################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbrPI BbvCI BcgI BciVI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MfeI MluI MroI MunI MvnI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AflII 144 CTTAAG C^TTAAG AlwNI 73 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 465 GAGCTC G[AT]GC[AT]^C AvaI 62 CTCGGG C^[CT]CG[GA]G BalI 747 TGGCCA TGG^CCA BamHI 303 GGATCC G^GATCC BanI 90 GGCGCC G^G[CT][GA]CC BanII 465 GAGCTC G[GA]GC[CT]^C BbeI 90 GGCGCC GGCGC^C BbsI 219 GAAGAC GAAGAC(2/6) BclI 398 TGATCA T^GATCA BcnI 10 CCCGG CC^[GC]GG BfaI 838 CTAG C^TAG BfrI 144 CTTAAG C^TTAAG BmyI 465 GAGCTC G[AGT]GC[ACT]^C BpuAI 219 GAAGAC GAAGAC(2/6) BsaI 778 GAGACC GGTCTC(1/5) BsiHKAI 465 GAGCTC G[AT]GC[AT]^C BsmI 501 GAATGC GAATGC(1/-1) BsoBI 62 CTCGGG C^[CT]CG[GA]G Bsp1286I 465 GAGCTC G[AGT]GC[ACT]^C Bsp1407I 788 TGTACA T^GTACA BspMI 52 ACCTGC ACCTGC(4/8) BsrBI 622 GAGCGG CCGCTC(-3/-3) BsrFI 87 GCCGGC [GA]^CCGG[CT] BsrGI 788 TGTACA T^GTACA BsrI 554 ACTGG ACTGG(1/-1) BssSI 538 CTCGTG CACGAG(-5/-1) BstYI 303 GGATCC [GA]^GATC[CT] Cfr10I 87 GCCGGC [GA]^CCGG[CT] DsaI 46 CCATGG C^C[GA][CT]GG EaeI 747 TGGCCA [CT]^GGCC[GA] EcoNI 225 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 398 TGATCA T^GATCA HgaI 4 GCGTC GACGC(5/10) KasI 90 GGCGCC G^GCGCC MaeI 838 CTAG C^TAG MflI 303 GGATCC [GA]^GATC[CT] MluNI 747 TGGCCA TGG^CCA MscI 747 TGGCCA TGG^CCA NaeI 87 GCCGGC GCC^GGC NarI 90 GGCGCC GG^CGCC NciI 10 CCCGG CC^[GC]GG NcoI 46 CCATGG C^CATGG NgoMIV 87 GCCGGC G^CCGGC PflMI 907 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 70 CTGCAG CTGCA^G PvuII 129 CAGCTG CAG^CTG SacI 465 GAGCTC GAGCT^C SfoI 90 GGCGCC GGC^GCC SgrAI 86 CGCCGGCG C[GA]^CCGG[CT]G SmlI 144 CTTAAG C^T[CT][GA]AG SspBI 788 TGTACA T^GTACA TaqI 450 TCGA T^CGA Tsp45I 479 GTGAC ^GT[GC]AC TthHB8I 450 TCGA T^CGA Van91I 907 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 303 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 191 GAATTT [GA]^AATT[CT] 685 AAATTC AcyI 3 GGCGTC G[GA]^CG[CT]C 90 GGCGCC AfaI 513 GTAC GT^AC 789 GTAC AlwI 304 GATCC GGATC(4/5) 303 GGATC ApoI 191 GAATTT [GA]^AATT[CT] 685 AAATTC Asp700I 243 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 760 GAAACCATTC BsaBI 577 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 610 GATATAAATC BsaHI 3 GGCGTC G[GA]^CG[CT]C 90 GGCGCC BsmAI 125 GAGAC GTCTC(1/5) 778 GAGAC BsmFI 420 GGGAC GGGAC(10/14) 860 GGGAC Cac8I 87 GCCGGC GC[ACGT]^[ACGT]GC 543 GCAAGC FauI 84 CCCGC CCCGC(4/6) 624 GCGGG HaeII 66 GGCGCT [GA]GCGC^[CT] 90 GGCGCC HapII 11 CCGG C^CGG 88 CCGG Hin1I 3 GGCGTC G[GA]^CG[CT]C 90 GGCGCC HpaII 11 CCGG C^CGG 88 CCGG MaeII 197 ACGT A^CGT 236 ACGT MaeIII 274 GTAAC ^GT[ACGT]AC 479 GTGAC MamI 577 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 610 GATATAAATC MseI 145 TTAA T^TAA 161 TTAA MslI 427 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 472 CACATCTGTG MspA1I 94 CCGCTG C[AC]G^C[GT]G 129 CAGCTG MspI 11 CCGG C^CGG 88 CCGG PleI 443 GACTC GAGTC(4/5) 637 GAGTC RsaI 513 GTAC GT^AC 789 GTAC Tru9I 145 TTAA T^TAA 161 TTAA XmnI 243 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 760 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp509I TspRI