################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I TseI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AluI 324 AGCT AG^CT AlwNI 112 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 89 GGACC G^G[AT]CC BbrPI 222 CACGTG CAC^GTG BsaAI 222 CACGTG [CT]AC^GT[GA] BsaJI 24 CCTTGG C^C[ACGT][ACGT]GG BsmBI 233 GAGACG CGTCTC(1/5) BssKI 15 CCTGG ^CC[ACGT]GG BstF5I 12 CATCC GGATG(2/0) BstNI 15 CCTGG CC^[AT]GG DraII 88 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 88 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 15 CCTGG ^CC[AT]GG EcoT14I 24 CCTTGG C^C[AT][AT]GG FokI 12 CATCC GGATG(9/13) HphI 228 GGTGA GGTGA(8/7) MaeIII 328 GTGAC ^GT[ACGT]AC MslI 222 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 323 CAGCTG C[AC]G^C[GT]G MvaI 15 CCTGG CC^[AT]GG PflFI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 330 GACTC GAGTC(4/5) PmaCI 222 CACGTG CAC^GTG PmlI 222 CACGTG CAC^GTG PpuMI 88 AGGACCT [GA]G^G[AT]CC[CT] PvuII 323 CAGCTG CAG^CTG ScrFI 15 CCTGG CC^[ACGT]GG SfaNI 29 GCATC GCATC(5/9) SmlI 162 CTTGAG C^T[CT][GA]AG StyI 24 CCTTGG C^C[AT][AT]GG TaqI 313 TCGA T^CGA TfiI 131 GAATC G^A[AT]TC Tsp45I 328 GTGAC ^GT[GC]AC Tsp509I 174 AATT ^AATT TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 313 TCGA T^CGA XcmI 214 CCACAAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AlwI 211 GATCC GGATC(4/5) 282 GATCC BsrI 337 ACTGG ACTGG(1/-1) 121 CCAGT Cfr13I 18 GGCCC G^G[ACGT]CC 89 GGACC DpnII 211 GATC ^GATC 282 GATC HaeIII 18 GGCC GG^CC 193 GGCC HinfI 131 GAATC G^A[ACGT]TC 330 GACTC MboI 211 GATC ^GATC 282 GATC NdeII 211 GATC ^GATC 282 GATC NlaIII 249 CATG CATG^ 279 CATG NlaIV 157 GGCTCC GG[ACGT]^[ACGT]CC 301 GGAACC Sau3AI 211 GATC ^GATC 282 GATC Sau96I 18 GGCCC G^G[ACGT]CC 89 GGACC ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### BsmAI MaeII MnlI