(Circular) (Six-base) MAPSORT of: pFN21AE1961.seq Check: 6485 from: 1 to: 6277 pFN21AE1961 6277 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2752 2752 Size: 6277 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2937 3620 1937 Size: 1000 683 4594 Fragments arranged by size: 4594 1000 683 Acc65I G'GTAC_C Cuts at: 3333 3598 3333 Size: 265 6012 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3451 3534 4340 5724 5992 1632 Size: 1819 83 806 1384 268 1917 Fragments arranged by size: 1917 1819 1384 806 268 83 AflII C'TTAA_G Cuts at: 820 1017 3452 820 Size: 197 2435 3645 Fragments arranged by size: 3645 2435 197 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 3283 4020 5592 3283 Size: 737 1572 3968 Fragments arranged by size: 3968 1572 737 ApaI G_GGCC'C Cuts at: 3350 3350 Size: 6277 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2900 3492 3542 3586 3812 3964 6073 1493 Size: 1407 592 50 44 226 152 2109 1697 Fragments arranged by size: 2109 1697 1407 592 226 152 50 44 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2637 2979 3602 1870 Size: 73 694 342 623 4545 Fragments arranged by size: 4545 694 623 342 73 AvrII C'CTAG_G Cuts at: 3173 3173 Size: 6277 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2800 2833 3325 3358 2800 Size: 33 492 33 5719 Fragments arranged by size: 5719 492 33 33 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 3333 3598 5053 618 Size: 325 217 627 459 1087 265 1455 1842 Fragments arranged by size: 1842 1455 1087 627 459 325 265 217 BanII G_rGCy'C Cuts at: 1391 2169 2460 2733 3217 3350 3596 1391 Size: 778 291 273 484 133 246 4072 Fragments arranged by size: 4072 778 484 291 273 246 133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5306 BbvCI CC'TCA_GC Cuts at: 3135 3135 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 2828 4169 5379 2002 Size: 826 1341 1210 2900 Fragments arranged by size: 2900 1341 1210 826 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3188 4888 136 Size: 107 122 71 631 605 1516 1700 1525 Fragments arranged by size: 1700 1525 1516 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 2512 2566 3350 4338 5494 1790 Size: 722 54 784 988 1156 2573 Fragments arranged by size: 2573 1156 988 784 722 54 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2227 2949 4008 Size: 416 53 83 399 24 810 722 1059 Cuts at: 4008 4966 442 Size: 958 1753 Fragments arranged by size: 1753 1059 958 810 722 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2450 2747 3113 3203 4937 1239 Size: 942 269 297 366 90 1734 2579 Fragments arranged by size: 2579 1734 942 366 297 269 90 Bpu10I CC'TnA_GC Cuts at: 2732 3135 2732 Size: 403 5874 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3001 4402 5267 5565 3001 Size: 1401 865 298 3713 Fragments arranged by size: 3713 1401 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 4940 882 Size: 617 720 2721 2219 Fragments arranged by size: 2721 2219 720 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2643 4710 5108 5382 5529 5940 2643 Size: 2067 398 274 147 411 2980 Fragments arranged by size: 2980 2067 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 2684 3152 1186 Size: 1288 210 468 4311 Fragments arranged by size: 4311 1288 468 210 BseYI C'CCAG_C Cuts at: 5480 5976 5480 Size: 496 5781 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5704 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2733 4338 4423 5494 2259 Size: 474 1605 85 1071 3042 Fragments arranged by size: 3042 1605 1071 474 85 BsmI GAATG_Cn' Cuts at: 2474 3788 3881 2474 Size: 1314 93 4870 Fragments arranged by size: 4870 1314 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3131 3131 Size: 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2259 2460 2512 2566 2733 3217 Size: 399 379 90 201 52 54 167 484 Cuts at: 3217 3350 3596 4338 4423 5494 1391 Size: 133 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 379 246 201 167 133 90 85 54 52 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2752 3237 3632 844 Size: 1908 485 395 3489 Fragments arranged by size: 3489 1908 485 395 BsrBI CCG'CTC Cuts at: 1394 1823 2772 4164 1394 Size: 429 949 1392 3507 Fragments arranged by size: 3507 1392 949 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2277 4772 4946 65 Size: 1183 1029 2495 174 1396 Fragments arranged by size: 2495 1396 1183 1029 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 2054 5081 2054 Size: 3027 3250 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 2920 2920 Size: 6277 BstYI r'GATC_y Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272 Size: 1635 367 385 17 1452 86 12 346 Cuts at: 6272 1972 Size: 1977 Fragments arranged by size: 1977 1635 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2232 2469 2543 3204 513 Size: 552 939 228 237 74 661 3586 Fragments arranged by size: 3586 939 661 552 237 228 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 3102 3793 4587 4607 3102 Size: 691 