(Linear) (Six-base) MAPSORT of: KIEE3960.seq Check: 2032 from: 1 to: 1686 KIEE3960.orf With 182 enzymes: SgfI * March 22, 2012 16:59 .. AccI GT'mk_AC Cuts at: 0 1665 1686 Size: 1665 21 Acc65I G'GTAC_C Cuts at: 0 1544 1686 Size: 1544 142 AclI AA'CG_TT Cuts at: 0 1259 1686 Size: 1259 427 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1686 Size: 907 779 AflIII A'CryG_T Cuts at: 0 151 1686 Size: 151 1535 AleI CACnn'nnGTG Cuts at: 0 517 668 1686 Size: 517 151 1018 Fragments arranged by size: 1018 517 151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1161 1686 Size: 1161 525 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1244 1276 1686 Size: 1244 32 410 Fragments arranged by size: 1244 410 32 AlwNI CAG_nnn'CTG Cuts at: 0 396 960 1686 Size: 396 564 726 Fragments arranged by size: 726 564 396 ApaI G_GGCC'C Cuts at: 0 912 1686 Size: 912 774 ApoI r'AATT_y Cuts at: 0 921 1160 1609 1686 Size: 921 239 449 77 Fragments arranged by size: 921 449 239 77 AvaI C'yCGr_G Cuts at: 0 1181 1686 Size: 1181 505 AvrII C'CTAG_G Cuts at: 0 1548 1686 Size: 1548 138 BanI G'GyrC_C Cuts at: 0 1528 1544 1686 Size: 1528 16 142 Fragments arranged by size: 1528 142 16 BanII G_rGCy'C Cuts at: 0 173 722 912 1182 1417 1686 Size: 173 549 190 270 235 269 Fragments arranged by size: 549 270 269 235 190 173 BbsI GAAGACnn'nnnn_ Cuts at: 0 905 1686 Size: 905 781 Bme1580I G_kGCm'C Cuts at: 0 795 912 1686 Size: 795 117 774 Fragments arranged by size: 795 774 117 BmrI ACTGGGnnnn_n' Cuts at: 0 1294 1686 Size: 1294 392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 192 1686 Size: 192 1494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 187 388 775 781 998 1118 1473 Size: 178 9 201 387 6 217 120 355 Cuts at: 1473 1686 Size: 213 Fragments arranged by size: 387 355 217 213 201 178 120 9 6 Bpu10I CC'TnA_GC Cuts at: 0 296 1060 1686 Size: 296 764 626 Fragments arranged by size: 764 626 296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 1686 Size: 226 1460 BsaAI yAC'GTr Cuts at: 0 152 379 744 1686 Size: 152 227 365 942 Fragments arranged by size: 942 365 227 152 BsaBI GATnn'nnATC Cuts at: 0 271 1030 1686 Size: 271 759 656 Fragments arranged by size: 759 656 271 BsaHI Gr'CG_yC Cuts at: 0 1370 1686 Size: 1370 316 BsaWI w'CCGG_w Cuts at: 0 830 1686 Size: 830 856 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1244 1274 1686 Size: 1244 30 412 Fragments arranged by size: 1244 412 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 712 880 1265 1452 1674 1686 Size: 712 168 385 187 222 12 Fragments arranged by size: 712 385 222 187 168 12 BseYI C'CCAG_C Cuts at: 0 24 944 1686 Size: 24 920 742 Fragments arranged by size: 920 742 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 1429 1461 1686 Size: 41 1388 32 225 Fragments arranged by size: 1388 225 41 32 BsiHKAI G_wGCw'C Cuts at: 0 722 998 1686 Size: 722 276 688 Fragments arranged by size: 722 688 276 BsmI GAATG_Cn' Cuts at: 0 1633 1686 Size: 1633 53 Bsp1286I G_dGCh'C Cuts at: 0 173 722 795 912 998 1182 1417 1686 Size: 173 549 73 117 86 184 235 269 Fragments arranged by size: 549 269 235 184 173 117 86 73 BspEI T'CCGG_A Cuts at: 0 830 1686 Size: 830 856 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 314 454 1686 Size: 314 140 1232 Fragments arranged by size: 1232 314 140 BsrBI CCG'CTC Cuts at: 0 1510 1686 Size: 1510 176 BsrDI GCAATG_nn' Cuts at: 0 690 1686 Size: 690 996 BsrFI r'CCGG_y Cuts at: 0 620 1686 Size: 620 1066 BstAPI GCAn_nnn'nTGC Cuts at: 0 1484 1686 Size: 1484 202 BstXI CCAn_nnnn'nTGG Cuts at: 0 156 700 1044 1150 1686 Size: 156 544 344 106 536 Fragments arranged by size: 544 536 344 156 106 BstYI r'GATC_y