(Linear) (Six-base) MAPSORT of: KIEE6275.seq Check: 4209 from: 1 to: 615 KIEE6275.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 615 Size: 571 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 24 615 Size: 24 591 BglI GCCn_nnn'nGGC Cuts at: 0 46 615 Size: 46 569 BglII A'GATC_T Cuts at: 0 539 615 Size: 539 76 BlpI GC'TnA_GC Cuts at: 0 344 615 Size: 344 271 BmtI G_CTAG'C Cuts at: 0 13 615 Size: 13 602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 579 615 Size: 579 36 BsaBI GATnn'nnATC Cuts at: 0 529 615 Size: 529 86 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 446 476 615 Size: 446 30 139 Fragments arranged by size: 446 139 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 210 251 615 Size: 210 41 364 Fragments arranged by size: 364 210 41 BseYI C'CCAG_C Cuts at: 0 508 615 Size: 508 107 BsrFI r'CCGG_y Cuts at: 0 214 615 Size: 214 401 BssHII G'CGCG_C Cuts at: 0 53 615 Size: 53 562 BstXI CCAn_nnnn'nTGG Cuts at: 0 270 615 Size: 270 345 BstYI r'GATC_y Cuts at: 0 539 615 Size: 539 76 BtgI C'CryG_G Cuts at: 0 62 615 Size: 62 553 BtsI GCAGTG_nn' Cuts at: 0 13 483 615 Size: 13 470 132 Fragments arranged by size: 470 132 13 EaeI y'GGCC_r Cuts at: 0 59 299 378 491 615 Size: 59 240 79 113 124 Fragments arranged by size: 240 124 113 79 59 EarI CTCTTCn'nnn_ Cuts at: 0 451 615 Size: 451 164 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 579 615 Size: 571 8 36 Fragments arranged by size: 571 36 8 EcoO109I rG'GnC_Cy Cuts at: 0 246 615 Size: 246 369 HaeII r_GCGC'y Cuts at: 0 554 615 Size: 554 61 MfeI C'AATT_G Cuts at: 0 323 615 Size: 323 292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 173 615 Size: 173 442 MscI TGG'CCA Cuts at: 0 301 615 Size: 301 314 MslI CAynn'nnrTG Cuts at: 0 316 615 Size: 316 299 NheI G'CTAG_C Cuts at: 0 9 615 Size: 9 606 SexAI A'CCwGG_T Cuts at: 0 269 615 Size: 269 346 SfiI GGCCn_nnn'nGGCC Cuts at: 0 46 615 Size: 46 569 StuI AGG'CCT Cuts at: 0 434 615 Size: 434 181 StyI C'CwwG_G Cuts at: 0 368 615 Size: 368 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 598 615 Size: 598 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 446 478 615 Size: 446 32 137 Fragments arranged by size: 446 137 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 87 603 615 Size: 87 516 12 Fragments arranged by size: 516 87 12 Enzymes that do cut: AcuI BbsI BglI BglII BlpI BmtI BpmI BsaBI BsaXI BseRI BseYI BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I HaeII MfeI MmeI MscI MslI NheI SexAI SfiI StuI StyI TaqII TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI