(Linear) (Six-base) MAPSORT of: KIEE2027.seq Check: 3810 from: 1 to: 1830 KIEE2027.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 582 835 1359 1583 1830 Size: 489 93 253 524 224 247 Fragments arranged by size: 524 489 253 247 224 93 AflIII A'CryG_T Cuts at: 0 892 1270 1597 1830 Size: 892 378 327 233 Fragments arranged by size: 892 378 327 233 AleI CACnn'nnGTG Cuts at: 0 34 1830 Size: 34 1796 AlwNI CAG_nnn'CTG Cuts at: 0 1778 1830 Size: 1778 52 ApaLI G'TGCA_C Cuts at: 0 1098 1422 1830 Size: 1098 324 408 Fragments arranged by size: 1098 408 324 ApoI r'AATT_y Cuts at: 0 1348 1759 1830 Size: 1348 411 71 Fragments arranged by size: 1348 411 71 AvaI C'yCGr_G Cuts at: 0 1117 1830 Size: 1117 713 BamHI G'GATC_C Cuts at: 0 1216 1830 Size: 1216 614 BanII G_rGCy'C Cuts at: 0 294 843 1529 1830 Size: 294 549 686 301 Fragments arranged by size: 686 549 301 294 BbsI GAAGACnn'nnnn_ Cuts at: 0 1132 1830 Size: 1132 698 BglII A'GATC_T Cuts at: 0 338 1830 Size: 338 1492 Bme1580I G_kGCm'C Cuts at: 0 1102 1426 1830 Size: 1102 324 404 Fragments arranged by size: 1102 404 324 BmrI ACTGGGnnnn_n' Cuts at: 0 297 598 1072 1830 Size: 297 301 474 758 Fragments arranged by size: 758 474 301 297 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1346 1830 Size: 1346 484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 701 986 1830 Size: 278 423 285 844 Fragments arranged by size: 844 423 285 278 Bpu10I CC'TnA_GC Cuts at: 0 557 1830 Size: 557 1273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 1037 1438 1830 Size: 499 538 401 392 Fragments arranged by size: 538 499 401 392 BsaI GGTCTCn'nnnn_ Cuts at: 0 460 652 1830 Size: 460 192 1178 Fragments arranged by size: 1178 460 192 BsaBI GATnn'nnATC Cuts at: 0 217 343 1830 Size: 217 126 1487 Fragments arranged by size: 1487 217 126 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 61 91 163 193 1516 1546 1685 1706 Size: 61 30 72 30 1323 30 139 21 Cuts at: 1706 1715 1736 1830 Size: 9 21 94 Fragments arranged by size: 1323 139 94 72 61 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1399 1714 1830 Size: 1399 315 116 Fragments arranged by size: 1399 315 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 408 969 1830 Size: 408 561 861 Fragments arranged by size: 861 561 408 BsiHKAI G_wGCw'C Cuts at: 0 696 1102 1332 1426 1830 Size: 696 406 230 94 404 Fragments arranged by size: 696 406 404 230 94 BsmI GAATG_Cn' Cuts at: 0 1048 1830 Size: 1048 782 Bsp1286I G_dGCh'C Cuts at: 0 294 696 843 1102 1332 1426 1529 1830 Size: 294 402 147 259 230 94 103 301 Fragments arranged by size: 402 301 294 259 230 147 103 94 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 650 1830 Size: 650 1180 BsrDI GCAATG_nn' Cuts at: 0 717 1830 Size: 717 1113 BsrGI T'GTAC_A Cuts at: 0 538 802 1830 Size: 538 264 1028 Fragments arranged by size: 1028 538 264 BstAPI GCAn_nnn'nTGC Cuts at: 0 1544 1830 Size: 1544 286 BstEII G'GTnAC_C Cuts at: 0 637 1830 Size: 637 1193 BstXI CCAn_nnnn'nTGG Cuts at: 0 775 1830 Size: 775 1055 BstYI r'GATC_y Cuts at: 0 338 1216 1830 Size: 338 878 614 Fragments arranged by size: 878 614 338 BtgI C'CryG_G Cuts at: 0 80 450 787 1830 Size: 80 370 337 1043 Fragments arranged by size: 1043 370 337 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1304 1830 Size: 1304 526 BtsI GCAGTG_nn' Cuts at: 0 450 673 1066 1524 1830 Size: 450 223 393 458 306 Fragments arranged by size: 