(Circular) (Six-base) MAPSORT of: pFN21AE5145.seq Check: 994 from: 1 to: 5005 pFN21AE5145 5005 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2057 Size: 5005 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2815 278 Size: 53 83 186 936 1279 2468 Fragments arranged by size: 2468 1279 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2348 1937 Size: 411 4594 Acc65I G'GTAC_C Cuts at: 2326 2326 Size: 5005 AclI AA'CG_TT Cuts at: 1563 3132 3505 1563 Size: 1569 373 3063 Fragments arranged by size: 3063 1569 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 4452 4720 1632 Size: 1436 1384 268 1917 Fragments arranged by size: 1917 1436 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4808 AflIII A'CryG_T Cuts at: 1203 3904 4881 1203 Size: 2701 977 1327 Fragments arranged by size: 2701 1327 977 AgeI A'CCGG_T Cuts at: 3836 3836 Size: 5005 AhdI GACnn_n'nnGTC Cuts at: 3734 3734 Size: 5005 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5005 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5005 AlwNI CAG_nnn'CTG Cuts at: 2748 4320 2748 Size: 1572 3433 ApaLI G'TGCA_C Cuts at: 3062 4218 3062 Size: 1156 3849 ApoI r'AATT_y Cuts at: 1493 2314 2540 2692 4801 1493 Size: 821 226 152 2109 1697 Fragments arranged by size: 2109 1697 821 226 152 AseI AT'TA_AT Cuts at: 160 1032 3559 160 Size: 872 2527 1606 Fragments arranged by size: 2527 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5005 AvaI C'yCGr_G Cuts at: 1870 1943 2330 1870 Size: 73 387 4545 Fragments arranged by size: 4545 387 73 BamHI G'GATC_C Cuts at: 2197 2335 2702 2197 Size: 138 367 4500 Fragments arranged by size: 4500 367 138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 3781 618 Size: 325 217 627 539 1455 1842 Fragments arranged by size: 1842 1455 627 539 325 217 BanII G_rGCy'C Cuts at: 1391 2014 2182 2211 2232 2324 1391 Size: 623 168 29 21 92 4072 Fragments arranged by size: 4072 623 168 92 29 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4034 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3196 3230 1903 Size: 34 1259 34 3678 Fragments arranged by size: 3678 1259 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2897 4107 2897 Size: 1210 3795 BclI T'GATC_A Cuts at: 1548 1548 Size: 5005 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 3616 136 Size: 107 122 71 631 605 361 1583 1525 Fragments arranged by size: 1583 1525 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 5000 5000 Size: 5005 BlpI GC'TnA_GC Cuts at: 2422 2422 Size: 5005 Bme1580I G_kGCm'C Cuts at: 1790 3066 4222 1790 Size: 1276 1156 2573 Fragments arranged by size: 2573 1276 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5005 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 2736 3694 Size: 416 53 83 399 24 760 559 958 Cuts at: 3694 442 Size: 1753 Fragments arranged by size: 1753 958 760 559 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5005 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 3665 1239 Size: 986 1440 2579 Fragments arranged by size: 2579 1440 986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3130 3995 4293 3130 Size: 865 298 3842 Fragments arranged by size: 3842 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 3668 882 Size: 617 641 1528 2219 Fragments arranged by size: 2219 1528 641 617 BsaAI yAC'GTr Cuts at: 493 1204 2796 493 Size: 711 1592 2702 Fragments arranged by size: 2702 1592 711 BsaBI GATnn'nnATC Cuts at: 4999 4999 Size: 5005 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2812 3194 3829 275 Size: 53 83 186 936 1279 382 635 1451 Fragments arranged by size: 1451 1279 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2236 3438 3836 4110 4257 4668 2236 Size: 1202 398 274 147 411 2573 Fragments arranged by size: 2573 1202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2169 2199 1178 Size: 30 165 30 766 30 3984 Fragments arranged by size: 3984 766 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 1186 Size: 826 4179 BseYI C'CCAG_C Cuts at: 2078 2173 4208 4704 2078 Size: 95 2035 496 2379 Fragments arranged by size: 2379 2035 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2106 2124 1163 Size: 573 370 18 4044 Fragments arranged by size: 4044 573 370 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2304 2455 3216 4244 1540 Size: 275 186 303 151 761 1028 2301 Fragments arranged by size: 2301 1028 761 303 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2232 3066 3151 4222 2232 Size: 834 85 1071 3015 Fragments arranged by size: 3015 1071 834 85 BsmI GAATG_Cn' Cuts at: 2516 2609 2516 Size: 93 4912 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2182 2211 2232 2324 3066 3151 Size: 399 224 168 29 21 92 742 85 Cuts at: 3151 4222 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 224 168 92 85 29 21 BspEI T'CCGG_A Cuts at: 2236 4668 2236 Size: 2432 2573 BspHI T'CATG_A Cuts at: 2894 4624 2894 Size: 1730 3275 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2360 844 Size: 1213 303 3489 Fragments arranged by size: 3489 1213 303 BsrBI CCG'CTC Cuts at: 1394 1823 2892 1394 Size: 429 1069 3507 Fragments arranged by size: 3507 1069 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3500 3674 65 Size: 1183 2252 174 1396 Fragments arranged by size: 2252 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3649 3836 1700 Size: 110 1839 187 2869 Fragments arranged by size: 2869 1839 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5005 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5005 BssSI C'ACGA_G Cuts at: 3065 4077 3065 Size: 1012 3993 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5005 BstBI TT'CG_AA Cuts