794 20 4772 Fragments arranged by size: 4772 794 691 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3069 3104 529 Size: 35 2505 35 3702 Fragments arranged by size: 3702 2505 35 35 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2712 2922 Size: 54 1425 50 275 18 405 477 210 Cuts at: 2922 2971 3207 3724 4613 6242 8 Size: 49 236 517 889 1629 43 Fragments arranged by size: 1629 1425 889 517 477 405 275 236 210 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2702 3036 3096 3510 4207 1353 Size: 1349 334 60 414 697 3423 Fragments arranged by size: 3423 1349 697 414 334 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2731 2731 Size: 6277 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2450 2747 3113 3203 3451 3534 Size: 393 549 269 297 366 90 248 83 Cuts at: 3534 4340 4937 5724 5992 1239 Size: 806 597 787 268 1524 Fragments arranged by size: 1524 806 787 597 549 393 366 297 269 268 248 90 83 EcoO109I rG'GnC_Cy Cuts at: 2502 3347 2502 Size: 845 5432 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 3233 4783 3233 Size: 1550 4727 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2771 2803 3518 Size: 32 135 32 428 32 739 32 715 Cuts at: 3518 3550 4918 4950 4992 5024 1373 Size: 32 1368 32 42 32 2626 Fragments arranged by size: 2626 1368 739 715 428 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3621 3867 669 Size: 988 281 1683 246 3079 Fragments arranged by size: 3079 1683 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3337 3602 3337 Size: 265 6012 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5391 5575 1305 Size: 4086 184 2007 Fragments arranged by size: 4086 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 2973 3209 10 Size: 54 1425 343 882 259 236 3078 Fragments arranged by size: 3078 1425 882 343 259 236 54 MslI CAynn'nnrTG Cuts at: 518 1166 4235 4594 4753 518 Size: 648 3069 359 159 2042 Fragments arranged by size: 3069 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2740 2999 3375 3693 4370 5518 5763 1606 Size: 1134 259 376 318 677 1148 245 2120 Fragments arranged by size: 2120 1148 1134 677 376 318 259 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2469 3204 513 Size: 552 939 465 735 3586 Fragments arranged by size: 3586 939 735 552 465 NdeI CA'TA_TG Cuts at: 387 2919 387 Size: 2532 3745 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2542 3635 5180 1132 Size: 97 1313 1093 1545 2229 Fragments arranged by size: 2229 1545 1313 1093 97 PasI CC'CwG_GG Cuts at: 1348 3155 1348 Size: 1807 4470 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 3262 1108 Size: 1602 552 4123 Fragments arranged by size: 4123 1602 552 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3321 3353 4346 4378 5859 5891 3321 Size: 32 993 32 1481 32 3707 Fragments arranged by size: 3707 1481 993 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2502 2502 Size: 6277 PshAI GACnn'nnGTC Cuts at: 1613 2098 1613 Size: 485 5792 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PspOMI G'GGCC_C Cuts at: 3346 3346 Size: 6277 PstI C_TGCA'G Cuts at: 830 2745 2763 2998 3279 3629 830 Size: 1915 18 235 281 350 3478 Fragments arranged by size: 3478 1915 350 281 235 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2740 2999 2740 Size: 259 6018 SacI G_AGCT'C Cuts at: 2733 2733 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2741 2759 2797 2994 3275 3625 4760 Size: 219 1696 18 38 197 281 350 1135 Cuts at: 4760 5441 5632 826 Size: 681 191 1471 Fragments arranged by size: 1696 1471 1135 681 350 281 219 197 191 38 18 SfiI GGCCn_nnn'nGGCC Cuts at: 3188 3188 Size: 6277 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 2981 3604 1872 Size: 1109 623 4545 Fragments arranged by size: 4545 1109 623 SmlI C'TyrA_G Cuts at: 820 1017 1943 3016 3452 4381 5282 5544 820 Size: 197 926 1073 436 929 901 262 1553 Fragments arranged by size: 1553 1073 929 926 901 436 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 3107 3320 1517 Size: 1590 213 4474 Fragments arranged by size: 4474 1590 213 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 3173 3204 3315 3716 513 Size: 552 939 465 704 31 111 401 3074 Fragments arranged by size: 3074 939 704 552 465 401 111 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4304 4321 4474 4659 5104 325 Size: 1255 2724 17 153 185 445 1498 Fragments arranged by size: 2724 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4523 6235 96 Size: 274 80 33 51 1443 2546 1712 138 Fragments arranged by size: 2546 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2249 2281 2502 2534 6237 6269 2249 Size: 32 221 32 3703 32 2257 Fragments arranged by size: 3703 2257 221 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2722 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 2979 3602 1870 Size: 1109 623 4545 Fragments arranged by size: 4545 1109 623 XmnI GAAnn'nnTTC Cuts at: 2182 4406 2182 Size: 2224 4053 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI