################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeI MamI MluI MluNI MroI MscI MseI MslI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AlwI 18 GATCC GGATC(4/5) AlwNI 368 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 637 GTGCTC G[AT]GC[AT]^C AvaII 237 GGTCC G^G[AT]CC BanI 376 GGCACC G^G[CT][GA]CC BbvCI 128 CCTCAGC CCTCAGC(-5/-2) BclI 315 TGATCA T^GATCA BsaI 490 GAGACC GGTCTC(1/5) BsgI 165 CTGCAC GTGCAG(16/14) BsiHKAI 637 GTGCTC G[AT]GC[AT]^C BsmI 417 GAATGC GAATGC(1/-1) BsrI 439 ACTGG ACTGG(1/-1) BstAPI 304 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 17 AGATCC [GA]^GATC[CT] Cfr13I 237 GGTCC G^G[ACGT]CC FbaI 315 TGATCA T^GATCA HaeIII 295 GGCC GG^CC HincII 553 GTTGAC GT[CT]^[GA]AC HindII 553 GTTGAC GT[CT]^[GA]AC HphI 747 TCACC GGTGA(8/7) MaeII 356 ACGT A^CGT MfeI 65 CAATTG C^AATTG MflI 17 AGATCC [GA]^GATC[CT] MunI 65 CAATTG C^AATTG NspI 452 GCATGC [GA]CATG^[CT] PflMI 800 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 744 GAGTC GAGTC(4/5) Sau96I 237 GGTCC G^G[ACGT]CC SfcI 751 CTACAG C^T[GA][CT]AG SmlI 427 CTTGAG C^T[CT][GA]AG SphI 452 GCATGC GCATG^C Van91I 800 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 17 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbvI 164 GCTGC GCAGC(8/12) 756 GCTGC BmyI 637 GTGCTC G[AGT]GC[ACT]^C 809 GGGCAC Bsp1286I 637 GTGCTC G[AGT]GC[ACT]^C 809 GGGCAC BsrDI 690 CATTGC GCAATG(2/0) 332 GCAATG BssKI 9 CCTGG ^CC[ACGT]GG 558 CCAGG BstNI 9 CCTGG CC^[AT]GG 558 CCAGG EarI 22 CTCTTC CTCTTC(1/4) 786 CTCTTC EcoRII 9 CCTGG ^CC[AT]GG 558 CCAGG EcoRV 196 GATATC GAT^ATC 709 GATATC HapII 297 CCGG C^CGG 457 CCGG HpaII 297 CCGG C^CGG 457 CCGG Ksp632I 22 CTCTTC CTCTTC(1/4) 786 CTCTTC MboII 23 TCTTC GAAGA(8/7) 787 TCTTC MspI 297 CCGG C^CGG 457 CCGG MvaI 9 CCTGG CC^[AT]GG 558 CCAGG ScrFI 9 CCTGG CC^[ACGT]GG 558 CCAGG TfiI 155 GAATC G^A[AT]TC 724 GAATC TseI 164 GCTGC G^C[AT]GC 756 GCTGC Tsp509I 36 AATT ^AATT 66 AATT TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^ 533 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AluI BpmI BsaJI BsiYI BslI BsmAI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HinfI ItaI MaeIII MboI MnlI MspA1I MwoI NdeII NlaIII NlaIV PvuII Sau3AI SfaNI Tsp45I