(Circular) (Six-base) MAPSORT of: pFN21AE2035.seq Check: 6834 from: 1 to: 6592 pFN21AE2035 6592 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3935 1937 Size: 1998 4594 Acc65I G'GTAC_C Cuts at: 3913 3913 Size: 6592 AclI AA'CG_TT Cuts at: 1563 4719 5092 1563 Size: 3156 373 3063 Fragments arranged by size: 3156 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 4655 6039 6307 1632 Size: 409 2614 1384 268 1917 Fragments arranged by size: 2614 1917 1384 409 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 2485 5491 6468 1203 Size: 1282 3006 977 1327 Fragments arranged by size: 3006 1327 1282 977 AgeI A'CCGG_T Cuts at: 5423 5423 Size: 6592 AhdI GACnn_n'nnGTC Cuts at: 5321 5321 Size: 6592 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6592 AlwNI CAG_nnn'CTG Cuts at: 3769 4335 5907 3769 Size: 566 1572 4454 Fragments arranged by size: 4454 1572 566 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 2637 2810 2846 3001 3465 3472 3616 3782 Size: 1144 173 36 155 464 7 144 166 Cuts at: 3782 3901 4127 4279 6388 1493 Size: 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1144 464 226 173 166 155 152 144 119 36 7 AseI AT'TA_AT Cuts at: 160 1032 2244 3828 5146 160 Size: 872 1212 1584 1318 1606 Fragments arranged by size: 1606 1584 1318 1212 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 3917 1870 Size: 73 1974 4545 Fragments arranged by size: 4545 1974 73 BamHI G'GATC_C Cuts at: 3922 4289 3922 Size: 367 6225 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3036 3913 5368 618 Size: 325 217 627 1249 877 1455 1842 Fragments arranged by size: 1842 1455 1249 877 627 325 217 BanII G_rGCy'C Cuts at: 1391 3911 1391 Size: 2520 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5621 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4783 4817 1903 Size: 34 2846 34 3678 Fragments arranged by size: 3678 2846 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5203 136 Size: 107 122 71 631 605 3531 1525 Fragments arranged by size: 3531 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6587 6587 Size: 6592 BlpI GC'TnA_GC Cuts at: 4009 4009 Size: 6592 Bme1580I G_kGCm'C Cuts at: 1790 2712 3039 4653 5809 1790 Size: 922 327 1614 1156 2573 Fragments arranged by size: 2573 1614 1156 922 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 3738 4323 Size: 416 53 83 399 24 1742 579 585 Cuts at: 4323 5281 442 Size: 958 1753 Fragments arranged by size: 1753 1742 958 585 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3398 5252 1239 Size: 2159 1854 2579 Fragments arranged by size: 2579 2159 1854 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2515 4717 5582 5880 2515 Size: 2202 865 298 3227 Fragments arranged by size: 3227 2202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5255 882 Size: 617 3756 2219 Fragments arranged by size: 3756 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2479 2875 4383 493 Size: 711 1275 396 1508 2702 Fragments arranged by size: 2702 1508 1275 711 396 BsaBI GATnn'nnATC Cuts at: 6586 6586 Size: 6592 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4399 4781 5416 275 Size: 53 83 186 936 2866 382 635 1451 Fragments arranged by size: 2866 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5025 5423 5697 5844 6255 5025 Size: 398 274 147 411 5362 Fragments arranged by size: 5362 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6367 Fragments arranged by size: 6367 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3072 3722 3827 1186 Size: 1886 650 105 3951 Fragments arranged by size: 3951 1886 650 105 BseYI C'CCAG_C Cuts at: 2447 5795 6291 2447 Size: 3348 496 2748 Fragments arranged by size: 3348 2748 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2290 1163 Size: 573 554 5465 Fragments arranged by size: 5465 573 554 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 4803 5831 1540 Size: 275 186 2041 761 1028 2301 Fragments arranged by size: 2301 2041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4653 4738 5809 4653 Size: 85 1071 5436 Fragments arranged by size: 5436 1071 85 BsmI GAATG_Cn' Cuts at: 2449 2981 3680 4103 4196 2449 Size: 532 699 423 93 4845 Fragments arranged by size: 4845 699 532 423 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2712 3039 3911 4653 4738 5809 1391 Size: 399 922 327 872 742 85 1071 2174 Fragments arranged by size: 2174 1071 922 872 742 399 327 85 BspEI T'CCGG_A Cuts at: 6255 6255 Size: 6592 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3271 3947 844 Size: 2427 676 3489 Fragments arranged by size: 3489 2427 676 BsrBI CCG'CTC Cuts at: 1394 1823 4479 1394 Size: 429 2656 3507 Fragments arranged by size: 3507 2656 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3229 5087 5261 65 Size: 1183 1981 1858 174 1396 Fragments arranged by size: 1981 1858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5236 5423 1700 Size: 110 3426 187 2869 Fragments arranged by size: 3426 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6592 BstBI TT'CG_AA Cuts at: 5396 5396 Size: 6592 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6592 BstXI CCAn_nnnn'nTGG Cuts at: 3727 3727 Size: 6592 BstYI r'GATC_y Cuts at: 1972 3165 3922 4289 4674 4691 6143 6229 6241 Size: 1193 757 367 385 17 1452 86 12 Cuts at: 6241 6587 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1193 757 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3483 3483 Size: 6592 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6040 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5853 Fragments arranged by size: 5853 683 56 BtsI GCAGTG_nn' Cuts at: 2307 4108 4902 4922 2307 Size: 1801 794 20 3977 Fragments arranged by size: 3977 1801 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2460 2495 529 Size: 35 1896 35 4626 Fragments arranged by size: 4626 1896 35 35 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DraIII CAC_nnn'GTG Cuts at: 3746 3746 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 4039 4928 Size: 54 1425 50 275 18 268 1941 889 Cuts at: 4928 6557 8 Size: 1629 43 Fragments arranged by size: 1941 1629 1425 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4039 1537 Size: 275 2227 4090 Fragments arranged by size: 4090 2227 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2486 2505 2613 4522 1353 Size: 707 426 19 108 1909 3423 Fragments arranged by size: 3423 1909 707 426 108 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2364 5179 5553 5699 387 Size: 1064 138 775 2815 374 146 1280 Fragments arranged by size: 2815 1280 1064 775 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 3398 4655 5252 6039 6307 1239 Size: 393 409 1357 1257 597 787 268 1524 Fragments arranged by size: 1524 1357 1257 787 597 409 393 268 EcoO109I rG'GnC_Cy Cuts at: 3283 3371 3283 Size: 88 6504 EcoRI G'AATT_C Cuts at: 2846 3001 3616 3901 4279 2846 Size: 155 615 285 378 5159 Fragments arranged by size: 5159 615 378 285 155 EcoRV GAT'ATC Cuts at: 2372 2372 Size: 6592 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 6592 FspI TGC'GCA Cuts at: 5098 5098 Size: 6592 HaeII r_GCGC'y Cuts at: 1678 5739 1678 Size: 4061 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 3806 3838 5233 Size: 32 135 32 645 32 1557 32 1395 Cuts at: 5233 5265 5307 5339 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1557 1395 645 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3936 4182 669 Size: 988 281 1998 246 3079 Fragments arranged by size: 3079 1998 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KpnI G_GTAC'C Cuts at: 3917 3917 Size: 6592 MfeI C'AATT_G Cuts at: 2745 4191 2745 Size: 1446 5146 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3010 5706 5890 1305 Size: 1705 2696 184 2007 Fragments arranged by size: 2696 2007 1705 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 10 Size: 54 1425 343 268 4502 Fragments arranged by size: 4502 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 2344 2646 4550 4909 5068 518 Size: 648 1178 302 1904 359 159 2042 Fragments arranged by size: 2042 1904 1178 648 359 302 159 MspA1I CmG'CkG Cuts at: 1606 2061 4008 4685 5833 6078 1606 Size: 455 1947 677 1148 245 2120 Fragments arranged by size: 2120 1947 1148 677 455 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6040 NdeI CA'TA_TG Cuts at: 387 2830 387 Size: 2443 4149 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 2294 2489 3950 5495 1132 Size: 97 1065 195 1461 1545 2229 Fragments arranged by size: 2229 1545 1461 1065 195 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6592 PciI A'CATG_T Cuts at: 2485 5491 2485 Size: 3006 3586 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6592 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3283 3371 3283 Size: 88 6504 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 2205 4162 2205 Size: 1957 4635 PstI C_TGCA'G Cuts at: 830 2671 3944 830 Size: 1841 1273 3478 Fragments arranged by size: 3478 1841 1273 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 2061 2061 Size: 6592 SalI G'TCGA_C Cuts at: 1936 3934 1936 Size: 1998 4594 SapI GCTCTTCn'nnn_ Cuts at: 2505 2505 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 2579 4840 6552 2579 Size: 2261 1712 2619 Fragments arranged by size: 2619 2261 1712 SfcI C'TryA_G Cuts at: 826 1045 2667 2703 3030 3239 3345 3420 3940 Size: 219 1622 36 327 209 106 75 520 Cuts at: 3940 5075 5756 5947 826 Size: 1135 681 191 1471 Fragments arranged by size: 1622 1471 1135 681 520 327 219 209 191 106 75 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 3919 1872 Size: 2047 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2530 4696 5597 5859 820 Size: 197 926 587 2166 901 262 1553 Fragments arranged by size: 2166 1553 926 901 587 262 197 SnaBI TAC'GTA Cuts at: 493 2875 4383 493 Size: 2382 1508 2702 Fragments arranged by size: 2702 2382 1508 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3950 1132 Size: 2818 3774 SspI AAT'ATT Cuts at: 5 52 2417 3636 3850 4516 5 Size: 47 2365 1219 214 666 2081 Fragments arranged by size: 2365 2081 1219 666 214 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6592 StyI C'CwwG_G Cuts at: 513 1065 2186 2921 4031 513 Size: 552 1121 735 1110 3074 Fragments arranged by size: 3074 1121 1110 735 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3757 4619 4636 4789 4974 5419 325 Size: 1255 2177 862 17 153 185 445 1498 Fragments arranged by size: 2177 1498 1255 862 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 2577 4838 Size: 274 80 33 51 1443 324 276 2261 Cuts at: 4838 6550 96 Size: 1712 138 Fragments arranged by size: 2261 1712 1443 324 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6552 6584 6552 Size: 32 6560 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 3928 3928 Size: 6592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3724 3724 Size: 6592 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 3917 1870 Size: 2047 4545 XmnI GAAnn'nnTTC Cuts at: 2226 2958 3201 3753 4721 2226 Size: 732 243 552 968 4097 Fragments arranged by size: 4097 968 732 552 243 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI