(Circular) (Six-base) MAPSORT of: pFN21AE6550.seq Check: 3780 from: 1 to: 5071 pFN21AE6550 5071 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2881 278 Size: 53 83 186 936 1345 2468 Fragments arranged by size: 2468 1345 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2414 1937 Size: 477 4594 Acc65I G'GTAC_C Cuts at: 2392 2392 Size: 5071 AclI AA'CG_TT Cuts at: 1563 3198 3571 1563 Size: 1635 373 3063 Fragments arranged by size: 3063 1635 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3134 4518 4786 1632 Size: 1502 1384 268 1917 Fragments arranged by size: 1917 1502 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4874 AflIII A'CryG_T Cuts at: 1203 3970 4947 1203 Size: 2767 977 1327 Fragments arranged by size: 2767 1327 977 AgeI A'CCGG_T Cuts at: 3902 3902 Size: 5071 AhdI GACnn_n'nnGTC Cuts at: 3800 3800 Size: 5071 AleI CACnn'nnGTG Cuts at: 1166 2147 1166 Size: 981 4090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2378 1184 Size: 1194 3877 AlwNI CAG_nnn'CTG Cuts at: 2814 4386 2814 Size: 1572 3499 ApaLI G'TGCA_C Cuts at: 3128 4284 3128 Size: 1156 3915 ApoI r'AATT_y Cuts at: 1493 2380 2606 2758 4867 1493 Size: 887 226 152 2109 1697 Fragments arranged by size: 2109 1697 887 226 152 AseI AT'TA_AT Cuts at: 160 1032 3625 160 Size: 872 2593 1606 Fragments arranged by size: 2593 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5071 AvaI C'yCGr_G Cuts at: 1870 1943 2337 2396 1870 Size: 73 394 59 4545 Fragments arranged by size: 4545 394 73 59 BamHI G'GATC_C Cuts at: 2401 2768 2401 Size: 367 4704 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 3847 618 Size: 325 217 627 605 1455 1842 Fragments arranged by size: 1842 1455 627 605 325 217 BanII G_rGCy'C Cuts at: 1391 2141 2390 1391 Size: 750 249 4072 Fragments arranged by size: 4072 750 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3262 3296 1903 Size: 34 50 34 1241 34 3678 Fragments arranged by size: 3678 1241 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2963 4173 2963 Size: 1210 3861 BclI T'GATC_A Cuts at: 1548 1548 Size: 5071 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3682 136 Size: 107 122 71 631 605 2010 1525 Fragments arranged by size: 2010 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5066 5066 Size: 5071 BlpI GC'TnA_GC Cuts at: 2488 2488 Size: 5071 Bme1580I G_kGCm'C Cuts at: 1790 2261 3132 4288 1790 Size: 471 871 1156 2573 Fragments arranged by size: 2573 1156 871 471 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5071 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2802 3760 442 Size: 416 53 83 399 24 1385 958 1753 Fragments arranged by size: 1753 1385 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 3731 1239 Size: 959 1533 2579 Fragments arranged by size: 2579 1533 959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3196 4061 4359 3196 Size: 865 298 3908 Fragments arranged by size: 3908 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3734 882 Size: 617 2235 2219 Fragments arranged by size: 2235 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2862 493 Size: 711 1658 2702 Fragments arranged by size: 2702 1658 711 BsaBI GATnn'nnATC Cuts at: 5065 5065 Size: 5071 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 2878 3260 3895 Size: 53 83 186 936 768 577 382 635 Cuts at: 3895 275 Size: 1451 Fragments arranged by size: 1451 936 768 635 577 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3504 3902 4176 4323 4734 3504 Size: 398 274 147 411 3841 Fragments arranged by size: 3841 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4846 Fragments arranged by size: 4846 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 1186 Size: 862 4209 BseYI C'CCAG_C Cuts at: 4274 4770 4274 Size: 496 4575 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 3282 4310 1540 Size: 275 186 520 761 1028 2301 Fragments arranged by size: 2301 1028 761 520 275 186 BsiHKAI G_wGCw'C Cuts at: 3132 3217 4288 3132 Size: 85 1071 3915 Fragments arranged by size: 3915 1071 85 BsmI GAATG_Cn' Cuts at: 2582 2675 2582 Size: 93 4978 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2261 2390 3132 3217 4288 1391 Size: 399 351 120 129 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 351 129 120 85 BspEI T'CCGG_A Cuts at: 4734 4734 Size: 5071 BspHI T'CATG_A Cuts at: 2287 2960 4690 2287 Size: 673 1730 2668 Fragments arranged by size: 2668 1730 673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2426 844 Size: 1582 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2958 1394 Size: 429 1135 3507 Fragments arranged by size: 3507 1135 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2069 3566 3740 65 Size: 1183 821 1497 174 1396 Fragments arranged by size: 1497 1396 1183 821 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2240 3715 3902 1700 Size: 110 430 1475 187 2869 Fragments arranged by size: 2869 1475 430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5071 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5071 BssSI C'ACGA_G Cuts at: 3131 4143 3131 Size: 1012 4059 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5071 BstBI TT'CG_AA Cuts at: 3875 3875 Size: 5071 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5071 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2149 Size: 5071 BstYI r'GATC_y Cuts at: 1972 2401 2768 3153 3170 4622 4708 4720 5066 Size: 429 367 385 17 1452 86 12 346 Cuts at: 5066 1972 Size: 1977 Fragments arranged by size: 1977 1452 429 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2060 2060 Size: 5071 BtgI C'CryG_G Cuts at: 513 1065 2013 2160 513 Size: 552 948 147 3424 Fragments arranged by size: 3424 948 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4332 Fragments arranged by size: 4332 683 56 BtsI GCAGTG_nn' Cuts at: 2094 2117 2213 2587 3381 3401 2094 Size: 23 96 374 794 20 3764 Fragments arranged by size: 3764 794 374 96 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5036 DraI TTT'AAA Cuts at: 2375 2375 Size: 5071 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4078 809 Size: 507 2762 1802 Fragments arranged by size: 2762 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2210 2277 2518 Size: 54 1425 50 275 18 380 67 241 Cuts at: 2518 3407 5036 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 380 275 241 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2518 1537 Size: 275 706 4090 Fragments arranged by size: 4090 706 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3001 1353 Size: 1648 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2227 3658 4032 4178 387 Size: 1064 138 638 1431 374 146 1280 Fragments arranged by size: 1431 1280 1064 638 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 3134 3731 4518 4786 1239 Size: 393 566 936 597 787 268 1524 Fragments arranged by size: 1524 936 787 597 566 393 268 EcoRI G'AATT_C Cuts at: 2380 2758 2380 Size: 378 4693 FspI TGC'GCA Cuts at: 3577 3577 Size: 5071 HaeII r_GCGC'y Cuts at: 1678 4218 1678 Size: 2540 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 3712 3744 3786 Size: 32 135 32 543 32 1565 32 42 Cuts at: 3786 3818 1373 Size: 32 2626 Fragments arranged by size: 2626 1565 543 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2415 2661 669 Size: 988 281 477 246 3079 Fragments arranged by size: 3079 988 477 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5071 HpaI GTT'AAC Cuts at: 2661 2661 Size: 5071 KpnI G_GTAC'C Cuts at: 2396 2396 Size: 5071 MfeI C'AATT_G Cuts at: 2670 2670 Size: 5071 MluI A'CGCG_T Cuts at: 4947 4947 Size: 5071 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4185 4369 1305 Size: 2880 184 2007 Fragments arranged by size: 2880 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2212 2279 10 Size: 54 1425 343 380 67 2802 Fragments arranged by size: 2802 1425 380 343 67 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 3029 3388 3547 518 Size: 648 981 882 359 159 2042 Fragments arranged by size: 2042 981 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2487 3164 4312 4557 1606 Size: 881 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 677 245 NaeI GCC'GGC Cuts at: 2242 2242 Size: 5071 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4519 NdeI CA'TA_TG Cuts at: 387 387 Size: 5071 NgoMIV G'CCGG_C Cuts at: 2240 2240 Size: 5071 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5071 NotI GC'GGCC_GC Cuts at: 2518 2518 Size: 5071 NspI r_CATG'y Cuts at: 1132 1229 2429 3974 1132 Size: 97 1200 1545 2229 Fragments arranged by size: 2229 1545 1200 97 PasI CC'CwG_GG Cuts at: 1348 2254 1348 Size: 906 4165 PciI A'CATG_T Cuts at: 3970 3970 Size: 5071 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5071 PmeI GTTT'AAAC Cuts at: 2375 2375 Size: 5071 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3140 3172 4653 4685 3140 Size: 32 1481 32 3526 Fragments arranged by size: 3526 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5071 PsiI TTA'TAA Cuts at: 2641 2641 Size: 5071 PstI C_TGCA'G Cuts at: 830 2319 2423 830 Size: 1489 104 3478 Fragments arranged by size: 3478 1489 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5071 SalI G'TCGA_C Cuts at: 1936 2413 1936 Size: 477 4594 SbfI CC_TGCA'GG Cuts at: 2423 2423 Size: 5071 ScaI AGT'ACT Cuts at: 3319 5031 3319 Size: 1712 3359 SfcI C'TryA_G Cuts at: 826 1045 2315 2419 3554 4235 4426 826 Size: 219 1270 104 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 681 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5071 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5071 SmaI CCC'GGG Cuts at: 1872 2398 1872 Size: 526 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3175 4076 4338 820 Size: 197 926 1232 901 262 1553 Fragments arranged by size: 1553 1232 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2862 493 Size: 2369 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5071 SphI G_CATG'C Cuts at: 1132 2429 1132 Size: 1297 3774 SspI AAT'ATT Cuts at: 5 52 2995 5 Size: 47 2943 2081 Fragments arranged by size: 2943 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5071 StyI C'CwwG_G Cuts at: 513 1065 2169 2510 513 Size: 552 1104 341 3074 Fragments arranged by size: 3074 1104 552 341 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 3098 3115 3268 3453 3898 325 Size: 1255 557 961 17 153 185 445 1498 Fragments arranged by size: 1498 1255 961 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3317 5029 96 Size: 274 80 33 51 1443 1340 1712 138 Fragments arranged by size: 1712 1443 1340 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 5031 5063 2192 Size: 32 2807 32 2200 Fragments arranged by size: 2807 2200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5071 XbaI T'CTAG_A Cuts at: 2407 2407 Size: 5071 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2167 2011 Size: 156 4915 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5071 XmaI C'CCGG_G Cuts at: 1870 2396 1870 Size: 526 4545 XmnI GAAnn'nnTTC Cuts at: 3200 3200 Size: 5071 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2879 276 Size: 53 83 186 936 1345 2468 Fragments arranged by size: 2468 1345 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI