################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AclI 278 AACGTT AA^CGTT AflIII 859 ACATGT A^C[GA][CT]GT BcnI 41 CCGGG CC^[GC]GG BseRI 199 GAGGAG GAGGAG(10/8) BsiEI 30 CGGCCG CG[GA][CT]^CG BsmAI 1114 GAGAC GTCTC(1/5) BsmI 1706 GCATTC GAATGC(1/-1) BspLU11I 859 ACATGT A^CATGT BsrFI 28 GCCGGC [GA]^CCGG[CT] BsrI 463 CCAGT ACTGG(1/-1) BstBI 1607 TTCGAA TT^CGAA BstYI 1242 AGATCC [GA]^GATC[CT] Cac8I 28 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 28 GCCGGC [GA]^CCGG[CT] Cfr13I 16 GGGCC G^G[ACGT]CC EagI 30 CGGCCG C^GGCCG EclXI 30 CGGCCG C^GGCCG Eco52I 30 CGGCCG C^GGCCG EcoRV 577 GATATC GAT^ATC FauI 50 CCCGC CCCGC(4/6) HgaI 931 GCGTC GACGC(5/10) HincII 1620 GTTAAC GT[CT]^[GA]AC HindII 1620 GTTAAC GT[CT]^[GA]AC HpaI 1620 GTTAAC GTT^AAC HphI 1420 GGTGA GGTGA(8/7) MaeII 279 ACGT A^CGT MflI 1242 AGATCC [GA]^GATC[CT] MslI 256 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 4 GCGGACTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 28 GCCGGC GCC^GGC NciI 41 CCGGG CC^[GC]GG NgoMIV 28 GCCGGC G^CCGGC NspI 859 ACATGT [GA]CATG^[CT] NspV 1607 TTCGAA TT^CGAA PflMI 1696 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 278 AACGTT AA^CGTT SapI 1450 GAAGAGC GCTCTTC(1/4) Sau96I 16 GGGCC G^G[ACGT]CC ScaI 88 AGTACT AGT^ACT SfcI 258 CTACAG C^T[GA][CT]AG SfuI 1607 TTCGAA TT^CGAA SmlI 1482 CTCAAG C^T[CT][GA]AG Van91I 1696 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1749 TCTAGA T^CTAGA XhoII 1242 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 1243 GATCC GGATC(4/5) 1385 GGATC BalI 525 TGGCCA TGG^CCA 1161 TGGCCA BanII 44 GGGCTC G[GA]GC[CT]^C 1311 GGGCTC BmyI 44 GGGCTC G[AGT]GC[ACT]^C 1311 GGGCTC BsaJI 41 CCGGGG C^C[ACGT][ACGT]GG 1543 CCTGGG Bsp1286I 44 GGGCTC G[AGT]GC[ACT]^C 1311 GGGCTC DpnII 1243 GATC ^GATC 1386 GATC HapII 29 CCGG C^CGG 41 CCGG HpaII 29 CCGG C^CGG 41 CCGG MboI 1243 GATC ^GATC 1386 GATC MluNI 525 TGGCCA TGG^CCA 1161 TGGCCA MscI 525 TGGCCA TGG^CCA 1161 TGGCCA MspI 29 CCGG C^CGG 41 CCGG NdeII 1243 GATC ^GATC 1386 GATC NlaIII 860 CATG CATG^ 1456 CATG NlaIV 45 GGCTCC GG[ACGT]^[ACGT]CC 1554 GGTTCC Sau3AI 1243 GATC ^GATC 1386 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BbvI BfaI BpmI BpuAI BsgI BsiYI BslI BspMI BssKI BstF5I BstNI DdeI EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MspA1I MvaI PleI PvuII RsaI ScrFI SfaNI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I