(Linear) (Six-base) MAPSORT of: KIBB6221.seq Check: 5534 from: 1 to: 2010 KIBB6221.orf With 182 enzymes: SgfI * March 4, 2011 18:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1733 2010 Size: 1733 277 Acc65I G'GTAC_C Cuts at: 0 816 2010 Size: 816 1194 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 413 463 812 1063 1084 1385 1513 2010 Size: 413 50 349 251 21 301 128 497 Fragments arranged by size: 497 413 349 301 251 128 50 21 AlwNI CAG_nnn'CTG Cuts at: 0 121 2010 Size: 121 1889 ApoI r'AATT_y Cuts at: 0 509 519 1554 2010 Size: 509 10 1035 456 Fragments arranged by size: 1035 509 456 10 AvaI C'yCGr_G Cuts at: 0 48 2010 Size: 48 1962 AvrII C'CTAG_G Cuts at: 0 469 2010 Size: 469 1541 BamHI G'GATC_C Cuts at: 0 1871 2010 Size: 1871 139 BanI G'GyrC_C Cuts at: 0 25 198 816 1247 2010 Size: 25 173 618 431 763 Fragments arranged by size: 763 618 431 173 25 BanII G_rGCy'C Cuts at: 0 1231 1552 2010 Size: 1231 321 458 Fragments arranged by size: 1231 458 321 BbsI GAAGACnn'nnnn_ Cuts at: 0 362 2010 Size: 362 1648 BbvCI CC'TCA_GC Cuts at: 0 269 2010 Size: 269 1741 BclI T'GATC_A Cuts at: 0 1323 1811 2010 Size: 1323 488 199 Fragments arranged by size: 1323 488 199 BglI GCCn_nnn'nGGC Cuts at: 0 1969 2010 Size: 1969 41 BlpI GC'TnA_GC Cuts at: 0 755 2010 Size: 755 1255 BmrI ACTGGGnnnn_n' Cuts at: 0 835 2010 Size: 835 1175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 570 758 2010 Size: 266 304 188 1252 Fragments arranged by size: 1252 304 266 188 Bpu10I CC'TnA_GC Cuts at: 0 269 919 2010 Size: 269 650 1091 Fragments arranged by size: 1091 650 269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 276 2010 Size: 276 1734 BsaAI yAC'GTr Cuts at: 0 804 2010 Size: 804 1206 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 279 302 370 2010 Size: 279 23 68 1640 Fragments arranged by size: 1640 279 68 23 BseYI C'CCAG_C Cuts at: 0 13 775 2010 Size: 13 762 1235 Fragments arranged by size: 1235 762 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 659 2010 Size: 659 1351 BsiHKAI G_wGCw'C Cuts at: 0 1539 2010 Size: 1539 471 BsmBI CGTCTCn'nnnn_ Cuts at: 0 145 314 2010 Size: 145 169 1696 Fragments arranged by size: 1696 169 145 Bsp1286I G_dGCh'C Cuts at: 0 1231 1539 1552 2010 Size: 1231 308 13 458 Fragments arranged by size: 1231 458 308 13 BspHI T'CATG_A Cuts at: 0 1440 2010 Size: 1440 570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 681 1733 2010 Size: 681 1052 277 Fragments arranged by size: 1052 681 277 BsrDI GCAATG_nn' Cuts at: 0 1372 2010 Size: 1372 638 BsrFI r'CCGG_y Cuts at: 0 9 22 2010 Size: 9 13 1988 Fragments arranged by size: 1988 13 9 BsrGI T'GTAC_A Cuts at: 0 241 2010 Size: 241 1769 BstBI TT'CG_AA Cuts at: 0 1713 2010 Size: 1713 297 BstYI r'GATC_y Cuts at: 0 1871 2010 Size: 1871 139 Bsu36I CC'TnA_GG Cuts at: 0 353 2010 Size: 353 1657 BtgI C'CryG_G Cuts at: 0 29 2010 Size: 29 1981 DraIII CAC_nnn'GTG Cuts at: 0 807 2010 Size: 807 1203 EarI CTCTTCn'nnn_ Cuts at: 0 432 1122 2010 Size: 432 690 888 Fragments arranged by size: 888 690 432 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 413 463 570 758 812 1063 1084 Size: 266 147 50 107 188 54 251 21 Cuts at: 1084 1385 1513 2010 Size: 301 128 497 Fragments arranged by size: 497 301 266 251 188 147 128 107 54 50 21 EcoRI G'AATT_C Cuts at: 0 519 2010 Size: 519 1491 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 930 962 1491 1523 1836 1868 2010 Size: 930 32 529 32 313 32 142 Fragments arranged by size: 930 529 313 142 32 32 32 KpnI G_GTAC'C Cuts at: 0 820 2010 Size: 820 1190 MfeI C'AATT_G Cuts at: 0 133 1775 2010 Size: 133 1642 235 Fragments arranged by size: 1642 235 133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 341 1172 2010 Size: 341 831 838 Fragments arranged by size: 838 831 341 MslI CAynn'nnrTG Cuts at: 0 1466 2010 Size: 1466 544 MspA1I CmG'CkG Cuts at: 0 433 1074 1563 2010 Size: 433 641 489 447 Fragments arranged by size: 641 489 447 433 NaeI GCC'GGC Cuts at: 0 11 24 2010 Size: 11 13 1986 Fragments arranged by size: 1986 13 11 NcoI C'CATG_G Cuts at: 0 29 2010 Size: 29 1981 NgoMIV G'CCGG_C Cuts at: 0 9 22 2010 Size: 9 13 1988 Fragments arranged by size: 1988 13 9 NspI r_CATG'y Cuts at: 0 349 1191 2010 Size: 349 842 819 Fragments arranged by size: 842 819 349 PflMI CCAn_nnn'nTGG Cuts at: 0 847 2010 Size: 847 1163 PfoI T'CCnGG_A Cuts at: 0 1204 2010 Size: 1204 806 PmlI CAC'GTG Cuts at: 0 804 2010 Size: 804 1206 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1442 1474 2010 Size: 1442 32 536 Fragments arranged by size: 1442 536 32 PsiI TTA'TAA Cuts at: 0 1162 2010 Size: 1162 848 PvuII CAG'CTG Cuts at: 0 1074 1563 2010 Size: 1074 489 447 Fragments arranged by size: 1074 489 447 SfcI C'TryA_G Cuts at: 0 1314 2010 Size: 1314 696 SmaI CCC'GGG Cuts at: 0 50 2010 Size: 50 1960 SmlI C'TyrA_G Cuts at: 0 291 2010 Size: 291 1719 SpeI A'CTAG_T Cuts at: 0 1591 2010 Size: 1591 419 StyI C'CwwG_G Cuts at: 0 29 202 469 2010 Size: 29 173 267 1541 Fragments arranged by size: 1541 267 173 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 305 582 693 1659 1759 2010 Size: 305 277 111 966 100 251 Fragments arranged by size: 966 305 277 251 111 100 TatI w'GTAC_w Cuts at: 0 241 2010 Size: 241 1769 Tth111I GACn'n_nGTC Cuts at: 0 1485 2010 Size: 1485 525 XmaI C'CCGG_G Cuts at: 0 48 2010 Size: 48 1962 XmnI GAAnn'nnTTC Cuts at: 0 855 2010 Size: 855 1155 Enzymes that do cut: AarI Acc65I AcuI AlwNI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BclI BglI BlpI BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I BtgI DraIII EarI Eco57MI EcoRI Hin4I KpnI MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PpiI PsiI PvuII SfcI SmaI SmlI SpeI StyI TaqII TatI Tth111I XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI BbeI BcgI BciVI BfrBI BglII Bme1580I BmgBI BmtI BplI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BsrBI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MluI MscI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI ZraI