################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI MaeIII MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI SalI SbfI ScaI SexAI SfcI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 69 GGCGCC G[GA]^CG[CT]C AfaI 1242 GTAC GT^AC AflIII 551 ACGTGT A^C[GA][CT]GT Alw44I 537 GTGCAC G^TGCAC ApaLI 537 GTGCAC G^TGCAC AvaII 262 GGACC G^G[AT]CC BalI 680 TGGCCA TGG^CCA BamHI 297 GGATCC G^GATCC BbeI 69 GGCGCC GGCGC^C BclI 1106 TGATCA T^GATCA BfaI 688 CTAG C^TAG BlpI 204 GCTGAGC GC^T[ACGT]AGC Bpu1102I 204 GCTGAGC GC^T[ACGT]AGC BsaHI 69 GGCGCC G[GA]^CG[CT]C BsmAI 1005 GTCTC GTCTC(1/5) BsrDI 180 CATTGC GCAATG(2/0) BsrFI 761 ACCGGC [GA]^CCGG[CT] BstAPI 308 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1046 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 204 GCTGAGC GC^T[ACGT]AGC Cfr10I 761 ACCGGC [GA]^CCGG[CT] CpoI 261 CGGACCG CG^G[AT]CCG DraI 961 TTTAAA TTT^AAA EcoNI 116 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1211 CCTTGG C^C[AT][AT]GG FauI 247 GCGGG CCCGC(4/6) FbaI 1106 TGATCA T^GATCA Hin1I 69 GGCGCC G[GA]^CG[CT]C HincII 877 GTTGAC GT[CT]^[GA]AC HindII 877 GTTGAC GT[CT]^[GA]AC KasI 69 GGCGCC G^GCGCC KspI 159 CCGCGG CCGC^GG MaeI 688 CTAG C^TAG MaeII 551 ACGT A^CGT MluNI 680 TGGCCA TGG^CCA MscI 680 TGGCCA TGG^CCA NarI 69 GGCGCC GG^CGCC PaeR7I 142 CTCGAG C^TCGAG RsaI 1242 GTAC GT^AC RsrII 261 CGGACCG CG^G[AT]CCG SacI 775 GAGCTC GAGCT^C SacII 159 CCGCGG CCGC^GG SapI 625 GAAGAGC GCTCTTC(1/4) SfiI 231 GGCCGGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 69 GGCGCC GGC^GCC SmaI 102 CCCGGG CCC^GGG SmlI 142 CTCGAG C^T[CT][GA]AG StyI 1211 CCTTGG C^C[AT][AT]GG Tsp509I 696 AATT ^AATT XhoI 142 CTCGAG C^TCGAG XmaCI 102 CCCGGG C^CCGGG XmaI 102 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 160 CGCG CG^CG 255 CGCG AocI 120 CCTGAGG CC^T[ACGT]AGG 583 CCTGAGG Aor51HI 170 AGCGCT AGC^GCT 889 AGCGCT ApaI 99 GGGCCC GGGCC^C 112 GGGCCC AvaI 102 CCCGGG C^[CT]CG[GA]G 142 CTCGAG BanI 69 GGCGCC G^G[CT][GA]CC 288 GGTGCC BbvCI 487 CCTCAGC CCTCAGC(-5/-2) 634 GCTGAGG BglI 15 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 232 GCCGGAGGGGC BseRI 868 CTCCTC GAGGAG(10/8) 574 GAGGAG BsoBI 102 CCCGGG C^[CT]CG[GA]G 142 CTCGAG BsrI 1128 ACTGG ACTGG(1/-1) 1009 CCAGT BstUI 160 CGCG CG^CG 255 CGCG Bsu36I 120 CCTGAGG CC^T[ACGT]AGG 583 CCTGAGG DsaI 159 CCGCGG C^C[GA][CT]GG 277 CCACGG EaeI 162 CGGCCA [CT]^GGCC[GA] 680 TGGCCA EarI 447 GAAGAG CTCTTC(1/4) 625 GAAGAG Eco47III 170 AGCGCT AGC^GCT 889 AGCGCT Eco81I 120 CCTGAGG CC^T[ACGT]AGG 583 CCTGAGG HphI 1131 GGTGA GGTGA(8/7) 1064 TCACC Ksp632I 447 GAAGAG CTCTTC(1/4) 625 GAAGAG MvnI 160 CGCG CG^CG 255 CGCG NlaIII 1053 CATG CATG^ 1110 CATG PvuII 49 CAGCTG CAG^CTG 632 CAGCTG TaqI 143 TCGA T^CGA 827 TCGA TthHB8I 143 TCGA T^CGA 827 TCGA XcmI 185 CCAGCGCCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 350 CCATCTGGTCGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BssKI BssSI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI TspRI XhoII