################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MamI MflI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 184 GAATTC [GA]^AATT[CT] AluI 731 AGCT AG^CT AlwI 174 GATCC GGATC(4/5) AlwNI 469 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 178 CCTGAGG CC^T[ACGT]AGG ApoI 184 GAATTC [GA]^AATT[CT] AvaI 8 CCCGGG C^[CT]CG[GA]G AvrII 66 CCTAGG C^CTAGG BbrPI 664 CACGTG CAC^GTG BglI 31 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 66 CCTAGG C^CTAGG BsaAI 664 CACGTG [CT]AC^GT[GA] BsaI 292 GAGACC GGTCTC(1/5) BsgI 607 GTGCAG GTGCAG(16/14) BsoBI 8 CCCGGG C^[CT]CG[GA]G Bsu36I 178 CCTGAGG CC^T[ACGT]AGG DdeI 179 CTGAG C^T[ACGT]AG DpnII 174 GATC ^GATC DraIII 664 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 178 CCTGAGG CC^T[ACGT]AGG EcoRI 184 GAATTC G^AATTC EcoT14I 66 CCTAGG C^C[AT][AT]GG HaeII 485 AGCGCC [GA]GCGC^[CT] HaeIII 39 GGCC GG^CC HgaI 621 GCGTC GACGC(5/10) HinfI 582 GAATC G^A[ACGT]TC MaeII 665 ACGT A^CGT MboI 174 GATC ^GATC MseI 338 TTAA T^TAA MslI 519 CATTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 35 CAGCGG C[AC]G^C[GT]G NdeII 174 GATC ^GATC PmaCI 664 CACGTG CAC^GTG PmlI 664 CACGTG CAC^GTG Sau3AI 174 GATC ^GATC ScaI 312 AGTACT AGT^ACT SmaI 8 CCCGGG CCC^GGG SmlI 749 CTCAAG C^T[CT][GA]AG StyI 66 CCTAGG C^C[AT][AT]GG TaqI 624 TCGA T^CGA TfiI 582 GAATC G^A[AT]TC Tru9I 338 TTAA T^TAA Tsp45I 462 GTCAC ^GT[GC]AC TthHB8I 624 TCGA T^CGA XmaCI 8 CCCGGG C^CCGGG XmaI 8 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### BsiYI 142 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 205 CCTAAAACTGG BslI 142 CCTGAATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 205 CCTAAAACTGG BsmAI 292 GAGAC GTCTC(1/5) 700 GAGAC BsmI 54 GAATGC GAATGC(1/-1) 441 GAATGC BsrDI 519 CATTGC GCAATG(2/0) 717 CATTGC BstAPI 361 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 663 GCACGTGGTGC BstF5I 426 CATCC GGATG(2/0) 435 CATCC BstNI 73 CCAGG CC^[AT]GG 588 CCTGG CfoI 287 GCGC GCG^C 486 GCGC DraII 94 AGGACCT [GA]G^G[ACGT]CC[CT] 222 AGGACCC EarI 137 CTCTTC CTCTTC(1/4) 406 GAAGAG EcoO109I 94 AGGACCT [GA]G^G[ACGT]CC[CT] 222 AGGACCC EcoRII 73 CCAGG ^CC[AT]GG 588 CCTGG FokI 426 CATCC GGATG(9/13) 435 CATCC HapII 9 CCGG C^CGG 546 CCGG HhaI 287 GCGC GCG^C 486 GCGC HinP1I 287 GCGC G^CGC 486 GCGC HpaII 9 CCGG C^CGG 546 CCGG Ksp632I 137 CTCTTC CTCTTC(1/4) 406 GAAGAG MfeI 446 CAATTG C^AATTG 704 CAATTG MspI 9 CCGG C^CGG 546 CCGG MunI 446 CAATTG C^AATTG 704 CAATTG MvaI 73 CCAGG CC^[AT]GG 588 CCTGG NlaIII 615 CATG CATG^ 642 CATG PpuMI 94 AGGACCT [GA]G^G[AT]CC[CT] 222 AGGACCC SfaNI 618 GATGC GCATC(5/9) 677 GCATC TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^ 473 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AfaI AvaII BbvI BcnI BfaI BsaJI BsmFI BsrI BssKI Cac8I Cfr13I Fnu4HI ItaI MaeI MaeIII MboII MnlI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI Tsp509I