################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI Ksp632I KspI MaeII MamI MluI MluNI MroI MscI MslI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1216 GGTACC G^GTACC AccI 406 GTAGAC GT^[AC][GT]AC AfaI 1217 GTAC GT^AC AflIII 73 ACATGT A^C[GA][CT]GT ApaI 1262 GGGCCC GGGCC^C Asp718I 1216 GGTACC G^GTACC AspI 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 1216 GGTACC G^G[CT][GA]CC BciVI 1044 GGATAC GTATCC(6/5) BclI 1350 TGATCA T^GATCA BglI 1255 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1155 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1155 GCTCAGC GC^T[ACGT]AGC BsaWI 732 TCCGGT [AT]^CCGG[AT] BsmFI 1066 GTCCC GGGAC(10/14) BsmI 36 GAATGC GAATGC(1/-1) BspHI 471 TCATGA T^CATGA BspLU11I 73 ACATGT A^CATGT BssKI 26 CCTGG ^CC[ACGT]GG BssSI 899 CTCGTG CACGAG(-5/-1) BstAPI 517 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 26 CCTGG CC^[AT]GG CelII 1155 GCTCAGC GC^T[ACGT]AGC DraI 426 TTTAAA TTT^AAA DsaI 180 CCGTGG C^C[GA][CT]GG EcoNI 26 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 26 CCTGG ^CC[AT]GG EcoRV 610 GATATC GAT^ATC FauI 40 GCGGG CCCGC(4/6) FbaI 1350 TGATCA T^GATCA HapII 733 CCGG C^CGG HpaII 733 CCGG C^CGG HphI 658 TCACC GGTGA(8/7) KpnI 1216 GGTACC GGTAC^C MfeI 1208 CAATTG C^AATTG MspA1I 1346 CAGCTG C[AC]G^C[GT]G MspI 733 CCGG C^CGG MunI 1208 CAATTG C^AATTG MvaI 26 CCTGG CC^[AT]GG NspI 73 ACATGT [GA]CATG^[CT] PflFI 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 347 GAGTC GAGTC(4/5) PvuII 1346 CAGCTG CAG^CTG RcaI 471 TCATGA T^CATGA RsaI 1217 GTAC GT^AC ScrFI 26 CCTGG CC^[ACGT]GG SfaNI 290 GCATC GCATC(5/9) SspI 553 AATATT AAT^ATT TfiI 433 GATTC G^A[AT]TC Tsp45I 1380 GTCAC ^GT[GC]AC Tth111I 795 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 372 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 1125 CCGC CCGC(-3/-1) 40 GCGG AlwI 1204 GGATC GGATC(4/5) 1270 GGATC BanII 1140 GGGCTC G[GA]GC[CT]^C 1262 GGGCCC BglII 1088 AGATCT A^GATCT 1437 AGATCT BmyI 1140 GGGCTC G[AGT]GC[ACT]^C 1262 GGGCCC BpmI 1129 CTCCAG CTGGAG(16/14) 27 CTGGAG BsmAI 276 GTCTC GTCTC(1/5) 468 GTCTC Bsp1286I 1140 GGGCTC G[AGT]GC[ACT]^C 1262 GGGCCC BstF5I 145 CATCC GGATG(2/0) 987 GGATG BstYI 1088 AGATCT [GA]^GATC[CT] 1437 AGATCT DraII 1261 GGGGCCC [GA]G^G[ACGT]CC[CT] 1262 GGGCCCT EcoO109I 1261 GGGGCCC [GA]G^G[ACGT]CC[CT] 1262 GGGCCCT EcoT14I 947 CCTTGG C^C[AT][AT]GG 1266 CCTTGG FokI 145 CATCC GGATG(9/13) 987 GGATG HindIII 314 AAGCTT A^AGCTT 422 AAGCTT HinfI 347 GAGTC G^A[ACGT]TC 433 GATTC MflI 1088 AGATCT [GA]^GATC[CT] 1437 AGATCT MwoI 517 GCAACTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1255 GCCATTGGGGC PflMI 1131 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1391 CCAATTTGTGG SpeI 331 ACTAGT A^CTAGT 717 ACTAGT StyI 947 CCTTGG C^C[AT][AT]GG 1266 CCTTGG Van91I 1131 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1391 CCAATTTGTGG XhoII 1088 AGATCT [GA]^GATC[CT] 1437 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BbvI BfaI BsaJI BsiYI BslI BsrDI BsrI Cfr13I DdeI DpnII Fnu4HI HaeIII ItaI MaeI MaeIII MboI MboII MnlI MseI NdeII NlaIII NlaIV PstI Sau3AI Sau96I SfcI Tru9I TseI Tsp509I TspRI