Cuts at: 0 773 1025 1686 Size: 773 252 661 Fragments arranged by size: 773 661 252 Bsu36I CC'TnA_GG Cuts at: 0 1127 1686 Size: 1127 559 BtgI C'CryG_G Cuts at: 0 311 515 1149 1560 1686 Size: 311 204 634 411 126 Fragments arranged by size: 634 411 311 204 126 BtsI GCAGTG_nn' Cuts at: 0 81 821 1470 1686 Size: 81 740 649 216 Fragments arranged by size: 740 649 216 81 DraIII CAC_nnn'GTG Cuts at: 0 152 1686 Size: 152 1534 DrdI GACnn_nn'nnGTC Cuts at: 0 1300 1686 Size: 1300 386 EaeI y'GGCC_r Cuts at: 0 347 1557 1686 Size: 347 1210 129 Fragments arranged by size: 1210 347 129 EarI CTCTTCn'nnn_ Cuts at: 0 427 1686 Size: 427 1259 EcoICRI GAG'CTC Cuts at: 0 720 1686 Size: 720 966 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 187 388 775 781 907 998 1118 Size: 178 9 201 387 6 126 91 120 Cuts at: 1118 1473 1686 Size: 355 213 Fragments arranged by size: 387 355 213 201 178 126 120 91 9 6 EcoO109I rG'GnC_Cy Cuts at: 0 393 750 1686 Size: 393 357 936 Fragments arranged by size: 936 393 357 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1134 1686 Size: 1134 552 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 1665 1686 Size: 199 32 1434 21 Fragments arranged by size: 1434 199 32 21 KpnI G_GTAC'C Cuts at: 0 1548 1686 Size: 1548 138 MscI TGG'CCA Cuts at: 0 1559 1686 Size: 1559 127 MslI CAynn'nnrTG Cuts at: 0 517 668 698 1686 Size: 517 151 30 988 Fragments arranged by size: 988 517 151 30 MspA1I CmG'CkG Cuts at: 0 165 823 894 933 948 1010 1493 1686 Size: 165 658 71 39 15 62 483 193 Fragments arranged by size: 658 483 193 165 71 62 39 15 NcoI C'CATG_G Cuts at: 0 311 515 1560 1686 Size: 311 204 1045 126 Fragments arranged by size: 1045 311 204 126 NspI r_CATG'y Cuts at: 0 972 1408 1686 Size: 972 436 278 Fragments arranged by size: 972 436 278 PflMI CCAn_nnn'nTGG Cuts at: 0 396 516 1686 Size: 396 120 1170 Fragments arranged by size: 1170 396 120 PfoI T'CCnGG_A Cuts at: 0 233 1095 1316 1686 Size: 233 862 221 370 Fragments arranged by size: 862 370 233 221 PmlI CAC'GTG Cuts at: 0 152 744 1686 Size: 152 592 942 Fragments arranged by size: 942 592 152 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1244 1276 1686 Size: 1244 32 410 Fragments arranged by size: 1244 410 32 PspOMI G'GGCC_C Cuts at: 0 908 1686 Size: 908 778 PstI C_TGCA'G Cuts at: 0 260 588 932 938 1686 Size: 260 328 344 6 748 Fragments arranged by size: 748 344 328 260 6 PvuII CAG'CTG Cuts at: 0 165 823 894 933 948 1686 Size: 165 658 71 39 15 738 Fragments arranged by size: 738 658 165 71 39 15 SacI G_AGCT'C Cuts at: 0 722 1686 Size: 722 964 SfcI C'TryA_G Cuts at: 0 256 543 584 928 934 1686 Size: 256 287 41 344 6 752 Fragments arranged by size: 752 344 287 256 41 6 SmaI CCC'GGG Cuts at: 0 1183 1686 Size: 1183 503 SmlI C'TyrA_G Cuts at: 0 241 1686 Size: 241 1445 SpeI A'CTAG_T Cuts at: 0 46 1686 Size: 46 1640 SspI AAT'ATT Cuts at: 0 230 1686 Size: 230 1456 StyI C'CwwG_G Cuts at: 0 311 515 1358 1548 1560 1686 Size: 311 204 843 190 12 126 Fragments arranged by size: 843 311 204 190 126 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 304 1592 1686 Size: 304 1288 94 Fragments arranged by size: 1288 304 94 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 697 729 1686 Size: 697 32 957 Fragments arranged by size: 957 697 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 342 1044 1686 Size: 342 702 642 Fragments arranged by size: 702 642 342 XmaI C'CCGG_G Cuts at: 0 1181 1686 Size: 1181 505 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I KpnI MscI MslI MspA1I NcoI NspI PflMI PfoI PmlI PpiI PspOMI PstI PvuII SacI SfcI SmaI SmlI SpeI SspI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI