Restriction map for
pFN21AE3761
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaBI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 67 GGCGTC G[GA]^CG[CT]C
AvaII 574 GGTCC G^G[AT]CC
BbvCI 1456 CCTCAGC CCTCAGC(-5/-2)
BclI 245 TGATCA T^GATCA
BglII 1438 AGATCT A^GATCT
BsaHI 67 GGCGTC G[GA]^CG[CT]C
BsaI 413 GAGACC GGTCTC(1/5)
BsaWI 1561 ACCGGA [AT]^CCGG[AT]
BsmAI 413 GAGAC GTCTC(1/5)
Bsp1407I 904 TGTACA T^GTACA
BsrGI 904 TGTACA T^GTACA
BstAPI 738 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 940 GGTGACC G^GT[ACGT]ACC
BstPI 940 GGTGACC G^GT[ACGT]ACC
Cfr13I 574 GGTCC G^G[ACGT]CC
DraI 1405 TTTAAA TTT^AAA
DraII 573 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 43 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1607 CCATGG C^C[GA][CT]GG
EcoO109I 573 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 940 GGTGACC G^GT[ACGT]ACC
EcoRV 586 GATATC GAT^ATC
EcoT14I 1607 CCATGG C^C[AT][AT]GG
FauI 27 GCGGG CCCGC(4/6)
FbaI 245 TGATCA T^GATCA
HapII 1562 CCGG C^CGG
HgaI 68 GCGTC GACGC(5/10)
Hin1I 67 GGCGTC G[GA]^CG[CT]C
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
HpaII 1562 CCGG C^CGG
MspA1I 1273 CAGCTG C[AC]G^C[GT]G
MspI 1562 CCGG C^CGG
NcoI 1607 CCATGG C^CATGG
NspI 651 GCATGT [GA]CATG^[CT]
PpuMI 573 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1273 CAGCTG CAG^CTG
SapI 616 GCTCTTC GCTCTTC(1/4)
Sau96I 574 GGTCC G^G[ACGT]CC
SmlI 947 CTCAAG C^T[CT][GA]AG
SpeI 514 ACTAGT A^CTAGT
SspBI 904 TGTACA T^GTACA
StuI 1083 AGGCCT AGG^CCT
StyI 1607 CCATGG C^C[AT][AT]GG
TaqI 411 TCGA T^CGA
TthHB8I 411 TCGA T^CGA
XcmI 1527 CCATTCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 655 GTAC GT^AC
905 GTAC
AlwI 675 GATCC GGATC(4/5)
523 GGATC
BanI 73 GGCACC G^G[CT][GA]CC
628 GGCACC
BbsI 835 GAAGAC GAAGAC(2/6)
1575 GTCTTC
BfaI 515 CTAG C^TAG
1031 CTAG
BpmI 290 CTGGAG CTGGAG(16/14)
591 CTGGAG
BpuAI 835 GAAGAC GAAGAC(2/6)
1575 GTCTTC
BsaAI 46 TACGTG [CT]AC^GT[GA]
1348 TACGTG
BsmI 453 GAATGC GAATGC(1/-1)
1299 GAATGC
BspMI 571 GCAGGT ACCTGC(4/8)
747 GCAGGT
Cac8I 957 GCTTGC GC[ACGT]^[ACGT]GC
1477 GCTGGC
EarI 617 CTCTTC CTCTTC(1/4)
334 GAAGAG
HaeIII 115 GGCC GG^CC
1084 GGCC
Ksp632I 617 CTCTTC CTCTTC(1/4)
334 GAAGAG
MaeI 515 CTAG C^TAG
1031 CTAG
MslI 448 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1309 CATCTTCGTG
NdeI 397 CATATG CA^TATG
739 CATATG
PflMI 117 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1333 CCAGTGACTGG
PstI 745 CTGCAG CTGCA^G
1517 CTGCAG
RsaI 655 GTAC GT^AC
905 GTAC
SexAI 239 ACCAGGT A^CC[AT]GGT
1373 ACCTGGT
SfcI 745 CTGCAG C^T[GA][CT]AG
1517 CTGCAG
Van91I 117 CCATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1333 CCAGTGACTGG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI AlwNI ApoI
BbvI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI BstYI DdeI
DpnII EcoRII EcoT22I Fnu4HI FokI
HinfI HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NsiI Sau3AI ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII