(Circular) (Six-base) MAPSORT of: pFN21AE6656.seq Check: 2062 from: 1 to: 5482 pFN21AE6656 5482 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2008 3545 4929 5197 1632 Size: 376 1537 1384 268 1917 Fragments arranged by size: 1917 1537 1384 376 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 3225 4797 3225 Size: 1572 3910 ApaI G_GGCC'C Cuts at: 2365 2365 Size: 5482 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2404 2791 3017 3169 5278 1493 Size: 911 387 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 387 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2598 2807 1870 Size: 73 100 555 209 4545 Fragments arranged by size: 4545 555 209 100 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 4258 618 Size: 325 217 627 1016 1455 1842 Fragments arranged by size: 1842 1455 1016 627 325 217 BanII G_rGCy'C Cuts at: 1391 2365 2801 1391 Size: 974 436 4072 Fragments arranged by size: 4072 974 436 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 928 Size: 971 546 3965 Fragments arranged by size: 3965 971 546 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4093 136 Size: 107 122 71 631 605 2421 1525 Fragments arranged by size: 2421 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2899 2899 Size: 5482 Bme1580I G_kGCm'C Cuts at: 1790 2365 3543 4699 1790 Size: 575 1178 1156 2573 Fragments arranged by size: 2573 1178 1156 575 BmgBI CAC'GTC Cuts at: 1318 2635 1318 Size: 1317 4165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2062 2523 3213 Size: 416 53 83 399 24 645 461 690 Cuts at: 3213 4171 442 Size: 958 1753 Fragments arranged by size: 1753 958 690 645 461 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2505 2523 4142 1239 Size: 929 337 18 1619 2579 Fragments arranged by size: 2579 1619 929 337 18 Bpu10I CC'TnA_GC Cuts at: 2159 2159 Size: 5482 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 2759 3607 4472 4770 2228 Size: 531 848 865 298 2940 Fragments arranged by size: 2940 865 848 531 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3273 493 Size: 711 2069 2702 Fragments arranged by size: 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 3289 3671 4306 Size: 53 83 186 936 477 1279 382 635 Cuts at: 4306 275 Size: 1451 Fragments arranged by size: 1451 1279 936 635 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3915 4313 4587 4734 5145 3915 Size: 398 274 147 411 4252 Fragments arranged by size: 4252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2435 2465 2478 Size: 30 165 30 636 30 366 30 13 Cuts at: 2478 2508 1178 Size: 30 4152 Fragments arranged by size: 4152 636 366 165 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2502 2505 2559 1186 Size: 1316 3 54 4109 Fragments arranged by size: 4109 1316 54 3 BseYI C'CCAG_C Cuts at: 2364 4685 5181 2364 Size: 2321 496 2665 Fragments arranged by size: 2665 2321 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4909 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2032 3543 3628 4699 2032 Size: 1511 85 1071 2815 Fragments arranged by size: 2815 1511 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2365 2801 3543 3628 4699 1391 Size: 399 242 333 436 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 436 399 333 242 85 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 2400 3371 5101 2400 Size: 971 1730 2781 Fragments arranged by size: 2781 1730 971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2837 844 Size: 1993 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2050 3369 1394 Size: 429 227 1319 3507 Fragments arranged by size: 3507 1319 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2466 4126 4313 1700 Size: 110 656 1660 187 2869 Fragments arranged by size: 2869 1660 656 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 2723 3542 4554 2723 Size: 819 1012 3651 Fragments arranged by size: 3651 1012 819 BstAPI GCAn_nnn'nTGC Cuts at: 794 2587 794 Size: 1793 3689 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477 Size: 840 367 385 17 1452 86 12 346 Cuts at: 5477 1972 Size: 1977 Fragments arranged by size: 1977 1452 840 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2998 3792 3812 2998 Size: 794 20 4668 Fragments arranged by size: 4668 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 4489 809 Size: 507 699 2474 1802 Fragments arranged by size: 2474 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2929 3818 Size: 54 1425 50 275 18 259 840 889 Cuts at: 3818 5447 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 840 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 2663 3412 1353 Size: 735 575 749 3423 Fragments arranged by size: 3423 749 735 575 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4069 4443 4589 387 Size: 1064 138 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2168 2505 2523 3545 4142 4929 Size: 393 376 160 337 18 1022 597 787 Cuts at: 4929 5197 1239 Size: 268 1524 Fragments arranged by size: 1524 1022 787 597 393 376 337 268 160 18 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 2692 4629 1678 Size: 1014 1937 2531 Fragments arranged by size: 2531 1937 1014 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2244 2276 2435 2467 2478 Size: 32 135 32 672 32 159 32 11 Cuts at: 2478 2510 4123 4155 4197 4229 1373 Size: 32 1613 32 42 32 2626 Fragments arranged by size: 2626 1613 672 159 135 42 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 2174 748 Size: 1426 4056 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4596 4780 1305 Size: 3291 184 2007 Fragments arranged by size: 3291 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 10 Size: 54 1425 343 259 3401 Fragments arranged by size: 3401 1425 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2898 3575 4723 4968 1606 Size: 1292 677 1148 245 2120 Fragments arranged by size: 2120 1292 1148 677 245 NaeI GCC'GGC Cuts at: 2468 2468 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NgoMIV G'CCGG_C Cuts at: 2466 2466 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2840 4385 1132 Size: 97 1611 1545 2229 Fragments arranged by size: 2229 1611 1545 97 PasI CC'CwG_GG Cuts at: 1348 2699 1348 Size: 1351 4131 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PfoI T'CCnGG_A Cuts at: 2539 2539 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PspOMI G'GGCC_C Cuts at: 2361 2361 Size: 5482 PspXI vC'TCGA_Gb Cuts at: 2598 2598 Size: 5482 PstI C_TGCA'G Cuts at: 830 2103 2834 830 Size: 1273 731 3478 Fragments arranged by size: 3478 1273 731 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2099 2830 3965 4646 4837 826 Size: 219 1054 731 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 731 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2045 2809 1872 Size: 173 764 4545 Fragments arranged by size: 4545 764 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 2598 2774 3586 4487 4749 Size: 197 926 264 391 176 812 901 262 Cuts at: 4749 820 Size: 1553 Fragments arranged by size: 1553 926 901 812 391 264 262 197 176 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 3406 5 Size: 47 3354 2081 Fragments arranged by size: 3354 2081 47 StuI AGG'CCT Cuts at: 1517 2682 1517 Size: 1165 4317 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 2921 513 Size: 552 939 160 757 3074 Fragments arranged by size: 3074 939 757 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3509 3526 3679 3864 4309 325 Size: 1255 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2289 3728 5440 Size: 274 80 33 51 1443 312 1439 1712 Cuts at: 5440 96 Size: 138 Fragments arranged by size: 1712 1443 1439 312 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 5442 5474 2039 Size: 32 3371 32 2047 Fragments arranged by size: 3371 2047 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2306 2636 1316 Size: 990 330 4162 Fragments arranged by size: 4162 990 330 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2162 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 2598 1943 Size: 655 4827 XmaI C'CCGG_G Cuts at: 1870 2043 2807 1870 Size: 173 764 4545 Fragments arranged by size: 4545 764 173 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI