Restriction map for
pFN21AE1994
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsiEI BsmI BsoBI Bsp1407I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 154 GTCTAC GT^[AC][GT]AC
AcsI 85 AAATTC [GA]^AATT[CT]
AflIII 644 ACATGT A^C[GA][CT]GT
ApaI 1078 GGGCCC GGGCC^C
ApoI 85 AAATTC [GA]^AATT[CT]
AspI 148 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 450 TGGCCA TGG^CCA
BamHI 31 GGATCC G^GATCC
BbrPI 79 CACGTG CAC^GTG
BbvCI 864 CCTCAGC CCTCAGC(-5/-2)
BcnI 224 CCCGG CC^[GC]GG
BfaI 1259 CTAG C^TAG
BglI 1132 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 79 CACGTG [CT]AC^GT[GA]
BsaI 1645 GAGACC GGTCTC(1/5)
BsiWI 176 CGTACG C^GTACG
BsmBI 250 GAGACG CGTCTC(1/5)
BspDI 399 ATCGAT AT^CGAT
BspHI 602 TCATGA T^CATGA
BspLU11I 644 ACATGT A^CATGT
BsrDI 1388 CATTGC GCAATG(2/0)
CfoI 825 GCGC GCG^C
ClaI 399 ATCGAT AT^CGAT
DsaI 713 CCATGG C^C[GA][CT]GG
EaeI 450 TGGCCA [CT]^GGCC[GA]
EcoT22I 1215 ATGCAT ATGCA^T
HgaI 6 GACGC GACGC(5/10)
HhaI 825 GCGC GCG^C
HinP1I 825 GCGC G^CGC
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
MaeI 1259 CTAG C^TAG
MluNI 450 TGGCCA TGG^CCA
MscI 450 TGGCCA TGG^CCA
NciI 224 CCCGG CC^[GC]GG
NcoI 713 CCATGG C^CATGG
NsiI 1215 ATGCAT ATGCA^T
PflFI 148 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
PpuMI 56 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1570 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 602 TCATGA T^CATGA
SbfI 934 CCTGCAGG CCTGCA^GG
SfaNI 161 GCATC GCATC(5/9)
Sse8387I 934 CCTGCAGG CCTGCA^GG
Tth111I 148 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 694 TCCGGA T^CCGGA
1185 TCCGGA
BanI 139 GGCACC G^G[CT][GA]CC
595 GGCACC
BbsI 469 GAAGAC GAAGAC(2/6)
657 GAAGAC
BpuAI 469 GAAGAC GAAGAC(2/6)
657 GAAGAC
BseAI 694 TCCGGA T^CCGGA
1185 TCCGGA
BseRI 1492 CTCCTC GAGGAG(10/8)
1497 CTCCTC
BsmAI 250 GAGAC GTCTC(1/5)
1645 GAGAC
BspEI 694 TCCGGA T^CCGGA
1185 TCCGGA
BsrFI 308 GCCGGT [GA]^CCGG[CT]
455 ACCGGC
Cfr10I 308 GCCGGT [GA]^CCGG[CT]
455 ACCGGC
FauI 128 GCGGG CCCGC(4/6)
301 GCGGG
MroI 694 TCCGGA T^CCGGA
1185 TCCGGA
MslI 318 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
597 CACCATCATG
NspI 644 ACATGT [GA]CATG^[CT]
1319 GCATGT
PflMI 1303 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGCCCCTGG
PvuII 1191 CAGCTG CAG^CTG
1307 CAGCTG
ScaI 732 AGTACT AGT^ACT
899 AGTACT
SmlI 361 CTCAAG C^T[CT][GA]AG
1175 CTCAAG
StuI 1276 AGGCCT AGG^CCT
1692 AGGCCT
Tsp509I 86 AATT ^AATT
1118 AATT
Van91I 1303 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1395 CCAGCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BanII BbvI
BmyI BpmI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII Eco81I
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI XhoII