(Circular) (Six-base) MAPSORT of: pFN21AE5664.seq Check: 6181 from: 1 to: 6277 pFN21AE5664 6277 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3620 1937 Size: 1683 4594 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4340 5724 5992 1632 Size: 2708 1384 268 1917 Fragments arranged by size: 2708 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 5176 6153 1203 Size: 3973 977 1327 Fragments arranged by size: 3973 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 3112 1166 Size: 1946 4331 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2049 3130 1184 Size: 865 1081 4331 Fragments arranged by size: 4331 1081 865 AlwNI CAG_nnn'CTG Cuts at: 2161 4020 5592 2161 Size: 1859 1572 2846 Fragments arranged by size: 2846 1859 1572 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2271 2407 2744 3586 3812 3964 6073 1493 Size: 778 136 337 842 226 152 2109 1697 Fragments arranged by size: 2109 1697 842 778 337 226 152 136 AseI AT'TA_AT Cuts at: 160 1032 2889 4831 160 Size: 872 1857 1942 1606 Fragments arranged by size: 1942 1857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3093 3602 1870 Size: 73 1150 509 4545 Fragments arranged by size: 4545 1150 509 73 AvrII C'CTAG_G Cuts at: 3052 3052 Size: 6277 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3596 1391 Size: 2205 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3483 928 Size: 971 1584 3722 Fragments arranged by size: 3722 1584 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3117 3502 4169 5379 3117 Size: 385 667 1210 4015 Fragments arranged by size: 4015 1210 667 385 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3285 4888 136 Size: 107 122 71 631 605 1613 1603 1525 Fragments arranged by size: 1613 1603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 4338 5494 1790 Size: 2548 1156 2573 Fragments arranged by size: 2573 2548 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 4008 4966 Size: 416 53 83 399 24 754 1837 958 Cuts at: 4966 442 Size: 1753 Fragments arranged by size: 1837 1753 958 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 4937 1239 Size: 859 2839 2579 Fragments arranged by size: 2839 2579 859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2236 4402 5267 5565 2236 Size: 2166 865 298 2948 Fragments arranged by size: 2948 2166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 2958 6271 2958 Size: 3313 2964 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2440 4710 5108 5382 5529 5940 2440 Size: 2270 398 274 147 411 2777 Fragments arranged by size: 2777 2270 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2071 2101 3134 3164 1178 Size: 30 165 30 668 30 1033 30 4291 Fragments arranged by size: 4291 1033 668 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6277 BseYI C'CCAG_C Cuts at: 5480 5976 5480 Size: 496 5781 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3246 1163 Size: 573 1510 4194 Fragments arranged by size: 4194 1510 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4338 4423 5494 4338 Size: 85 1071 5121 Fragments arranged by size: 5121 1071 85 BsmI GAATG_Cn' Cuts at: 2825 3788 3881 2825 Size: 963 93 5221 Fragments arranged by size: 5221 963 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3596 4338 4423 5494 1391 Size: 399 1806 742 85 1071 2174 Fragments arranged by size: 2174 1806 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5940 5940 Size: 6277 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3632 844 Size: 2788 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2841 3607 3974 4359 4376 5828 5914 5926 Size: 869 766 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 869 766 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3366 3366 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 2423 513 Size: 552 1358 4367 Fragments arranged by size: 4367 1358 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 3580 3793 4587 4607 3580 Size: 213 794 20 5250 Fragments arranged by size: 5250 794 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 2749 2940 3581 2749 Size: 191 641 5445 Fragments arranged by size: 5445 641 191 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 3724 4613 Size: 54 1425 50 275 18 1456 438 889 Cuts at: 4613 6242 8 Size: 1629 43 Fragments arranged by size: 1629 1456 1425 889 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3519 4207 1353 Size: 2166 688 3423 Fragments arranged by size: 3423 2166 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 4340 4937 5724 5992 1239 Size: 393 466 2242 597 787 268 1524 Fragments arranged by size: 2242 1524 787 597 466 393 268 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2403 2819 2403 Size: 416 5861 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4918 4950 4992 5024 1373 Size: 32 135 32 3346 32 42 32 2626 Fragments arranged by size: 3346 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3621 3867 669 Size: 988 281 196 1487 246 3079 Fragments arranged by size: 3079 1487 988 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6277 HpaI GTT'AAC Cuts at: 2134 3867 2134 Size: 1733 4544 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 2854 3876 2854 Size: 1022 5255 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 5391 5575 1305 Size: 1719 2367 184 2007 Fragments arranged by size: 2367 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3288 10 Size: 54 1425 343 1456 2999 Fragments arranged by size: 2999 1456 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3112 4235 4594 4753 518 Size: 648 1946 1123 359 159 2042 Fragments arranged by size: 2042 1946 1123 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2326 3693 4370 5518 5763 1606 Size: 720 1367 677 1148 245 2120 Fragments arranged by size: 2120 1367 1148 720 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5725 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2999 3014 3635 5180 1132 Size: 97 1770 15 621 1545 2229 Fragments arranged by size: 2229 1770 1545 621 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 5176 5176 Size: 6277 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PstI C_TGCA'G Cuts at: 830 3019 3629 830 Size: 2189 610 3478 Fragments arranged by size: 3478 2189 610 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2326 2326 Size: 6277 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 2221 3003 3347 4525 6237 2221 Size: 782 344 1178 1712 2261 Fragments arranged by size: 2261 1712 1178 782 344 SfcI C'TryA_G Cuts at: 826 1045 3015 3625 4760 5441 5632 826 Size: 219 1970 610 1135 681 191 1471 Fragments arranged by size: 1970 1471 1135 681 610 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2251 3093 4381 5282 5544 820 Size: 197 926 308 842 1288 901 262 1553 Fragments arranged by size: 1553 1288 926 901 842 308 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3014 3635 1132 Size: 1882 621 3774 Fragments arranged by size: 3774 1882 621 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 2247 1517 Size: 730 5547 StyI C'CwwG_G Cuts at: 513 1065 2593 3052 3716 513 Size: 552 1528 459 664 3074 Fragments arranged by size: 3074 1528 664 552 459 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4304 4321 4474 4659 5104 325 Size: 1255 2724 17 153 185 445 1498 Fragments arranged by size: 2724 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2125 2219 2294 Size: 274 80 33 51 1443 148 94 75 Cuts at: 2294 2473 3001 3345 4523 6235 96 Size: 179 528 344 1178 1712 138 Fragments arranged by size: 1712 1443 1178 528 344 274 179 148 138 94 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6237 6269 6237 Size: 32 6245 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 3093 1943 Size: 1150 5127 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 2588 4406 2588 Size: 1818 4459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI