(Linear) (Six-base) MAPSORT of: KIEE2255.seq Check: 5700 from: 1 to: 1122 KIEE2255.orf With 182 enzymes: SgfI * March 22, 2012 15:59 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 954 1122 Size: 209 745 168 Fragments arranged by size: 745 209 168 AfeI AGC'GCT Cuts at: 0 401 1122 Size: 401 721 AlwNI CAG_nnn'CTG Cuts at: 0 596 957 1122 Size: 596 361 165 Fragments arranged by size: 596 361 165 ApaI G_GGCC'C Cuts at: 0 474 1122 Size: 474 648 ApoI r'AATT_y Cuts at: 0 100 168 1122 Size: 100 68 954 Fragments arranged by size: 954 100 68 AvaI C'yCGr_G Cuts at: 0 1067 1117 1122 Size: 1067 50 5 Fragments arranged by size: 1067 50 5 BanI G'GyrC_C Cuts at: 0 331 448 519 642 828 964 1122 Size: 331 117 71 123 186 136 158 Fragments arranged by size: 331 186 158 136 123 117 71 BanII G_rGCy'C Cuts at: 0 213 474 947 1122 Size: 213 261 473 175 Fragments arranged by size: 473 261 213 175 BbsI GAAGACnn'nnnn_ Cuts at: 0 231 525 861 1122 Size: 231 294 336 261 Fragments arranged by size: 336 294 261 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 281 315 1122 Size: 281 34 807 Fragments arranged by size: 807 281 34 BglI GCCn_nnn'nGGC Cuts at: 0 301 487 1122 Size: 301 186 635 Fragments arranged by size: 635 301 186 BlpI GC'TnA_GC Cuts at: 0 403 1122 Size: 403 719 Bme1580I G_kGCm'C Cuts at: 0 204 336 474 524 647 833 1122 Size: 204 132 138 50 123 186 289 Fragments arranged by size: 289 204 186 138 132 123 50 BmrI ACTGGGnnnn_n' Cuts at: 0 429 1122 Size: 429 693 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 352 428 1122 Size: 352 76 694 Fragments arranged by size: 694 352 76 BsaHI Gr'CG_yC Cuts at: 0 653 1122 Size: 653 469 BsaWI w'CCGG_w Cuts at: 0 55 1122 Size: 55 1067 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 50 80 263 293 998 1028 1122 Size: 50 30 183 30 705 30 94 Fragments arranged by size: 705 183 94 50 30 30 30 BseYI C'CCAG_C Cuts at: 0 162 586 600 1010 1122 Size: 162 424 14 410 112 Fragments arranged by size: 424 410 162 112 14 BsiEI CG_ry'CG Cuts at: 0 985 1122 Size: 985 137 Bsp1286I G_dGCh'C Cuts at: 0 204 213 336 474 524 647 833 947 Size: 204 9 123 138 50 123 186 114 Cuts at: 947 1122 Size: 175 Fragments arranged by size: 204 186 175 138 123 123 114 50 9 BspEI T'CCGG_A Cuts at: 0 55 1122 Size: 55 1067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 581 680 1026 1122 Size: 581 99 346 96 Fragments arranged by size: 581 346 99 96 BsrBI CCG'CTC Cuts at: 0 737 1122 Size: 737 385 BstAPI GCAn_nnn'nTGC Cuts at: 0 905 1122 Size: 905 217 BstXI CCAn_nnnn'nTGG Cuts at: 0 789 1122 Size: 789 333 BstYI r'GATC_y Cuts at: 0 975 1122 Size: 975 147 Bsu36I CC'TnA_GG Cuts at: 0 112 934 1122 Size: 112 822 188 Fragments arranged by size: 822 188 112 BtgI C'CryG_G Cuts at: 0 431 1122 Size: 431 691 BtsI GCAGTG_nn' Cuts at: 0 69 497 1122 Size: 69 428 625 Fragments arranged by size: 625 428 69 DrdI GACnn_nn'nnGTC Cuts at: 0 235 724 1122 Size: 235 489 398 Fragments arranged by size: 489 398 235 EaeI y'GGCC_r Cuts at: 0 356 428 1122 Size: 356 72 694 Fragments arranged by size: 694 356 72 EarI CTCTTCn'nnn_ Cuts at: 0 469 1122 Size: 469 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 271 1019 1122 Size: 271 748 103 Fragments arranged by size: 748 271 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 954 1122 Size: 209 745 168 Fragments arranged by size: 745 209 168 EcoNI CCTnn'n_nnAGG Cuts at: 0 1033 1122 Size: 1033 89 EcoO109I rG'GnC_Cy Cuts at: 0 605 1122 Size: 605 517 HaeII r_GCGC'y Cuts at: 0 403 1122 Size: 403 719 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 257 289 967 999 1122 Size: 257 32 678 32 123 Fragments arranged by size: 678 257 123 32 32 MscI TGG'CCA Cuts at: 0 430 1122 Size: 430 692 MslI CAynn'nnrTG Cuts at: 0 751 1122 Size: 751 371 MspA1I CmG'CkG Cuts at: 0 529 615 696 1122 Size: 529 86 81 426 Fragments arranged by size: 529 426 86 81 NcoI C'CATG_G Cuts at: 0 431 1122 Size: 431 691 PasI CC'CwG_GG Cuts at: 0 999 1122 Size: 999 123 PshAI GACnn'nnGTC Cuts at: 0 865 1122 Size: 865 257 PspOMI G'GGCC_C Cuts at: 0 470 1122 Size: 470 652 PstI C_TGCA'G Cuts at: 0 620 695 1122 Size: 620 75 427 Fragments arranged by size: 620 427 75 PvuII CAG'CTG Cuts at: 0 529 615 1122 Size: 529 86 507 Fragments arranged by size: 529 507 86 ScaI AGT'ACT Cuts at: 0 843 1122 Size: 843 279 SexAI A'CCwGG_T Cuts at: 0 824 1122 Size: 824 298 SfcI C'TryA_G Cuts at: 0 616 691 1122 Size: 616 75 431 Fragments arranged by size: 616 431 75 SmaI CCC'GGG Cuts at: 0 1069 1119 1122 Size: 1069 50 3 Fragments arranged by size: 1069 50 3 StyI C'CwwG_G Cuts at: 0 431 647 1122 Size: 431 216 475 Fragments arranged by size: 475 431 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 45 411 466 1002 1122 Size: 45 366 55 536 120 Fragments arranged by size: 536 366 120 55 45 TatI w'GTAC_w Cuts at: 0 841 923 1122 Size: 841 82 199 Fragments arranged by size: 841 199 82 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 50 82 263 295 998 1030 1122 Size: 50 32 181 32 703 32 92 Fragments arranged by size: 703 181 92 50 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 159 1122 Size: 159 963 XmaI C'CCGG_G Cuts at: 0 1067 1117 1122 Size: 1067 50 5 Fragments arranged by size: 1067 50 5 Enzymes that do cut: AcuI AfeI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BglI BlpI Bme1580I BmrI BplI BsaHI BsaWI BsaXI BseYI BsiEI Bsp1286I BspEI BspMI BsrBI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I NcoI PasI PshAI PspOMI PstI PvuII ScaI SexAI SfcI SmaI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI