################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaI BseRI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 55 TCCGGA T^CCGGA AcyI 652 GGCGTC G[GA]^CG[CT]C AlwI 975 GGATC GGATC(4/5) Aor51HI 399 AGCGCT AGC^GCT ApaI 470 GGGCCC GGGCC^C AvaII 258 GGACC G^G[AT]CC BalI 428 TGGCCA TGG^CCA BcgI 292 GCAGCCGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 106 CTAG C^TAG BlpI 402 GCTGAGC GC^T[ACGT]AGC Bpu1102I 402 GCTGAGC GC^T[ACGT]AGC BsaHI 652 GGCGTC G[GA]^CG[CT]C BsaWI 55 TCCGGA [AT]^CCGG[AT] BseAI 55 TCCGGA T^CCGGA BsiEI 982 CGGTCG CG[GA][CT]^CG BsmAI 929 GTCTC GTCTC(1/5) BspEI 55 TCCGGA T^CCGGA BsrBI 735 CCGCTC CCGCTC(-3/-3) BstAPI 899 GCAATAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 747 CATCC GGATG(2/0) BstXI 782 CCAACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 975 GGATCT [GA]^GATC[CT] CelII 402 GCTGAGC GC^T[ACGT]AGC DpnII 976 GATC ^GATC DraII 604 GGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 431 CCATGG C^C[GA][CT]GG EarI 463 CTCTTC CTCTTC(1/4) Eco47III 399 AGCGCT AGC^GCT EcoNI 1029 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 604 GGGGCCT [GA]G^G[ACGT]CC[CT] FokI 747 CATCC GGATG(9/13) HaeII 399 AGCGCT [GA]GCGC^[CT] HgaI 653 GCGTC GACGC(5/10) Hin1I 652 GGCGTC G[GA]^CG[CT]C HinfI 298 GAGTC G^A[ACGT]TC Ksp632I 463 CTCTTC CTCTTC(1/4) MaeI 106 CTAG C^TAG MboI 976 GATC ^GATC MflI 975 GGATCT [GA]^GATC[CT] MluNI 428 TGGCCA TGG^CCA MroI 55 TCCGGA T^CCGGA MscI 428 TGGCCA TGG^CCA MslI 747 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 431 CCATGG C^CATGG NdeII 976 GATC ^GATC PleI 298 GAGTC GAGTC(4/5) PshAI 861 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 976 GATC ^GATC ScaI 841 AGTACT AGT^ACT SexAI 824 ACCTGGT A^CC[AT]GGT TaqI 985 TCGA T^CGA TthHB8I 985 TCGA T^CGA XcmI 152 CCATTGGCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 975 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 100 AAATTC [GA]^AATT[CT] 168 AAATTT AlwNI 591 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG 952 CAGGCTCTG AocI 111 CCTGAGG CC^T[ACGT]AGG 933 CCTGAGG ApoI 100 AAATTC [GA]^AATT[CT] 168 AAATTT AvaI 1067 CCCGGG C^[CT]CG[GA]G 1117 CCCGGG BglI 295 GCCGAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 481 GCCACCCTGGC BsmFI 264 GGGAC GGGAC(10/14) 1111 GGGAC BsoBI 1067 CCCGGG C^[CT]CG[GA]G 1117 CCCGGG Bsu36I 111 CCTGAGG CC^T[ACGT]AGG 933 CCTGAGG CfoI 400 GCGC GCG^C 564 GCGC DrdI 229 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 718 GACAGCAGGGTC EaeI 356 CGGCCA [CT]^GGCC[GA] 428 TGGCCA Eco81I 111 CCTGAGG CC^T[ACGT]AGG 933 CCTGAGG EcoT14I 431 CCATGG C^C[AT][AT]GG 647 CCTTGG HhaI 400 GCGC GCG^C 564 GCGC HinP1I 400 GCGC G^CGC 564 GCGC MaeII 371 ACGT A^CGT 862 ACGT PstI 616 CTGCAG CTGCA^G 691 CTGCAG PvuII 527 CAGCTG CAG^CTG 613 CAGCTG SfcI 616 CTGCAG C^T[GA][CT]AG 691 CTGCAG SmaI 1067 CCCGGG CCC^GGG 1117 CCCGGG StyI 431 CCATGG C^C[AT][AT]GG 647 CCTTGG Tsp509I 101 AATT ^AATT 169 AATT XmaCI 1067 CCCGGG C^CCGGG 1117 CCCGGG XmaI 1067 CCCGGG C^CCGGG 1117 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AfaI AluI BanI BanII BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI