################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaII AviII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglII BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeII MluI MroI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AlwI 836 GGATC GGATC(4/5) AlwNI 151 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1360 CCTCAGG CC^T[ACGT]AGG AseI 883 ATTAAT AT^TAAT AsnI 883 ATTAAT AT^TAAT Asp700I 579 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1088 CTCGAG C^[CT]CG[GA]G AvrII 1047 CCTAGG C^CTAGG BalI 1281 TGGCCA TGG^CCA BbsI 1471 GAAGAC GAAGAC(2/6) BglI 1274 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1047 CCTAGG C^CTAGG BpmI 72 CTGGAG CTGGAG(16/14) BpuAI 1471 GAAGAC GAAGAC(2/6) BsaBI 949 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 435 TCCGGT [AT]^CCGG[AT] BsgI 1220 GTGCAG GTGCAG(16/14) BsmI 814 GAATGC GAATGC(1/-1) BsoBI 1088 CTCGAG C^[CT]CG[GA]G BstYI 836 GGATCT [GA]^GATC[CT] Bsu36I 1360 CCTCAGG CC^T[ACGT]AGG DsaI 418 CCACGG C^C[GA][CT]GG EaeI 1281 TGGCCA [CT]^GGCC[GA] EarI 1508 CTCTTC CTCTTC(1/4) Eco81I 1360 CCTCAGG CC^T[ACGT]AGG HapII 436 CCGG C^CGG HincII 127 GTTAAC GT[CT]^[GA]AC HindII 127 GTTAAC GT[CT]^[GA]AC HpaI 127 GTTAAC GTT^AAC HpaII 436 CCGG C^CGG HphI 636 TCACC GGTGA(8/7) Ksp632I 1508 CTCTTC CTCTTC(1/4) MamI 949 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 849 CAATTG C^AATTG MflI 836 GGATCT [GA]^GATC[CT] MluNI 1281 TGGCCA TGG^CCA MscI 1281 TGGCCA TGG^CCA MslI 1103 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 319 CAGCTG C[AC]G^C[GT]G MspI 436 CCGG C^CGG MunI 849 CAATTG C^AATTG NlaIV 438 GGTTCC GG[ACGT]^[ACGT]CC PaeR7I 1088 CTCGAG C^TCGAG PshBI 883 ATTAAT AT^TAAT PstI 1010 CTGCAG CTGCA^G PvuII 319 CAGCTG CAG^CTG SfcI 1010 CTGCAG C^T[GA][CT]AG SphI 1005 GCATGC GCATG^C StuI 240 AGGCCT AGG^CCT Tsp45I 173 GTGAC ^GT[GC]AC XhoI 1088 CTCGAG C^TCGAG XhoII 836 GGATCT [GA]^GATC[CT] XmnI 579 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BciVI 1118 GGATAC GTATCC(6/5) 1503 GGATAC BsmFI 153 GGGAC GGGAC(10/14) 346 GTCCC Cfr13I 975 GGGCC G^G[ACGT]CC 1076 GGGCC DpnII 837 GATC ^GATC 846 GATC DraI 742 TTTAAA TTT^AAA 933 TTTAAA EcoT14I 588 CCTTGG C^C[AT][AT]GG 1047 CCTAGG MboI 837 GATC ^GATC 846 GATC NdeII 837 GATC ^GATC 846 GATC NspI 990 ACATGC [GA]CATG^[CT] 1005 GCATGC Sau3AI 837 GATC ^GATC 846 GATC Sau96I 975 GGGCC G^G[ACGT]CC 1076 GGGCC SmlI 246 CTCAAG C^T[CT][GA]AG 1088 CTCGAG StyI 588 CCTTGG C^C[AT][AT]GG 1047 CCTAGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII RsaI ScaI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I