################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoRI EcoRV FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MflI MluI MluNI MroI MscI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 43 GGCGCC G[GA]^CG[CT]C AfaI 116 GTAC GT^AC AhdI 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 413 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 355 CCTGAGG CC^T[ACGT]AGG AspEI 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 84 CCCGGG C^[CT]CG[GA]G BanI 43 GGCGCC G^G[CT][GA]CC BanII 694 GAGCCC G[GA]GC[CT]^C BbeI 43 GGCGCC GGCGC^C BbsI 268 GTCTTC GAAGAC(2/6) BpuAI 268 GTCTTC GAAGAC(2/6) BsaBI 602 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 43 GGCGCC G[GA]^CG[CT]C BsaI 536 GAGACC GGTCTC(1/5) BseRI 626 CTCCTC GAGGAG(10/8) BsgI 53 CTGCAC GTGCAG(16/14) BsiEI 18 CGACCG CG[GA][CT]^CG BsmFI 164 GGGAC GGGAC(10/14) BsoBI 84 CCCGGG C^[CT]CG[GA]G BspHI 402 TCATGA T^CATGA BsrI 428 CCAGT ACTGG(1/-1) BstEII 423 GGTTACC G^GT[ACGT]ACC BstPI 423 GGTTACC G^GT[ACGT]ACC Bsu36I 355 CCTGAGG CC^T[ACGT]AGG DraI 495 TTTAAA TTT^AAA DsaI 39 CCACGG C^C[GA][CT]GG EaeI 75 CGGCCA [CT]^GGCC[GA] Eam1105I 19 GACCGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 355 CCTGAGG CC^T[ACGT]AGG EcoNI 351 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 423 GGTTACC G^GT[ACGT]ACC EcoT14I 47 CCAAGG C^C[AT][AT]GG EcoT22I 725 ATGCAT ATGCA^T Hin1I 43 GGCGCC G[GA]^CG[CT]C HphI 550 TCACC GGTGA(8/7) KasI 43 GGCGCC G^GCGCC MamI 602 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 291 CAATTG C^AATTG MslI 718 CATCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 243 CCGCTG C[AC]G^C[GT]G MunI 291 CAATTG C^AATTG NarI 43 GGCGCC GG^CGCC NsiI 725 ATGCAT ATGCA^T PpuMI 280 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 402 TCATGA T^CATGA RsaI 116 GTAC GT^AC SfaNI 490 GATGC GCATC(5/9) SfoI 43 GGCGCC GGC^GCC SmaI 84 CCCGGG CCC^GGG SspI 664 AATATT AAT^ATT StyI 47 CCAAGG C^C[AT][AT]GG Tsp45I 674 GTGAC ^GT[GC]AC XmaCI 84 CCCGGG C^CCGGG XmaI 84 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AciI 243 CCGC CCGC(-3/-1) 74 GCGG AlwI 406 GATCC GGATC(4/5) 456 GATCC BbvI 186 GCAGC GCAGC(8/12) 52 GCTGC BsmAI 523 GAGAC GTCTC(1/5) 536 GAGAC DraII 280 GGGTCCT [GA]G^G[ACGT]CC[CT] 558 AGGGCCT EcoO109I 280 GGGTCCT [GA]G^G[ACGT]CC[CT] 558 AGGGCCT HaeII 35 AGCGCC [GA]GCGC^[CT] 43 GGCGCC MboII 780 GAAGA GAAGA(8/7) 269 TCTTC NlaIV 43 GGCGCC GG[ACGT]^[ACGT]CC 280 GGGTCC PleI 148 GACTC GAGTC(4/5) 202 GACTC TaqI 488 TCGA T^CGA 588 TCGA TseI 52 GCTGC G^C[AT]GC 186 GCAGC TthHB8I 488 TCGA T^CGA 588 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AluI AspHI AvaII BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII Sau3AI Sau96I ScrFI TfiI Tru9I Tsp509I TspRI