458 450 393 306 223 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1042 1077 1830 Size: 1042 35 753 Fragments arranged by size: 1042 753 35 DraIII CAC_nnn'GTG Cuts at: 0 1544 1830 Size: 1544 286 EaeI y'GGCC_r Cuts at: 0 447 1830 Size: 447 1383 EarI CTCTTCn'nnn_ Cuts at: 0 155 1442 1830 Size: 155 1287 388 Fragments arranged by size: 1287 388 155 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 910 1830 Size: 910 920 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 278 489 582 701 835 986 1359 1583 Size: 278 211 93 119 134 151 373 224 Cuts at: 1583 1830 Size: 247 Fragments arranged by size: 373 278 247 224 211 151 134 119 93 EcoRI G'AATT_C Cuts at: 0 1348 1830 Size: 1348 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1411 1443 1559 1591 1685 1717 1830 Size: 1411 32 116 32 94 32 113 Fragments arranged by size: 1411 116 113 94 32 32 32 HincII GTy'rAC Cuts at: 0 231 1830 Size: 231 1599 HindIII A'AGCT_T Cuts at: 0 400 780 1088 1830 Size: 400 380 308 742 Fragments arranged by size: 742 400 380 308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1136 1682 1830 Size: 1136 546 148 Fragments arranged by size: 1136 546 148 MscI TGG'CCA Cuts at: 0 449 1830 Size: 449 1381 MslI CAynn'nnrTG Cuts at: 0 34 1830 Size: 34 1796 MspA1I CmG'CkG Cuts at: 0 421 626 1178 1379 1778 1830 Size: 421 205 552 201 399 52 Fragments arranged by size: 552 421 399 205 201 52 NcoI C'CATG_G Cuts at: 0 787 1830 Size: 787 1043 NspI r_CATG'y Cuts at: 0 372 911 1274 1601 1830 Size: 372 539 363 327 229 Fragments arranged by size: 539 372 363 327 229 PciI A'CATG_T Cuts at: 0 1270 1597 1830 Size: 1270 327 233 Fragments arranged by size: 1270 327 233 PflMI CCAn_nnn'nTGG Cuts at: 0 287 748 1830 Size: 287 461 1082 Fragments arranged by size: 1082 461 287 PfoI T'CCnGG_A Cuts at: 0 1494 1830 Size: 1494 336 PshAI GACnn'nnGTC Cuts at: 0 1617 1830 Size: 1617 213 PstI C_TGCA'G Cuts at: 0 445 1519 1590 1830 Size: 445 1074 71 240 Fragments arranged by size: 1074 445 240 71 PvuII CAG'CTG Cuts at: 0 421 626 1178 1379 1778 1830 Size: 421 205 552 201 399 52 Fragments arranged by size: 552 421 399 205 201 52 SexAI A'CCwGG_T Cuts at: 0 137 1830 Size: 137 1693 SfcI C'TryA_G Cuts at: 0 441 1515 1586 1830 Size: 441 1074 71 244 Fragments arranged by size: 1074 441 244 71 SmlI C'TyrA_G Cuts at: 0 514 1052 1417 1830 Size: 514 538 365 413 Fragments arranged by size: 538 514 413 365 StyI C'CwwG_G Cuts at: 0 787 1667 1830 Size: 787 880 163 Fragments arranged by size: 880 787 163 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 599 1830 Size: 599 1231 TatI w'GTAC_w Cuts at: 0 538 802 1830 Size: 538 264 1028 Fragments arranged by size: 1028 538 264 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1058 1090 1706 1738 1830 Size: 1058 32 616 32 92 Fragments arranged by size: 1058 616 92 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 206 753 931 961 1665 1724 1830 Size: 206 547 178 30 704 59 106 Fragments arranged by size: 704 547 206 178 106 59 30 XmnI GAAnn'nnTTC Cuts at: 0 1451 1830 Size: 1451 379 Enzymes that do cut: AcuI AflIII AleI AlwNI ApaLI ApoI AvaI BamHI BanII BbsI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoRI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PciI PflMI PfoI PshAI PstI PvuII SexAI SfcI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI DrdI EagI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI