(Linear) (Six-base) MAPSORT of: KIEE5255.seq Check: 5131 from: 1 to: 519 KIEE5255.orf With 182 enzymes: SgfI * December 5, 2014 13:00 .. AlwNI CAG_nnn'CTG Cuts at: 0 63 519 Size: 63 456 ApaLI G'TGCA_C Cuts at: 0 312 502 519 Size: 312 190 17 Fragments arranged by size: 312 190 17 BanI G'GyrC_C Cuts at: 0 141 519 Size: 141 378 BanII G_rGCy'C Cuts at: 0 46 519 Size: 46 473 BbeI G_GCGC'C Cuts at: 0 145 519 Size: 145 374 BbsI GAAGACnn'nnnn_ Cuts at: 0 281 519 Size: 281 238 BclI T'GATC_A Cuts at: 0 119 519 Size: 119 400 BlpI GC'TnA_GC Cuts at: 0 404 519 Size: 404 115 Bme1580I G_kGCm'C Cuts at: 0 316 506 519 Size: 316 190 13 Fragments arranged by size: 316 190 13 BmgBI CAC'GTC Cuts at: 0 202 519 Size: 202 317 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 392 519 Size: 392 127 BsaHI Gr'CG_yC Cuts at: 0 142 519 Size: 142 377 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 130 160 519 Size: 130 30 359 Fragments arranged by size: 359 130 30 BseYI C'CCAG_C Cuts at: 0 213 392 519 Size: 213 179 127 Fragments arranged by size: 213 179 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 519 Size: 32 487 BsiHKAI G_wGCw'C Cuts at: 0 316 506 519 Size: 316 190 13 Fragments arranged by size: 316 190 13 Bsp1286I G_dGCh'C Cuts at: 0 46 316 506 519 Size: 46 270 190 13 Fragments arranged by size: 270 190 46 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 4 519 Size: 4 515 BsrBI CCG'CTC Cuts at: 0 164 519 Size: 164 355 BstEII G'GTnAC_C Cuts at: 0 16 28 519 Size: 16 12 491 Fragments arranged by size: 491 16 12 BtgI C'CryG_G Cuts at: 0 345 519 Size: 345 174 BtsI GCAGTG_nn' Cuts at: 0 86 506 519 Size: 86 420 13 Fragments arranged by size: 420 86 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 392 519 Size: 392 127 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 519 Size: 55 464 EcoO109I rG'GnC_Cy Cuts at: 0 350 519 Size: 350 169 EcoRV GAT'ATC Cuts at: 0 380 519 Size: 380 139 HaeII r_GCGC'y Cuts at: 0 145 519 Size: 145 374 KasI G'GCGC_C Cuts at: 0 141 519 Size: 141 378 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 282 519 Size: 282 237 MslI CAynn'nnrTG Cuts at: 0 493 519 Size: 493 26 MspA1I CmG'CkG Cuts at: 0 213 347 519 Size: 213 134 172 Fragments arranged by size: 213 172 134 NarI GG'CG_CC Cuts at: 0 142 519 Size: 142 377 NspI r_CATG'y Cuts at: 0 370 519 Size: 370 149 PpuMI rG'GwC_Cy Cuts at: 0 350 519 Size: 350 169 SacII CC_GC'GG Cuts at: 0 348 519 Size: 348 171 SanDI GG'GwC_CC Cuts at: 0 350 519 Size: 350 169 SfoI GGC'GCC Cuts at: 0 143 519 Size: 143 376 SphI G_CATG'C Cuts at: 0 370 519 Size: 370 149 StuI AGG'CCT Cuts at: 0 184 519 Size: 184 335 StyI C'CwwG_G Cuts at: 0 257 519 Size: 257 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 229 519 Size: 229 290 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 65 97 191 223 414 446 519 Size: 65 32 94 32 191 32 73 Fragments arranged by size: 191 94 73 65 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 354 519 Size: 354 165 XbaI T'CTAG_A Cuts at: 0 444 519 Size: 444 75 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 481 519 Size: 481 38 Enzymes that do cut: AlwNI ApaLI BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI BpmI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BstEII BtgI BtsI Eco57MI EcoNI EcoO109I EcoRV HaeII KasI MmeI MslI MspA1I NarI NspI PpuMI SacII SanDI SfoI SphI StuI StyI TaqII TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI XhoI XmaI XmnI ZraI