at: 3809 3809 Size: 5005 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5005 BstYI r'GATC_y Cuts at: 1972 2197 2335 2702 3087 3104 4556 4642 4654 Size: 225 138 367 385 17 1452 86 12 Cuts at: 4654 5000 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 225 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2038 487 Size: 683 56 812 3454 Fragments arranged by size: 3454 812 683 56 BtsI GCAGTG_nn' Cuts at: 2521 3315 3335 2521 Size: 794 20 4191 Fragments arranged by size: 4191 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5005 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4970 DraI TTT'AAA Cuts at: 2309 2309 Size: 5005 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4012 809 Size: 507 2696 1802 Fragments arranged by size: 2696 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2059 2452 Size: 54 1425 50 275 18 210 19 393 Cuts at: 2452 3341 4970 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 393 275 210 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2452 1537 Size: 275 640 4090 Fragments arranged by size: 4090 640 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2935 1353 Size: 785 797 3423 Fragments arranged by size: 3423 797 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3592 3966 4112 387 Size: 1064 138 2003 374 146 1280 Fragments arranged by size: 2003 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2230 2230 Size: 5005 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 3068 3665 4452 4720 1239 Size: 393 593 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 597 593 393 268 EcoRI G'AATT_C Cuts at: 2314 2692 2314 Size: 378 4627 FspI TGC'GCA Cuts at: 3511 3511 Size: 5005 HaeII r_GCGC'y Cuts at: 1678 2158 2290 4152 1678 Size: 480 132 1862 2531 Fragments arranged by size: 2531 1862 480 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2169 2201 3646 3678 3720 Size: 32 135 32 597 32 1445 32 42 Cuts at: 3720 3752 1373 Size: 32 2626 Fragments arranged by size: 2626 1445 597 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2349 2595 669 Size: 988 281 411 246 3079 Fragments arranged by size: 3079 988 411 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5005 HpaI GTT'AAC Cuts at: 2595 2595 Size: 5005 KpnI G_GTAC'C Cuts at: 2330 2330 Size: 5005 MfeI C'AATT_G Cuts at: 2604 2604 Size: 5005 MluI A'CGCG_T Cuts at: 4881 4881 Size: 5005 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4119 4303 1305 Size: 2814 184 2007 Fragments arranged by size: 2814 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2061 10 Size: 54 1425 343 229 2954 Fragments arranged by size: 2954 1425 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 2963 3322 3481 518 Size: 648 1797 359 159 2042 Fragments arranged by size: 2042 1797 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2082 2421 3098 4246 4491 1606 Size: 476 339 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 476 339 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4453 NdeI CA'TA_TG Cuts at: 387 387 Size: 5005 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5005 NotI GC'GGCC_GC Cuts at: 2452 2452 Size: 5005 NspI r_CATG'y Cuts at: 1132 1229 2363 3908 1132 Size: 97 1134 1545 2229 Fragments arranged by size: 2229 1545 1134 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5005 PciI A'CATG_T Cuts at: 3904 3904 Size: 5005 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5005 PmeI GTTT'AAAC Cuts at: 2309 2309 Size: 5005 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3074 3106 4587 4619 3074 Size: 32 1481 32 3460 Fragments arranged by size: 3460 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5005 PsiI TTA'TAA Cuts at: 2575 2575 Size: 5005 PstI C_TGCA'G Cuts at: 830 2357 830 Size: 1527 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5005 SacI G_AGCT'C Cuts at: 2232 2232 Size: 5005 SalI G'TCGA_C Cuts at: 1936 2347 1936 Size: 411 4594 SbfI CC_TGCA'GG Cuts at: 2357 2357 Size: 5005 ScaI AGT'ACT Cuts at: 3253 4965 3253 Size: 1712 3293 SfcI C'TryA_G Cuts at: 826 1045 2353 3488 4169 4360 826 Size: 219 1308 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5005 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5005 SmaI CCC'GGG Cuts at: 1872 2332 1872 Size: 460 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3109 4010 4272 820 Size: 197 926 1166 901 262 1553 Fragments arranged by size: 1553 1166 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2796 493 Size: 2303 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5005 SphI G_CATG'C Cuts at: 1132 2363 1132 Size: 1231 3774 SspI AAT'ATT Cuts at: 5 52 2929 5 Size: 47 2877 2081 Fragments arranged by size: 2877 2081 47 StuI AGG'CCT Cuts at: 1517 2279 1517 Size: 762 4243 StyI C'CwwG_G Cuts at: 513 1065 2444 513 Size: 552 1379 3074 Fragments arranged by size: 3074 1379 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3032 3049 3202 3387 3832 325 Size: 1255 1452 17 153 185 445 1498 Fragments arranged by size: 1498 1452 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3251 4963 96 Size: 274 80 33 51 1443 1274 1712 138 Fragments arranged by size: 1712 1443 1274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4965 4997 4965 Size: 32 4973 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5005 XbaI T'CTAG_A Cuts at: 2341 2341 Size: 5005 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5005 XmaI C'CCGG_G Cuts at: 1870 2330 1870 Size: 460 4545 XmnI GAAnn'nnTTC Cuts at: 3134 3134 Size: 5005 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2813 276 Size: 53 83 186 936 1279 2468 Fragments arranged by size: 2468 1279 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI