(Circular) (Six-base) MAPSORT of: pFN21AE2027.seq Check: 9854 from: 1 to: 6535 pFN21AE2027 6535 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4345 278 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3878 1937 Size: 1941 4594 Acc65I G'GTAC_C Cuts at: 3856 3856 Size: 6535 AclI AA'CG_TT Cuts at: 1563 4662 5035 1563 Size: 3099 373 3063 Fragments arranged by size: 3099 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2494 2587 2840 3364 3588 4598 5982 6250 Size: 862 93 253 524 224 1010 1384 268 Cuts at: 6250 1632 Size: 1917 Fragments arranged by size: 1917 1384 1010 862 524 268 253 224 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6338 AflIII A'CryG_T Cuts at: 1203 2897 3275 3602 5434 6411 1203 Size: 1694 378 327 1832 977 1327 Fragments arranged by size: 1832 1694 1327 977 378 327 AgeI A'CCGG_T Cuts at: 5366 5366 Size: 6535 AhdI GACnn_n'nnGTC Cuts at: 5264 5264 Size: 6535 AleI CACnn'nnGTG Cuts at: 1166 2039 1166 Size: 873 5662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6535 AlwNI CAG_nnn'CTG Cuts at: 3783 4278 5850 3783 Size: 495 1572 4468 Fragments arranged by size: 4468 1572 495 ApaLI G'TGCA_C Cuts at: 3103 3427 4592 5748 3103 Size: 324 1165 1156 3890 Fragments arranged by size: 3890 1165 1156 324 ApoI r'AATT_y Cuts at: 1493 3353 3764 3844 4070 4222 6331 1493 Size: 1860 411 80 226 152 2109 1697 Fragments arranged by size: 2109 1860 1697 411 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5089 160 Size: 872 4057 1606 Fragments arranged by size: 4057 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 AvaI C'yCGr_G Cuts at: 1870 1943 3122 3860 1870 Size: 73 1179 738 4545 Fragments arranged by size: 4545 1179 738 73 BamHI G'GATC_C Cuts at: 3221 3865 4232 3221 Size: 644 367 5524 Fragments arranged by size: 5524 644 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3856 5311 618 Size: 325 217 627 2069 1455 1842 Fragments arranged by size: 2069 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2299 2848 3534 3854 1391 Size: 908 549 686 320 4072 Fragments arranged by size: 4072 908 686 549 320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3137 928 Size: 971 1238 4326 Fragments arranged by size: 4326 1238 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4726 4760 1903 Size: 34 2789 34 3678 Fragments arranged by size: 3678 2789 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4427 5637 4427 Size: 1210 5325 BclI T'GATC_A Cuts at: 1548 1548 Size: 6535 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5146 136 Size: 107 122 71 631 605 3474 1525 Fragments arranged by size: 3474 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2343 6530 2343 Size: 4187 2348 BlpI GC'TnA_GC Cuts at: 3952 3952 Size: 6535 Bme1580I G_kGCm'C Cuts at: 1790 3107 3431 4596 5752 1790 Size: 1317 324 1165 1156 2573 Fragments arranged by size: 2573 1317 1165 1156 324 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6535 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 2603 3077 Size: 416 53 83 399 24 885 301 474 Cuts at: 3077 4266 5224 442 Size: 1189 958 1753 Fragments arranged by size: 1753 1189 958 885 474 416 399 301 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6535 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3351 3351 Size: 6535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2706 2991 5195 1239 Size: 1044 423 285 2204 2579 Fragments arranged by size: 2579 2204 1044 423 285 Bpu10I CC'TnA_GC Cuts at: 2562 2562 Size: 6535 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 3042 3443 4660 5525 5823 2504 Size: 538 401 1217 865 298 3216 Fragments arranged by size: 3216 1217 865 538 401 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2465 2657 5198 882 Size: 617 966 192 2541 2219 Fragments arranged by size: 2541 2219 966 617 192 BsaAI yAC'GTr Cuts at: 493 1204 4326 493 Size: 711 3122 2702 Fragments arranged by size: 3122 2702 711 BsaBI GATnn'nnATC Cuts at: 2222 2348 6529 2222 Size: 126 4181 2228 Fragments arranged by size: 4181 2228 126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4342 4724 5359 275 Size: 53 83 186 936 2809 382 635 1451 Fragments arranged by size: 2809 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4968 5366 5640 5787 6198 4968 Size: 398 274 147 411 5305 Fragments arranged by size: 5305 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2168 2198 3521 Size: 30 165 30 663 30 72 30 1323 Cuts at: 3521 3551 3690 3711 3720 3741 1178 Size: 30 139 21 9 21 3972 Fragments arranged by size: 3972 1323 663 165 139 72 30 30 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3404 3719 1186 Size: 2218 315 4002 Fragments arranged by size: 4002 2218 315 BseYI C'CCAG_C Cuts at: 5738 6234 5738 Size: 496 6039 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2413 2974 1163 Size: 573 677 561 4724 Fragments arranged by size: 4724 677 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3985 4746 5774 1540 Size: 275 186 1984 761 1028 2301 Fragments arranged by size: 2301 1984 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2701 3107 3337 3431 4596 4681 5752 2701 Size: 406 230 94 1165 85 1071 3484 Fragments arranged by size: 3484 1165 1071 406 230 94 85 BsmI GAATG_Cn' Cuts at: 3053 4046 4139 3053 Size: 993 93 5449 Fragments arranged by size: 5449 993 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2299 2701 2848 3107 3337 3431 3534 Size: 399 509 402 147 259 230 94 103 Cuts at: 3534 3854 4596 4681 5752 1391 Size: 320 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 402 399 320 259 230 147 103 94 85 BspEI T'CCGG_A Cuts at: 6198 6198 Size: 6535 BspHI T'CATG_A Cuts at: 4424 6154 4424 Size: 1730 4805 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 3890 844 Size: 1811 1235 3489 Fragments arranged by size: 3489 1811 1235 BsrBI CCG'CTC Cuts at: 1394 1823 4422 1394 Size: 429 2599 3507 Fragments arranged by size: 3507 2599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2722 5030 5204 65 Size: 1183 1474 2308 174 1396 Fragments arranged by size: 2308 1474 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5179 5366 1700 Size: 110 3369 187 2869 Fragments arranged by size: 3369 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2543 2807 96 Size: 2447 264 3824 Fragments arranged by size: 3824 2447 264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6535 BssSI C'ACGA_G Cuts at: 4595 5607 4595 Size: 1012 5523 BstAPI GCAn_nnn'nTGC Cuts at: 794 3549 794 Size: 2755 3780 BstBI TT'CG_AA Cuts at: 5339 5339 Size: 6535 BstEII G'GTnAC_C Cuts at: 1184 2642 1184 Size: 1458 5077 BstXI CCAn_nnnn'nTGG Cuts at: 2780 2780 Size: 6535 BstYI r'GATC_y Cuts at: 1972 2343 3221 3865 4232 4617 4634 6086 6172 Size: 371 878 644 367 385 17 1452 86 Cuts at: 6172 6184 6530 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 878 644 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2085 2455 2792 513 Size: 552 1020 370 337 4256 Fragments arranged by size: 4256 1020 552 370 337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3309 487 Size: 683 56 2083 3713 Fragments arranged by size: 3713 2083 683 56 BtsI GCAGTG_nn' Cuts at: 2455 2678 3071 3529 4051 4845 4865 2455 Size: 223 393 458 522 794 20 4125 Fragments arranged by size: 4125 794 522 458 393 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6535 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3047 3082 529 Size: 35 2483 35 3982 Fragments arranged by size: 3982 2483 35 35 DraI TTT'AAA Cuts at: 3839 3839 Size: 6535 DraIII CAC_nnn'GTG Cuts at: 3549 3549 Size: 6535 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5542 809 Size: 507 4226 1802 Fragments arranged by size: 4226 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2452 3982 4871 Size: 54 1425 50 275 18 622 1530 889 Cuts at: 4871 6500 8 Size: 1629 43 Fragments arranged by size: 1629 1530 1425 889 622 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3982 1537 Size: 275 2170 4090 Fragments arranged by size: 4090 2170 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 3447 4465 1353 Size: 807 1287 1018 3423 Fragments arranged by size: 3423 1287 1018 807 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2915 5122 5496 5642 387 Size: 1064 138 1326 2207 374 146 1280 Fragments arranged by size: 2207 1326 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2494 2587 2706 2840 2991 3364 Size: 393 651 211 93 119 134 151 373 Cuts at: 3364 3588 4598 5195 5982 6250 1239 Size: 224 1010 597 787 268 1524 Fragments arranged by size: 1524 1010 787 651 597 393 373 268 224 211 151 134 119 93 EcoRI G'AATT_C Cuts at: 3353 3844 4222 3353 Size: 491 378 5666 Fragments arranged by size: 5666 491 378 FspI TGC'GCA Cuts at: 5041 5041 Size: 6535 HaeII r_GCGC'y Cuts at: 1678 5682 1678 Size: 4004 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3416 3448 3564 3596 3690 Size: 32 135 32 1844 32 116 32 94 Cuts at: 3690 3722 5176 5208 5250 5282 1373 Size: 32 1454 32 42 32 2626 Fragments arranged by size: 2626 1844 1454 135 116 94 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 3879 4125 669 Size: 988 281 298 1643 246 3079 Fragments arranged by size: 3079 1643 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 2405 2785 3093 748 Size: 1657 380 308 4190 Fragments arranged by