCGG CCGC^GG 287 CCGCGG SfaNI 671 GCATC GCATC(5/9) 731 GCATC SmaI 30 CCCGGG CCC^GGG 947 CCCGGG TaqI 434 TCGA T^CGA 746 TCGA TthHB8I 434 TCGA T^CGA 746 TCGA XmaCI 30 CCCGGG C^CCGGG 947 CCCGGG XmaI 30 CCCGGG C^CCGGG 947 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AluI ApaI AvaI AvaII BanII BbvI BcgI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI TspRI