(Circular) (Six-base) MAPSORT of: pFN21AB8821.seq Check: 6614 from: 1 to: 5857 pFN21AB8821 5857 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2709 2709 Size: 5857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 3012 4756 5733 1203 Size: 1809 1744 977 1327 Fragments arranged by size: 1809 1744 1327 977 AgeI A'CCGG_T Cuts at: 2042 4688 2042 Size: 2646 3211 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 2905 3101 1166 Size: 1739 196 3922 Fragments arranged by size: 3922 1739 196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 2244 2458 2806 2902 3600 5172 2244 Size: 214 348 96 698 1572 2929 Fragments arranged by size: 2929 1572 698 348 214 96 ApaI G_GGCC'C Cuts at: 2771 2771 Size: 5857 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2276 2433 2746 3182 1870 Size: 73 333 157 313 436 4545 Fragments arranged by size: 4545 436 333 313 157 73 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2437 2469 2542 2555 2750 Size: 325 217 627 650 32 73 13 195 Cuts at: 2750 2777 2941 3131 3178 4633 618 Size: 27 164 190 47 1455 1842 Fragments arranged by size: 1842 1455 650 627 325 217 195 190 164 73 47 32 27 13 BanII G_rGCy'C Cuts at: 1391 2283 2307 2771 3176 1391 Size: 892 24 464 405 4072 Fragments arranged by size: 4072 892 464 405 24 BbeI G_GCGC'C Cuts at: 2441 2473 2559 2754 2781 2441 Size: 32 86 195 27 5517 Fragments arranged by size: 5517 195 86 32 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2872 928 Size: 971 973 3913 Fragments arranged by size: 3913 973 971 BbvCI CC'TCA_GC Cuts at: 2237 2237 Size: 5857 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2600 2775 4468 Size: 107 122 71 631 605 928 175 1693 Cuts at: 4468 136 Size: 1525 Fragments arranged by size: 1693 1525 928 631 605 175 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 2141 2545 2771 2836 2946 3108 3134 3918 Size: 351 404 226 65 110 162 26 784 Cuts at: 3918 5074 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 784 404 351 226 162 110 65 26 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 3137 3163 Size: 416 53 83 399 24 909 811 26 Cuts at: 3163 3588 4546 442 Size: 425 958 1753 Fragments arranged by size: 1753 958 909 811 425 416 399 83 53 26 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4517 1239 Size: 3278 2579 Bpu10I CC'TnA_GC Cuts at: 2237 2237 Size: 5857 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3015 3648 493 Size: 711 1811 633 2702 Fragments arranged by size: 2702 1811 711 633 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2438 2470 2556 2751 Size: 53 83 186 936 905 32 86 195 Cuts at: 2751 2778 2842 3063 3664 4046 4681 275 Size: 27 64 221 601 382 635 1451 Fragments arranged by size: 1451 936 905 635 601 382 221 195 186 86 83 64 53 32 27 BsaWI w'CCGG_w Cuts at: 2042 4290 4688 4962 5109 5520 2042 Size: 2248 398 274 147 411 2379 Fragments arranged by size: 2379 2248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 1178 Size: 30 165 30 600 30 5002 Fragments arranged by size: 5002 600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2005 2008 2023 2090 2378 3041 1186 Size: 819 3 15 67 288 663 4002 Fragments arranged by size: 4002 819 663 288 67 15 3 BseYI C'CCAG_C Cuts at: 2327 2362 2754 2799 5060 5556 2327 Size: 35 392 45 2261 496 2628 Fragments arranged by size: 2628 2261 496 392 45 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2615 2690 2804 2975 1163 Size: 573 879 75 114 171 4045 Fragments arranged by size: 4045 879 573 171 114 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3081 3307 4068 5096 1540 Size: 275 186 1080 226 761 1028 2301 Fragments arranged by size: 2301 1080 1028 761 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2277 2691 3918 4003 5074 2277 Size: 414 1227 85 1071 3060 Fragments arranged by size: 3060 1227 1071 414 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 BsmBI CGTCTCn'nnnn_ Cuts at: 2849 2849 Size: 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2277 2283 2307 2545 2691 2771 Size: 399 351 136 6 24 238 146 80 Cuts at: 2771 2836 2946 3108 3134 3176 3918 4003 5074 Size: 65 110 162 26 42 742 85 1071 Cuts at: 5074 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 351 238 162 146 136 110 85 80 65 42 26 24 6 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2430 2709 3212 844 Size: 1586 279 503 3489 Fragments arranged by size: 3489 1586 503 279 BsrBI CCG'CTC Cuts at: 1394 1823 3039 3744 1394 Size: 429 1216 705 3507 Fragments arranged by size: 3507 1216 705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2091 2291 2938 4501 4688 1700 Size: 110 232 49 200 647 1563 187 2869 Fragments arranged by size: 2869 1563 647 232 200 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 2168 2402 2519 3917 4929 2168 Size: 234 117 1398 1012 3096 Fragments arranged by size: 3096 1398 1012 234 117 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 794 Size: 1919 3938 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2907 2155 Size: 752 5105 BstYI r'GATC_y Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852 Size: 1215 367 385 17 1452 86 12 346 Cuts at: 5852 1972 Size: 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2546 