(Linear) (Six-base) MAPSORT of: KIEE6282.seq Check: 5865 from: 1 to: 768 KIEE6282.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 768 Size: 70 698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 410 768 Size: 410 358 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 143 175 768 Size: 143 32 593 Fragments arranged by size: 593 143 32 AlwNI CAG_nnn'CTG Cuts at: 0 474 768 Size: 474 294 ApoI r'AATT_y Cuts at: 0 184 768 Size: 184 584 AvaI C'yCGr_G Cuts at: 0 8 768 Size: 8 760 AvrII C'CTAG_G Cuts at: 0 66 768 Size: 66 702 BglI GCCn_nnn'nGGC Cuts at: 0 37 768 Size: 37 731 BmrI ACTGGGnnnn_n' Cuts at: 0 251 768 Size: 251 517 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 734 768 Size: 734 34 BsaI GGTCTCn'nnnn_ Cuts at: 0 286 768 Size: 286 482 BsaAI yAC'GTr Cuts at: 0 666 768 Size: 666 102 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 143 173 768 Size: 143 30 595 Fragments arranged by size: 595 143 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 628 768 Size: 628 140 BsmI GAATG_Cn' Cuts at: 0 60 447 768 Size: 60 387 321 Fragments arranged by size: 387 321 60 BsrDI GCAATG_nn' Cuts at: 0 518 716 768 Size: 518 198 52 Fragments arranged by size: 518 198 52 BstAPI GCAn_nnn'nTGC Cuts at: 0 367 669 768 Size: 367 302 99 Fragments arranged by size: 367 302 99 Bsu36I CC'TnA_GG Cuts at: 0 179 768 Size: 179 589 BtsI GCAGTG_nn' Cuts at: 0 299 768 Size: 299 469 DraIII CAC_nnn'GTG Cuts at: 0 669 768 Size: 669 99 EarI CTCTTCn'nnn_ Cuts at: 0 143 401 768 Size: 143 258 367 Fragments arranged by size: 367 258 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 12 768 Size: 12 756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 768 Size: 70 698 EcoO109I rG'GnC_Cy Cuts at: 0 95 223 768 Size: 95 128 545 Fragments arranged by size: 545 128 95 EcoRI G'AATT_C Cuts at: 0 184 768 Size: 184 584 HaeII r_GCGC'y Cuts at: 0 489 768 Size: 489 279 MfeI C'AATT_G Cuts at: 0 446 704 768 Size: 446 258 64 Fragments arranged by size: 446 258 64 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 453 768 Size: 453 315 MslI CAynn'nnrTG Cuts at: 0 523 768 Size: 523 245 MspA1I CmG'CkG Cuts at: 0 37 768 Size: 37 731 PmlI CAC'GTG Cuts at: 0 666 768 Size: 666 102 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 143 175 768 Size: 143 32 593 Fragments arranged by size: 593 143 32 PpuMI rG'GwC_Cy Cuts at: 0 95 223 768 Size: 95 128 545 Fragments arranged by size: 545 128 95 ScaI AGT'ACT Cuts at: 0 314 768 Size: 314 454 SmaI CCC'GGG Cuts at: 0 10 768 Size: 10 758 SmlI C'TyrA_G Cuts at: 0 749 768 Size: 749 19 StyI C'CwwG_G Cuts at: 0 66 768 Size: 66 702 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 642 768 Size: 642 126 TatI w'GTAC_w Cuts at: 0 231 312 768 Size: 231 81 456 Fragments arranged by size: 456 231 81 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 107 139 768 Size: 107 32 629 Fragments arranged by size: 629 107 32 XmaI C'CCGG_G Cuts at: 0 8 768 Size: 8 760 Enzymes that do cut: AcuI AlfI AloI AlwNI ApoI AvaI AvrII BglI BmrI BpuEI BsaI BsaAI BsaXI BsgI BsmI BsrDI BstAPI Bsu36I BtsI DraIII EarI EciI Eco57MI EcoO109I EcoRI HaeII MfeI MmeI MslI MspA1I PmlI PpiI PpuMI ScaI SmaI SmlI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI