(Circular) (Six-base) MAPSORT of: pFN21AE5328.seq Check: 1015 from: 1 to: 5521 pFN21AE5328 5521 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3331 278 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2864 1937 Size: 927 4594 Acc65I G'GTAC_C Cuts at: 2842 2842 Size: 5521 AclI AA'CG_TT Cuts at: 1563 3648 4021 1563 Size: 2085 373 3063 Fragments arranged by size: 3063 2085 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 3584 4968 5236 1632 Size: 645 1307 1384 268 1917 Fragments arranged by size: 1917 1384 1307 645 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5324 AflIII A'CryG_T Cuts at: 1203 4420 5397 1203 Size: 3217 977 1327 Fragments arranged by size: 3217 1327 977 AgeI A'CCGG_T Cuts at: 4352 4352 Size: 5521 AhdI GACnn_n'nnGTC Cuts at: 2029 4250 2029 Size: 2221 3300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5521 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2388 Size: 32 5489 AlwNI CAG_nnn'CTG Cuts at: 2423 3264 4836 2423 Size: 841 1572 3108 Fragments arranged by size: 3108 1572 841 ApaLI G'TGCA_C Cuts at: 3578 4734 3578 Size: 1156 4365 ApoI r'AATT_y Cuts at: 1493 2830 3056 3208 5317 1493 Size: 1337 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 AseI AT'TA_AT Cuts at: 160 1032 4075 160 Size: 872 3043 1606 Fragments arranged by size: 3043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2846 1870 Size: 73 146 757 4545 Fragments arranged by size: 4545 757 146 73 BamHI G'GATC_C Cuts at: 2851 3218 2851 Size: 367 5154 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2842 4297 618 Size: 325 217 627 261 794 1455 1842 Fragments arranged by size: 1842 1455 794 627 325 261 217 BanII G_rGCy'C Cuts at: 1391 2703 2840 1391 Size: 1312 137 4072 Fragments arranged by size: 4072 1312 137 BbeI G_GCGC'C Cuts at: 2052 2052 Size: 5521 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2266 928 Size: 971 367 4183 Fragments arranged by size: 4183 971 367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3712 3746 1903 Size: 34 1775 34 3678 Fragments arranged by size: 3678 1775 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3413 4623 3413 Size: 1210 4311 BclI T'GATC_A Cuts at: 1548 1548 Size: 5521 BfrBI ATG'CAT Cuts at: 2732 2732 Size: 5521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4132 136 Size: 107 122 71 631 605 2460 1525 Fragments arranged by size: 2460 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5516 5516 Size: 5521 BlpI GC'TnA_GC Cuts at: 2938 2938 Size: 5521 Bme1580I G_kGCm'C Cuts at: 1790 3582 4738 1790 Size: 1792 1156 2573 Fragments arranged by size: 2573 1792 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3252 4210 442 Size: 416 53 83 399 24 1835 958 1753 Fragments arranged by size: 1835 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4181 1239 Size: 2942 2579 Bpu10I CC'TnA_GC Cuts at: 2697 2762 2697 Size: 65 5456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4511 4809 3646 Size: 865 298 4358 Fragments arranged by size: 4358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2535 4184 882 Size: 617 1036 1649 2219 Fragments arranged by size: 2219 1649 1036 617 BsaAI yAC'GTr Cuts at: 493 1204 3312 493 Size: 711 2108 2702 Fragments arranged by size: 2702 2108 711 BsaBI GATnn'nnATC Cuts at: 2611 5515 2611 Size: 2904 2617 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 3328 3710 4345 Size: 53 83 186 936 516 1279 382 635 Cuts at: 4345 275 Size: 1451 Fragments arranged by size: 1451 1279 936 635 516 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3954 4352 4626 4773 5184 3954 Size: 398 274 147 411 4291 Fragments arranged by size: 4291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5296 Fragments arranged by size: 5296 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2622 1186 Size: 1436 4085 BseYI C'CCAG_C Cuts at: 2132 2683 4724 5220 2132 Size: 551 2041 496 2433 Fragments arranged by size: 2433 2041 551 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2043 1163 Size: 573 307 4641 Fragments arranged by size: 4641 573 307 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2026 2971 3732 4760 1540 Size: 275 186 25 945 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 275 186 25 BsiHKAI G_wGCw'C Cuts at: 2103 2306 2373 3582 3667 4738 2103 Size: 203 67 1209 85 1071 2886 Fragments arranged by size: 2886 1209 1071 203 85 67 BsmI GAATG_Cn' Cuts at: 3032 3125 3032 Size: 93 5428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2306 2373 2703 2840 3582 3667 Size: 399 313 203 67 330 137 742 85 Cuts at: 3667 4738 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 330 313 203 137 85 67 BspEI T'CCGG_A Cuts at: 5184 5184 Size: 5521 BspHI T'CATG_A Cuts at: 2407 3410 5140 2407 Size: 1003 1730 2788 Fragments arranged by size: 2788 1730 1003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2876 844 Size: 2032 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3408 1394 Size: 429 1585 3507 Fragments arranged by size: 3507 1585 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4016 4190 65 Size: 1183 2768 174 1396 Fragments arranged by size: 2768 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4165 4352 1700 Size: 110 2355 187 2869 Fragments arranged by size: 2869 2355 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5521 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5521 BssSI C'ACGA_G Cuts at: 3581 4593 3581 Size: 1012 4509 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5521 BstBI TT'CG_AA Cuts at: 4325 4325 Size: 5521 BstEII G'GTnAC_C Cuts at: 1184 2428 1184 Size: 1244 4277 BstYI r'GATC_y Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516 Size: 879 367 385 17 1452 86 12 346 Cuts at: 5516 1972 Size: 1977 Fragments arranged by size: 1977 1452 879 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2361 2361 Size: 5521 BtgI C'CryG_G Cuts at: 513 1065 2004 2044 513 Size: 552 939 40 3990 Fragments arranged by size: 3990 939 552 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2759 487 Size: 683 56 1533 3249 Fragments arranged by size: 3249 1533 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3831 3851 3037 Size: 794 20 4707 Fragments arranged by size: 4707 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5486 DraI TTT'AAA Cuts at: 2502 2825 2502 Size: 323 5198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4528 809 Size: 507 3212 1802 Fragments arranged by size: 3212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2968 3857 Size: 54 1425 50 275 18 250 888 889 Cuts at: 3857 5486 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 888 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2968 1537 Size: 275 1156 4090 Fragments arranged by size: 4090 1156 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3451 1353 Size: 2098 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4108 4482 4628 387 Size: 1064 138 2519 374 146 1280 Fragments arranged by size: 2519 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 3584 4181 4968 5236 1239 Size: 393 645 1307 597 787 268 1524 Fragments arranged by size: 1524 1307 787 645 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 5521 EcoO109I rG'GnC_Cy Cuts at: 2286 2564 2286 Size: 278 5243 EcoRI G'AATT_C Cuts at: 2830 3208 2830 Size: 378 5143 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2290 2669 2290 Size: 379 5142 FspI TGC'GCA Cuts at: 4027 4027 Size: 5521 HaeII r_GCGC'y Cuts at: 1678 2044 2052 4668 1678 Size: 366 8 2616 2531 Fragments arranged by size: 2616 2531 366 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2168 2200 4162 Size: 32 135 32 443 32 121 32 1962 Cuts at: 4162 4194 4236 4268 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1962 443 135 121 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2865 3111 669 Size: 988 281 927 246 3079 Fragments arranged by size: 3079 988 927 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5521 HpaI GTT'AAC Cuts at: 3111 3111 Size: 5521 KasI G'GCGC_C Cuts at: 2048 2048 Size: 5521 KpnI G_GTAC'C Cuts at: 2846 2846 Size: 5521 MfeI C'AATT_G Cuts at: 2296 3120 2296 Size: 824 4697 MluI A'CGCG_T Cuts at: 5397 5397 Size: 5521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4635 4819 1305 Size: 3330 184 2007 Fragments arranged by size: 3330 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3699 Fragments arranged by size: 3699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2727 3479 3838 3997 518 Size: 648 1561 752 359 159 2042 Fragments arranged by size: 2042 1561 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2250 2937 3614 4762 5007 1606 Size: 644 687 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 644 245 NarI GG'CG_CC Cuts at: 2049 2049 Size: 5521 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4030 Fragments arranged by size: 4030 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5521 NotI GC'GGCC_GC Cuts at: 2968 2968 Size: 5521 NsiI A_TGCA'T Cuts at: 2734 2734 Size: 5521 NspI r_CATG'y Cuts at: 1132 1229 2879 4424 1132 Size: 97 1650 1545 2229 Fragments arranged by size: 2229 1650 1545 97 PasI CC'CwG_GG Cuts at: 1348 2165 2561 1348 Size: 817 396 4308 Fragments arranged by size: 4308 817 396 PciI A'CATG_T Cuts at: 4420 4420 Size: 5521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5521 PfoI T'CCnGG_A Cuts at: 2349 2349 Size: 5521 PmeI GTTT'AAAC Cuts at: 2825 2825 Size: 5521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3622 5103 5135 3590 Size: 32 1481 32 3976 Fragments arranged by size: 3976 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2286 2286 Size: 5521 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5521 PsiI TTA'TAA Cuts at: 3091 3091 Size: 5521 PstI C_TGCA'G Cuts at: 830 2873 830 Size: 2043 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5521 SalI G'TCGA_C Cuts at: 1936 2863 1936 Size: 927 4594 SbfI CC_TGCA'GG Cuts at: 2873 2873 Size: 5521 ScaI AGT'ACT Cuts at: 3769 5481 3769 Size: 1712 3809 SfcI C'TryA_G Cuts at: 826 1045 2869 4004 4685 4876 826 Size: 219 1824 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2050 2050 Size: 5521 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5521 SmaI CCC'GGG Cuts at: 1872 2091 2848 1872 Size: 219 757 4545 Fragments arranged by size: 4545 757 219 SmlI C'TyrA_G Cuts at: 820 1017 1943 3625 4526 4788 820 Size: 197 926 1682 901 262 1553 Fragments arranged by size: 1682 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3312 493 Size: 2819 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5521 SphI G_CATG'C Cuts at: 1132 2879 1132 Size: 1747 3774 SspI AAT'ATT Cuts at: 5 52 2671 3445 5 Size: 47 2619 774 2081 Fragments arranged by size: 2619 2081 774 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2960 513 Size: 552 939 48 908 3074 Fragments arranged by size: 3074 939 908 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2635 3548 3565 3718 3903 4348 325 Size: 1255 1055 913 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1055 913 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3767 5479 96 Size: 274 80 33 51 1443 1790 1712 138 Fragments arranged by size: 1790 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2296 2328 2384 2416 5481 5513 2296 Size: 32 56 32 3065 32 2304 Fragments arranged by size: 3065 2304 56 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5521 XbaI T'CTAG_A Cuts at: 2857 2857 Size: 5521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5521 XmaI C'CCGG_G Cuts at: 1870 2089 2846 1870 Size: 219 757 4545 Fragments arranged by size: 4545 757 219 XmnI GAAnn'nnTTC Cuts at: 3650 3650 Size: 5521 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3329 276 Size: 53 83 186 936 1795 2468 Fragments arranged by size: 2468 1795 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI