(Circular) (Six-base) MAPSORT of: pFN21AB7978.seq Check: 1393 from: 1 to: 5992 pFN21AB7978 5992 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3802 278 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2704 4055 5439 5707 1632 Size: 1072 1351 1384 268 1917 Fragments arranged by size: 1917 1384 1351 1072 268 AfeI AGC'GCT Cuts at: 3148 3148 Size: 5992 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 4891 5868 1203 Size: 3688 977 1327 Fragments arranged by size: 3688 1327 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 4721 4721 Size: 5992 AleI CACnn'nnGTG Cuts at: 1166 2531 1166 Size: 1365 4627 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2756 2788 2756 Size: 32 5960 AlwNI CAG_nnn'CTG Cuts at: 2605 3735 5307 2605 Size: 1130 1572 3290 Fragments arranged by size: 3290 1572 1130 ApaI G_GGCC'C Cuts at: 2178 2992 3012 2178 Size: 814 20 5158 Fragments arranged by size: 5158 814 20 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AscI GG'CGCG_CC Cuts at: 2185 2185 Size: 5992 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2195 2577 2691 2858 2899 2983 3317 Size: 73 252 382 114 167 41 84 334 Cuts at: 3317 1870 Size: 4545 Fragments arranged by size: 4545 382 334 252 167 114 84 73 41 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2349 2382 2349 Size: 33 5959 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2592 2750 3313 4768 618 Size: 325 217 627 805 158 563 1455 1842 Fragments arranged by size: 1842 1455 805 627 563 325 217 158 BanII G_rGCy'C Cuts at: 1391 2178 2244 2586 2992 3012 3311 1391 Size: 787 66 342 406 20 299 4072 Fragments arranged by size: 4072 787 406 342 299 66 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5021 BbvCI CC'TCA_GC Cuts at: 2921 2921 Size: 5992 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BfrBI ATG'CAT Cuts at: 2851 2851 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2182 2590 2694 Size: 107 122 71 631 605 510 408 104 Cuts at: 2694 3192 4603 136 Size: 498 1411 1525 Fragments arranged by size: 1525 1411 631 605 510 498 408 122 107 104 71 BglII A'GATC_T Cuts at: 5987 5987 Size: 5992 BlpI GC'TnA_GC Cuts at: 2153 2403 3409 2153 Size: 250 1006 4736 Fragments arranged by size: 4736 1006 250 Bme1580I G_kGCm'C Cuts at: 1790 2178 2906 2992 3012 4053 5209 1790 Size: 388 728 86 20 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 728 388 86 20 BmgBI CAC'GTC Cuts at: 1318 2218 2347 1318 Size: 900 129 4963 Fragments arranged by size: 4963 900 129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3723 4681 442 Size: 416 53 83 399 24 2306 958 1753 Fragments arranged by size: 2306 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2413 2717 2221 Size: 192 304 5496 Fragments arranged by size: 5496 304 192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 4652 1239 Size: 1388 2025 2579 Fragments arranged by size: 2579 2025 1388 Bpu10I CC'TnA_GC Cuts at: 2921 3248 2921 Size: 327 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4655 882 Size: 617 3156 2219 Fragments arranged by size: 3156 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3783 493 Size: 711 2579 2702 Fragments arranged by size: 2702 2579 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3799 4181 4816 275 Size: 53 83 186 936 2266 382 635 1451 Fragments arranged by size: 2266 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 3059 3089 1178 Size: 30 165 30 1353 30 273 30 4081 Fragments arranged by size: 4081 1353 273 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5992 BseYI C'CCAG_C Cuts at: 2684 3182 5195 5691 2684 Size: 498 2013 496 2985 Fragments arranged by size: 2985 2013 498 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2169 2424 3109 3442 4203 5231 Size: 275 186 168 255 685 333 761 1028 Cuts at: 5231 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 685 333 275 255 186 168 BsiHKAI G_wGCw'C Cuts at: 2244 2681 3254 4053 4138 5209 2244 Size: 437 573 799 85 1071 3027 Fragments arranged by size: 3027 1071 799 573 437 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2244 2586 2681 2906 2992 3012 Size: 399 388 66 342 95 225 86 20 Cuts at: 3012 3254 3311 4053 4138 5209 1391 Size: 242 57 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 342 242 225 95 86 85 66 57 20 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 2427 3174 3881 5611 2427 Size: 747 707 1730 2808 Fragments arranged by size: 2808 1730 747 707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3347 844 Size: 2503 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2796 3006 3111 3879 1394 Size: 429 973 210 105 768 3507 Fragments arranged by size: 3507 973 768 429 210 105 BsrDI GCAATG_nn' Cuts at: 65 1248 2389 2419 4487 4661 65 Size: 1183 1141 30 2068 174 1396 Fragments arranged by size: 2068 1396 1183 1141 174 30 BsrFI r'CCGG_y Cuts at: 1700 1810 2595 2938 3086 3164 4636 4823 1700 Size: 110 785 343 148 78 1472 187 2869 Fragments arranged by size: 2869 1472 785 343 187 148 110 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 2148 2157 2185 1925 Size: 223 9 28 5732 Fragments arranged by size: 5732 223 28 9 BssSI C'ACGA_G Cuts at: 2243 2905 4052 5064 2243 Size: 662 1147 1012 3171 Fragments arranged by size: 3171 1147 1012 662 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5992 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 2560 1184 Size: 1376 4616 BstXI CCAn_nnnn'nTGG Cuts at: 2533 3203 2533 Size: 670 5322 BstYI r'GATC_y Cuts at: 1972 3322 3689 4074 4091 5543 5629 5641 5987 Size: 1350 367 385 17 1452 86 12 346 Cuts at: 5987 1972 Size: 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2565 3078 3240 513 Size: 552 939 165 396 513 162 3265 Fragments arranged by size: 3265 939 552 513 396 165 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5253 Fragments arranged by size: 5253 683 56 BtsI GCAGTG_nn' Cuts at: 2482 3103 3508 4302 4322 2482 Size: 621 405 794 20 4152 Fragments arranged by size: 4152 794 621 405 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 2421 2442 Size: 54 1425 50 275 18 336 255 21 Cuts at: 2442 2940 3084 3193 3439 4328 5957 8 Size: 498 144 109 246 889 1629 43 Fragments arranged by size: 1629 1425 889 498 336 275 255 246 144 109 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2166 2421 3439 1537 Size: 275 354 255 1018 4090 Fragments arranged by size: 4090 1018 354 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 3922 1353 Size: 1130 1439 3423 Fragments arranged by size: 3423 1439 1130 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2240 4579 4953 5099 387 Size: 1064 138 651 2339 374 146 1280 Fragments arranged by size: 2339 1280 1064 651 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2242 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 2704 4055 4652 5439 5707 1239 Size: 393 995 77 1351 597 787 268 1524 Fragments arranged by size: 1524 1351 995 787 597 393 268 77 EcoO109I rG'GnC_Cy Cuts at: 2174 2644 2988 2989 3009 2174 Size: 470 344 1 20 5157 Fragments arranged by size: 5157 470 344 20 1 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FspI TGC'GCA Cuts at: 2249 2411 2660 4498 2249 Size: 162 249 1838 3743 Fragments arranged by size: 3743 1838 249 162 FspAI rTGC'GCAy Cuts at: 2249 2249 Size: 5992 HaeII r_GCGC'y Cuts at: 1678 2299 2892 3150 5139 1678 Size: 621 593 258 1989 2531 Fragments arranged by size: 2531 1989 621 593 258 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 4633 4665 4707 Size: 32 135 32 1267 32 1762 32 42 Cuts at: 4707 4739 1373 Size: 32 2626 Fragments arranged by size: 2626 1762 1267 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2799 5106 5290 1305 Size: 1494 2307 184 2007 Fragments arranged by size: 2307 2007 1494 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 10 Size: 54 1425 343 612 3558 Fragments arranged by size: 3558 1425 612 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2510 2531 2558 2666 3950 4309 4468 Size: 648 1344 21 27 108 1284 359 159 Cuts at: 4468 518 Size: 2042 Fragments arranged by size: 2042 1344 1284 648 359 159 108 27 21 MspA1I CmG'CkG Cuts at: 1606 2145 2402 2873 3408 4085 5233 5478 1606 Size: 539 257 471 535 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 539 535 471 257 245 NaeI GCC'GGC Cuts at: 2940 3088 3166 2940 Size: 148 78 5766 Fragments arranged by size: 5766 148 78 NcoI C'CATG_G Cuts at: 513 1065 2004 3078 513 Size: 552 939 1074 3427 Fragments arranged by size: 3427 1074 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 2938 3086 3164 2938 Size: 148 78 5766 Fragments arranged by size: 5766 148 78 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NsiI A_TGCA'T Cuts at: 2853 2853 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2665 2938 3127 3350 4895 1132 Size: 97 1436 273 189 223 1545 2229 Fragments arranged by size: 2229 1545 1436 273 223 189 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 4891 4891 Size: 5992 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2756 2788 4061 4093 5574 5606 2756 Size: 32 1273 32 1481 32 3142 Fragments arranged by size: 3142 1481 1273 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2644 2644 Size: 5992 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PspOMI G'GGCC_C Cuts at: 2174 2988 3008 2174 Size: 814 20 5158 Fragments arranged by size: 5158 814 20 PstI C_TGCA'G Cuts at: 830 3344 830 Size: 2514 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5992 PvuII CAG'CTG Cuts at: 2873 2873 Size: 5992 SacI G_AGCT'C Cuts at: 2244 2244 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SbfI CC_TGCA'GG Cuts at: 3344 3344 Size: 5992 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 2884 3340 4475 5156 5347 826 Size: 219 1839 456 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 456 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 2579 2860 2985 3319 1872 Size: 707 281 125 334 4545 Fragments arranged by size: 4545 707 334 281 125 SmlI C'TyrA_G Cuts at: 820 1017 1943 4096 4997 5259 820 Size: 197 926 2153 901 262 1553 Fragments arranged by size: 2153 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 2665 2938 3127 3350 1132 Size: 1533 273 189 223 3774 Fragments arranged by size: 3774 1533 273 223 189 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5992 StyI C'CwwG_G Cuts at: 513 1065 2004 2746 3078 3431 513 Size: 552 939 742 332 353 3074 Fragments arranged by size: 3074 939 742 552 353 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2543 2698 3253 4019 4036 4189 4374 Size: 1255 963 155 555 766 17 153 185 Cuts at: 4374 4819 325 Size: 445 1498 Fragments arranged by size: 1498 1255 963 766 555 445 185 155 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4238 5950 96 Size: 274 80 33 51 1443 2261 1712 138 Fragments arranged by size: 2261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5952 5984 5952 Size: 32 5960 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5992 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5992 XmaI C'CCGG_G Cuts at: 1870 2577 2858 2983 3317 1870 Size: 707 281 125 334 4545 Fragments arranged by size: 4545 707 334 281 125 XmnI GAAnn'nnTTC Cuts at: 2492 2768 4121 2492 Size: 276 1353 4363 Fragments arranged by size: 4363 1353 276 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3800 276 Size: 53 83 186 936 2266 2468 Fragments arranged by size: 2468 2266 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AvrII BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI