Restriction map for
pFN21AE6505
(Circular) (Six-base) MAPSORT of: pFN21AE6505.seq Check: 397 from: 1 to: 5305
pFN21AE6505 5305 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3115 278
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2648 1937
Size: 711 4594
Acc65I G'GTAC_C
Cuts at: 2626 2626
Size: 5305
AclI AA'CG_TT
Cuts at: 1563 3432 3805 1563
Size: 1869 373 3063
Fragments arranged by size:
3063 1869 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3368 4752 5020 1632
Size: 1736 1384 268 1917
Fragments arranged by size:
1917 1736 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5108
AflIII A'CryG_T
Cuts at: 1203 4204 5181 1203
Size: 3001 977 1327
Fragments arranged by size:
3001 1327 977
AgeI A'CCGG_T
Cuts at: 4136 4136
Size: 5305
AhdI GACnn_n'nnGTC
Cuts at: 4034 4034
Size: 5305
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5305
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5305
AlwNI CAG_nnn'CTG
Cuts at: 2266 2528 3048 4620 2266
Size: 262 520 1572 2951
Fragments arranged by size:
2951 1572 520 262
ApaLI G'TGCA_C
Cuts at: 3362 4518 3362
Size: 1156 4149
ApoI r'AATT_y
Cuts at: 1493 2614 2840 2992 5101 1493
Size: 1121 226 152 2109 1697
Fragments arranged by size:
2109 1697 1121 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3859 160
Size: 872 2827 1606
Fragments arranged by size:
2827 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
AvaI C'yCGr_G
Cuts at: 1870 1943 2478 2630 1870
Size: 73 535 152 4545
Fragments arranged by size:
4545 535 152 73
BamHI G'GATC_C
Cuts at: 2105 2635 3002 2105
Size: 530 367 4408
Fragments arranged by size:
4408 530 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2286 2626 4081 618
Size: 325 217 627 499 340 1455 1842
Fragments arranged by size:
1842 1455 627 499 340 325 217
BanII G_rGCy'C
Cuts at: 1391 2580 2624 1391
Size: 1189 44 4072
Fragments arranged by size:
4072 1189 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4334
BbvCI CC'TCA_GC
Cuts at: 2040 2545 2040
Size: 505 4800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3496 3530 1903
Size: 34 50 34 1475 34 3678
Fragments arranged by size:
3678 1475 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3197 4407 3197
Size: 1210 4095
BclI T'GATC_A
Cuts at: 1548 2020 1548
Size: 472 4833
BfrBI ATG'CAT
Cuts at: 2016 2016
Size: 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3916 136
Size: 107 122 71 631 605 2244 1525
Fragments arranged by size:
2244 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5300 5300
Size: 5305
BlpI GC'TnA_GC
Cuts at: 2722 2722
Size: 5305
Bme1580I G_kGCm'C
Cuts at: 1790 2291 3366 4522 1790
Size: 501 1075 1156 2573
Fragments arranged by size:
2573 1156 1075 501
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5305
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3036 3994 442
Size: 416 53 83 399 24 1619 958 1753
Fragments arranged by size:
1753 1619 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 3965 1239
Size: 1250 1476 2579
Fragments arranged by size:
2579 1476 1250
Bpu10I CC'TnA_GC
Cuts at: 2040 2457 2545 2040
Size: 417 88 4800
Fragments arranged by size:
4800 417 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3430 4295 4593 3430
Size: 865 298 4142
Fragments arranged by size:
4142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3968 882
Size: 617 2469 2219
Fragments arranged by size:
2469 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3096 493
Size: 711 1892 2702
Fragments arranged by size:
2702 1892 711
BsaBI GATnn'nnATC
Cuts at: 5299 5299
Size: 5305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3112 3494 4129 275
Size: 53 83 186 936 1579 382 635 1451
Fragments arranged by size:
1579 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3738 4136 4410 4557 4968 3738
Size: 398 274 147 411 4075
Fragments arranged by size:
4075 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5080
Fragments arranged by size:
5080 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5305
BseYI C'CCAG_C
Cuts at: 2198 2528 4508 5004 2198
Size: 330 1980 496 2499
Fragments arranged by size:
2499 1980 496 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4732
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2755 3516 4544 1540
Size: 275 186 754 761 1028 2301
Fragments arranged by size:
2301 1028 761 754 275 186
BsiHKAI G_wGCw'C
Cuts at: 3366 3451 4522 3366
Size: 85 1071 4149
Fragments arranged by size:
4149 1071 85
BsmI GAATG_Cn'
Cuts at: 2018 2598 2816 2909 2018
Size: 580 218 93 4414
Fragments arranged by size:
4414 580 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2291 2580 2624 3366 3451 4522 1391
Size: 399 501 289 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 501 399 289 85 44
BspEI T'CCGG_A
Cuts at: 4968 4968
Size: 5305
BspHI T'CATG_A
Cuts at: 3194 4924 3194
Size: 1730 3575
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2660 844
Size: 1430 386 3489
Fragments arranged by size:
3489 1430 386
BsrBI CCG'CTC
Cuts at: 1394 1823 3192 1394
Size: 429 1369 3507
Fragments arranged by size:
3507 1369 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3800 3974 65
Size: 1183 2552 174 1396
Fragments arranged by size:
2552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3949 4136 1700
Size: 110 2139 187 2869
Fragments arranged by size:
2869 2139 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5305
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5305
BssSI C'ACGA_G
Cuts at: 3365 4377 3365
Size: 1012 4293
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5305
BstBI TT'CG_AA
Cuts at: 4109 4109
Size: 5305
BstEII G'GTnAC_C
Cuts at: 1184 2090 1184
Size: 906 4399
BstYI r'GATC_y
Cuts at: 1972 2105 2151 2635 3002 3387 3404 4856 4942
Size: 133 46 484 367 385 17 1452 86