size: 4190 1657 380 308 HpaI GTT'AAC Cuts at: 4125 4125 Size: 6535 KpnI G_GTAC'C Cuts at: 3860 3860 Size: 6535 MfeI C'AATT_G Cuts at: 4134 4134 Size: 6535 MluI A'CGCG_T Cuts at: 6411 6411 Size: 6535 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3141 3687 5649 5833 1305 Size: 1836 546 1962 184 2007 Fragments arranged by size: 2007 1962 1836 546 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2454 10 Size: 54 1425 343 622 4091 Fragments arranged by size: 4091 1425 622 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 4493 4852 5011 518 Size: 648 873 2454 359 159 2042 Fragments arranged by size: 2454 2042 873 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2426 2631 3183 3384 3783 3951 4628 5776 Size: 820 205 552 201 399 168 677 1148 Cuts at: 5776 6021 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 820 677 552 399 245 205 201 168 NcoI C'CATG_G Cuts at: 513 1065 2792 513 Size: 552 1727 4256 Fragments arranged by size: 4256 1727 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6535 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6535 NotI GC'GGCC_GC Cuts at: 3982 3982 Size: 6535 NspI r_CATG'y Cuts at: 1132 1229 2377 2916 3279 3606 3893 5438 1132 Size: 97 1148 539 363 327 287 1545 2229 Fragments arranged by size: 2229 1545 1148 539 363 327 287 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6535 PciI A'CATG_T Cuts at: 3275 3602 5434 3275 Size: 327 1832 4376 Fragments arranged by size: 4376 1832 327 PflMI CCAn_nnn'nTGG Cuts at: 1108 2292 2753 1108 Size: 1184 461 4890 Fragments arranged by size: 4890 1184 461 PfoI T'CCnGG_A Cuts at: 3499 3499 Size: 6535 PmeI GTTT'AAAC Cuts at: 3839 3839 Size: 6535 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4604 4636 6117 6149 4604 Size: 32 1481 32 4990 Fragments arranged by size: 4990 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3622 1613 Size: 2009 4526 PsiI TTA'TAA Cuts at: 4105 4105 Size: 6535 PstI C_TGCA'G Cuts at: 830 2450 3524 3595 3887 830 Size: 1620 1074 71 292 3478 Fragments arranged by size: 3478 1620 1074 292 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6535 PvuII CAG'CTG Cuts at: 2426 2631 3183 3384 3783 2426 Size: 205 552 201 399 5178 Fragments arranged by size: 5178 552 399 205 201 SalI G'TCGA_C Cuts at: 1936 3877 1936 Size: 1941 4594 SbfI CC_TGCA'GG Cuts at: 3887 3887 Size: 6535 ScaI AGT'ACT Cuts at: 4783 6495 4783 Size: 1712 4823 SexAI A'CCwGG_T Cuts at: 2142 2142 Size: 6535 SfcI C'TryA_G Cuts at: 826 1045 2446 3520 3591 3883 5018 5699 5890 Size: 219 1401 1074 71 292 1135 681 191 Cuts at: 5890 826 Size: 1471 Fragments arranged by size: 1471 1401 1135 1074 681 292 219 191 71 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6535 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6535 SmaI CCC'GGG Cuts at: 1872 3862 1872 Size: 1990 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 3057 3422 4639 5540 5802 Size: 197 926 576 538 365 1217 901 262 Cuts at: 5802 820 Size: 1553 Fragments arranged by size: 1553 1217 926 901 576 538 365 262 197 SnaBI TAC'GTA Cuts at: 493 4326 493 Size: 3833 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6535 SphI G_CATG'C Cuts at: 1132 3893 1132 Size: 2761 3774 SspI AAT'ATT Cuts at: 5 52 4459 5 Size: 47 4407 2081 Fragments arranged by size: 4407 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6535 StyI C'CwwG_G Cuts at: 513 1065 2792 3672 3974 513 Size: 552 1727 880 302 3074 Fragments arranged by size: 3074 1727 880 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2604 4562 4579 4732 4917 5362 325 Size: 1255 1024 1958 17 153 185 445 1498 Fragments arranged by size: 1958 1498 1255 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2543 2807 4781 Size: 274 80 33 51 1443 566 264 1974 Cuts at: 4781 6493 96 Size: 1712 138 Fragments arranged by size: 1974 1712 1443 566 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3063 3095 3711 3743 6495 6527 3063 Size: 32 616 32 2752 32 3071 Fragments arranged by size: 3071 2752 616 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6535 XbaI T'CTAG_A Cuts at: 3871 3871 Size: 6535 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2211 2758 2936 2966 3670 3729 2211 Size: 547 178 30 704 59 5017 Fragments arranged by size: 5017 704 547 178 59 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6535 XmaI C'CCGG_G Cuts at: 1870 3860 1870 Size: 1990 4545 XmnI GAAnn'nnTTC Cuts at: 3456 4664 3456 Size: 1208 5327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4343 276 Size: 53 83 186 936 2809 2468 Fragments arranged by size: 2809 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI