################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 921 CCGC CCGC(-3/-1) AlwNI 1773 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1447 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1117 CCCGAG C^[CT]CG[GA]G BalI 447 TGGCCA TGG^CCA BamHI 1216 GGATCC G^GATCC BbsI 1139 GTCTTC GAAGAC(2/6) BcnI 1495 CCGGG CC^[GC]GG BfaI 1145 CTAG C^TAG BglII 338 AGATCT A^GATCT BpuAI 1139 GTCTTC GAAGAC(2/6) BsmI 1042 GAATGC GAATGC(1/-1) BsoBI 1117 CCCGAG C^[CT]CG[GA]G BspMI 641 ACCTGC ACCTGC(4/8) BsrDI 710 GCAATG GCAATG(2/0) BstAPI 1538 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 637 GGTTACC G^GT[ACGT]ACC BstPI 637 GGTTACC G^GT[ACGT]ACC BstXI 768 CCAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 1539 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 447 TGGCCA [CT]^GGCC[GA] EcoO65I 637 GGTTACC G^GT[ACGT]ACC EcoRI 1348 GAATTC G^AATTC HapII 1495 CCGG C^CGG HgaI 1572 GACGC GACGC(5/10) HincII 229 GTCAAC GT[CT]^[GA]AC HindII 229 GTCAAC GT[CT]^[GA]AC HpaII 1495 CCGG C^CGG MaeI 1145 CTAG C^TAG MaeII 892 ACGT A^CGT MluNI 447 TGGCCA TGG^CCA MscI 447 TGGCCA TGG^CCA MseI 47 TTAA T^TAA MslI 30 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1495 CCGG C^CGG NciI 1495 CCGGG CC^[GC]GG NcoI 787 CCATGG C^CATGG PshAI 1613 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 137 ACCTGGT A^CC[AT]GGT Tru9I 47 TTAA T^TAA XmnI 1447 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1348 GAATTC [GA]^AATT[CT] 1759 AAATTT Alw44I 1098 GTGCAC G^TGCAC 1422 GTGCAC ApaLI 1098 GTGCAC G^TGCAC 1422 GTGCAC ApoI 1348 GAATTC [GA]^AATT[CT] 1759 AAATTT AvaII 1027 GGACC G^G[AT]CC 1172 GGTCC BsaBI 213 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 339 GATCTACATC BsaI 466 GAGACC GGTCTC(1/5) 658 GAGACC BseRI 1723 CTCCTC GAGGAG(10/8) 1384 GAGGAG BsgI 387 GTGCAG GTGCAG(16/14) 948 GTGCAG BsmAI 466 GAGAC GTCTC(1/5) 658 GAGAC Bsp1407I 538 TGTACA T^GTACA 802 TGTACA BspLU11I 1270 ACATGT A^CATGT 1597 ACATGT BsrGI 538 TGTACA T^GTACA 802 TGTACA BstYI 338 AGATCT [GA]^GATC[CT] 1216 GGATCC EarI 160 GAAGAG CTCTTC(1/4) 1447 GAAGAG EcoT14I 787 CCATGG C^C[AT][AT]GG 1667 CCTTGG Ksp632I 160 GAAGAG CTCTTC(1/4) 1447 GAAGAG MamI 213 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 339 GATCTACATC MflI 338 AGATCT [GA]^GATC[CT] 1216 GGATCC PflMI 281 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 742 CCAGCCAATGG SspBI 538 TGTACA T^GTACA 802 TGTACA StyI 787 CCATGG C^C[AT][AT]GG 1667 CCTTGG Van91I 281 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 742 CCAGCCAATGG XhoII 338 AGATCT [GA]^GATC[CT] 1216 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AfaI AflIII AluI AlwI AspHI BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI TseI Tsp45I Tsp509I TspRI XcmI