Cuts at: 4942 4954 5300 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 484 385 367 346 133 86
46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2241 2448 513
Size: 552 1176 207 3370
Fragments arranged by size:
3370 1176 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4566
Fragments arranged by size:
4566 683 56
BtsI GCAGTG_nn'
Cuts at: 2821 3615 3635 2821
Size: 794 20 4491
Fragments arranged by size:
4491 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5270
DraI TTT'AAA
Cuts at: 2609 2609
Size: 5305
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4312 809
Size: 507 2996 1802
Fragments arranged by size:
2996 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2752 3641
Size: 54 1425 50 275 18 408 514 889
Cuts at: 3641 5270 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 514 408 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2752 1537
Size: 275 940 4090
Fragments arranged by size:
4090 940 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2519 3235 1353
Size: 1166 716 3423
Fragments arranged by size:
3423 1166 716
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3892 4266 4412 387
Size: 1064 138 2303 374 146 1280
Fragments arranged by size:
2303 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 3368 3965 4752 5020 1239
Size: 393 857 879 597 787 268 1524
Fragments arranged by size:
1524 879 857 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2263 2263
Size: 5305
EcoRI G'AATT_C
Cuts at: 2614 2992 2614
Size: 378 4927
FspI TGC'GCA
Cuts at: 3811 3811
Size: 5305
HaeII r_GCGC'y
Cuts at: 1678 4452 1678
Size: 2774 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3946 3978 4020 4052 1373
Size: 32 135 32 2374 32 42 32 2626
Fragments arranged by size:
2626 2374 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2649 2895 669
Size: 988 281 711 246 3079
Fragments arranged by size:
3079 988 711 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5305
HpaI GTT'AAC
Cuts at: 2895 2895
Size: 5305
KpnI G_GTAC'C
Cuts at: 2630 2630
Size: 5305
MfeI C'AATT_G
Cuts at: 2904 2904
Size: 5305
MluI A'CGCG_T
Cuts at: 5181 5181
Size: 5305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4419 4603 1305
Size: 3114 184 2007
Fragments arranged by size:
3114 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 10
Size: 54 1425 343 408 3075
Fragments arranged by size:
3075 1425 408 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2406 3263 3622 3781 518
Size: 648 1240 857 359 159 2042
Fragments arranged by size:
2042 1240 857 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2255 2721 3398 4546 4791 1606
Size: 592 57 466 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 592 466 245 57
NcoI C'CATG_G
Cuts at: 513 1065 2241 513
Size: 552 1176 3577
Fragments arranged by size:
3577 1176 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5305
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5305
NotI GC'GGCC_GC
Cuts at: 2752 2752
Size: 5305
NsiI A_TGCA'T
Cuts at: 2018 2018
Size: 5305
NspI r_CATG'y
Cuts at: 1132 1229 2663 4208 1132
Size: 97 1434 1545 2229
Fragments arranged by size:
2229 1545 1434 97
PasI CC'CwG_GG
Cuts at: 1348 2135 1348
Size: 787 4518
PciI A'CATG_T
Cuts at: 4204 4204
Size: 5305
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5305
PmeI GTTT'AAAC
Cuts at: 2609 2609
Size: 5305
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4887 4919 3374
Size: 32 1481 32 3760
Fragments arranged by size:
3760 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 2263
Size: 5305
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5305
PsiI TTA'TAA
Cuts at: 2875 2875
Size: 5305
PstI C_TGCA'G
Cuts at: 830 2271 2657 830
Size: 1441 386 3478
Fragments arranged by size:
3478 1441 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5305
PvuII CAG'CTG
Cuts at: 2198 2255 2198
Size: 57 5248
SalI G'TCGA_C
Cuts at: 1936 2647 1936
Size: 711 4594
SbfI CC_TGCA'GG
Cuts at: 2657 2657
Size: 5305
ScaI AGT'ACT
Cuts at: 3553 5265 3553
Size: 1712 3593
SfcI C'TryA_G
Cuts at: 826 1045 2058 2267 2653 3788 4469 4660 826
Size: 219 1013 209 386 1135 681 191 1471
Fragments arranged by size:
1471 1135 1013 681 386 219 209 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5305
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5305
SmaI CCC'GGG
Cuts at: 1872 2632 1872
Size: 760 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3409 4310 4572 820
Size: 197 926 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3096 493
Size: 2603 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5305
SphI G_CATG'C
Cuts at: 1132 2663 1132
Size: 1531 3774
SspI AAT'ATT
Cuts at: 5 52 3229 5
Size: 47 3177 2081
Fragments arranged by size:
3177 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5305
StyI C'CwwG_G
Cuts at: 513 1065 2241 2744 513
Size: 552 1176 503 3074
Fragments arranged by size:
3074 1176 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2109 3332 3349 3502 3687 4132 325
Size: 1255 529 1223 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1223 529 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3551 5263 96
Size: 274 80 33 51 1443 1574 1712 138
Fragments arranged by size:
1712 1574 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5265 5297 5265
Size: 32 5273
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5305
XbaI T'CTAG_A
Cuts at: 2641 2641
Size: 5305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5305
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5305
XmaI C'CCGG_G
Cuts at: 1870 2630 1870
Size: 760 4545
XmnI GAAnn'nnTTC
Cuts at: 2376 3434 2376
Size: 1058 4247
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3113 276
Size: 53 83 186 936 1579 2468
Fragments arranged by size:
2468 1579 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI