################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsmI Bsp1407I BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI CelII CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1668 GTATAC GT^[AC][GT]AC AccIII 164 TCCGGA T^CCGGA AcsI 61 GAATTT [GA]^AATT[CT] AcyI 217 GGCGTC G[GA]^CG[CT]C AflIII 642 ACGCGT A^C[GA][CT]GT AhdI 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 346 CCTAAGG CC^T[ACGT]AGG ApoI 61 GAATTT [GA]^AATT[CT] Asp700I 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 460 GTCTTC GAAGAC(2/6) BcgI 1205 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 460 GTCTTC GAAGAC(2/6) BsaHI 217 GGCGTC G[GA]^CG[CT]C BsaI 405 GAGACC GGTCTC(1/5) BseAI 164 TCCGGA T^CCGGA BsiWI 1286 CGTACG C^GTACG BsmBI 1290 CGTCTC CGTCTC(1/5) BspDI 418 ATCGAT AT^CGAT BspEI 164 TCCGGA T^CCGGA Bst1107I 1668 GTATAC GTA^TAC BstAPI 588 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1668 GTATAC GTA^TAC Bsu36I 346 CCTAAGG CC^T[ACGT]AGG ClaI 418 ATCGAT AT^CGAT DraIII 1699 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 407 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 379 GAAGAG CTCTTC(1/4) Eco81I 346 CCTAAGG CC^T[ACGT]AGG Hin1I 217 GGCGTC G[GA]^CG[CT]C Ksp632I 379 GAAGAG CTCTTC(1/4) KspI 328 CCGCGG CCGC^GG MaeIII 740 GTGAC ^GT[ACGT]AC MluI 642 ACGCGT A^CGCGT MroI 164 TCCGGA T^CCGGA PaeR7I 1532 CTCGAG C^TCGAG PpuMI 1162 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 1750 CAGCTG CAG^CTG SacII 328 CCGCGG CCGC^GG ScaI 1538 AGTACT AGT^ACT SexAI 1700 ACCTGGT A^CC[AT]GGT SmlI 1532 CTCGAG C^T[CT][GA]AG StuI 125 AGGCCT AGG^CCT Tsp45I 740 GTGAC ^GT[GC]AC Tsp509I 62 AATT ^AATT XcmI 233 CCAACACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1532 CTCGAG C^TCGAG XmnI 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Aor51HI 770 AGCGCT AGC^GCT 989 AGCGCT BanI 88 GGCACC G^G[CT][GA]CC 340 GGCACC BbvCI 310 CCTCAGC CCTCAGC(-5/-2) 118 GCTGAGG BfaI 322 CTAG C^TAG 1220 CTAG BglI 80 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1622 GCCCGTGGGGC BsaWI 164 TCCGGA [AT]^CCGG[AT] 297 ACCGGA BsgI 1119 GTGCAG GTGCAG(16/14) 1597 GTGCAG BsmAI 405 GAGAC GTCTC(1/5) 1291 GTCTC BsrFI 845 GCCGGC [GA]^CCGG[CT] 1064 GCCGGC Cfr10I 845 GCCGGC [GA]^CCGG[CT] 1064 GCCGGC Eco47III 770 AGCGCT AGC^GCT 989 AGCGCT MaeI 322 CTAG C^TAG 1220 CTAG NaeI 845 GCCGGC GCC^GGC 1064 GCCGGC NcoI 444 CCATGG C^CATGG 1469 CCATGG NgoMIV 845 GCCGGC G^CCGGC 1064 GCCGGC PstI 142 CTGCAG CTGCA^G 205 CTGCAG SacI 1006 GAGCTC GAGCT^C 1429 GAGCTC SfaNI 476 GCATC GCATC(5/9) 1153 GCATC TfiI 680 GATTC G^A[AT]TC 956 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TseI TspRI TthHB8I Van91I XhoII