2598 2825 3141 513 Size: 552 1481 52 227 316 3229 Fragments arranged by size: 3229 1481 552 316 227 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 2395 2632 3117 3373 4167 4187 2395 Size: 237 485 256 794 20 4065 Fragments arranged by size: 4065 794 485 256 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2240 4864 809 Size: 507 924 2624 1802 Fragments arranged by size: 2624 1802 924 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2145 2592 Size: 54 1425 50 275 18 297 18 447 Cuts at: 2592 2889 2919 2961 3078 3304 4193 5822 8 Size: 297 30 42 117 226 889 1629 43 Fragments arranged by size: 1629 1425 889 447 297 297 275 226 117 54 50 43 42 30 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3078 3304 1537 Size: 275 1266 226 4090 Fragments arranged by size: 4090 1266 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 3787 1353 Size: 714 1720 3423 Fragments arranged by size: 3423 1720 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3023 4444 4818 4964 387 Size: 1064 138 1434 1421 374 146 1280 Fragments arranged by size: 1434 1421 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3920 4517 5304 5572 1239 Size: 393 2288 597 787 268 1524 Fragments arranged by size: 2288 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2768 2768 Size: 5857 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2328 2441 2459 2473 2495 2507 2559 2588 Size: 650 113 18 14 22 12 52 29 Cuts at: 2588 2606 2754 2781 5004 1678 Size: 18 148 27 2223 2531 Fragments arranged by size: 2531 2223 650 148 113 52 29 27 22 18 18 14 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373 Size: 32 135 32 2926 32 42 32 2626 Fragments arranged by size: 2926 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KasI G'GCGC_C Cuts at: 2437 2469 2555 2750 2777 2437 Size: 32 86 195 27 5517 Fragments arranged by size: 5517 195 86 32 27 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4971 5155 1305 Size: 3666 184 2007 Fragments arranged by size: 3666 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 2147 2891 2921 10 Size: 54 1425 343 297 18 744 30 2946 Fragments arranged by size: 2946 1425 744 343 297 54 30 18 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2905 3101 3815 4174 4333 518 Size: 648 936 803 196 714 359 159 2042 Fragments arranged by size: 2042 936 803 714 648 359 196 159 MspA1I CmG'CkG Cuts at: 1606 2548 2600 2827 2839 2850 2925 2967 3004 Size: 942 52 227 12 11 75 42 37 Cuts at: 3004 3083 3143 3273 3950 5098 5343 1606 Size: 79 60 130 677 1148 245 2120 Fragments arranged by size: 2120 1148 942 677 245 227 130 79 75 60 52 42 37 12 11 NaeI GCC'GGC Cuts at: 2093 2093 Size: 5857 NarI GG'CG_CC Cuts at: 2438 2470 2556 2751 2778 2438 Size: 32 86 195 27 5517 Fragments arranged by size: 5517 195 86 32 27 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NgoMIV G'CCGG_C Cuts at: 2091 2091 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3078 3304 3078 Size: 226 5631 NspI r_CATG'y Cuts at: 1132 1229 2488 3215 4760 1132 Size: 97 1259 727 1545 2229 Fragments arranged by size: 2229 1545 1259 727 97 PasI CC'CwG_GG Cuts at: 1348 2512 2538 2946 1348 Size: 1164 26 408 4259 Fragments arranged by size: 4259 1164 408 26 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PmlI CAC'GTG Cuts at: 3015 3015 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2767 2767 Size: 5857 PstI C_TGCA'G Cuts at: 830 2193 3209 830 Size: 1363 1016 3478 Fragments arranged by size: 3478 1363 1016 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2967 2967 Size: 5857 SacII CC_GC'GG Cuts at: 2549 2601 2828 3144 2549 Size: 52 227 316 5262 Fragments arranged by size: 5262 316 227 52 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2189 3205 4340 5021 5212 826 Size: 219 1144 1016 1135 681 191 1471 Fragments arranged by size: 1471 1144 1135 1016 681 219 191 SfoI GGC'GCC Cuts at: 2439 2471 2557 2752 2779 2439 Size: 32 86 195 27 5517 Fragments arranged by size: 5517 195 86 32 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 2042 1700 Size: 342 5515 SmaI CCC'GGG Cuts at: 1872 2435 2748 3184 1872 Size: 563 313 436 4545 Fragments arranged by size: 4545 563 436 313 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 2488 3215 1132 Size: 1356 727 3774 Fragments arranged by size: 3774 1356 727 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 3296 513 Size: 552 2231 3074 Fragments arranged by size: 3074 2231 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2899 3884 3901 4054 4239 4684 325 Size: 1255 1319 985 17 153 185 445 1498 Fragments arranged by size: 1498 1319 1255 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4103 5815 96 Size: 274 80 33 51 1443 2126 1712 138 Fragments arranged by size: 2126 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2364 2396 2556 2588 5817 5849 2364 Size: 32 160 32 3229 32 2372 Fragments arranged by size: 3229 2372 160 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2899 2899 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2433 2746 3182 1870 Size: 563 313 436 4545 Fragments arranged by size: 4545 563 436 313 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI