################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MslI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 176 TCCGGA T^CCGGA AluI 623 AGCT AG^CT AlwNI 490 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG BalI 38 TGGCCA TGG^CCA BanI 301 GGCACC G^G[CT][GA]CC BcgI 448 CGATTCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaI 203 GAGACC GGTCTC(1/5) BsaWI 176 TCCGGA [AT]^CCGG[AT] BseAI 176 TCCGGA T^CCGGA BsgI 456 CTGCAC GTGCAG(16/14) BsiEI 444 CGGCCG CG[GA][CT]^CG BsmBI 525 CGTCTC CGTCTC(1/5) BspEI 176 TCCGGA T^CCGGA BsrI 501 CCAGT ACTGG(1/-1) BstXI 41 CCATATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 689 CTGAG C^T[ACGT]AG DsaI 279 CCGTGG C^C[GA][CT]GG EagI 444 CGGCCG C^GGCCG EarI 589 CTCTTC CTCTTC(1/4) EclXI 444 CGGCCG C^GGCCG Eco52I 444 CGGCCG C^GGCCG EcoNI 530 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HinfI 449 GATTC G^A[ACGT]TC Ksp632I 589 CTCTTC CTCTTC(1/4) MaeII 386 ACGT A^CGT MluNI 38 TGGCCA TGG^CCA MroI 176 TCCGGA T^CCGGA MscI 38 TGGCCA TGG^CCA NlaIII 612 CATG CATG^ PpuMI 6 GGGTCCC [GA]G^G[AT]CC[CT] PstI 625 CTGCAG CTGCA^G PvuII 622 CAGCTG CAG^CTG SapI 588 GCTCTTC GCTCTTC(1/4) SexAI 638 ACCAGGT A^CC[AT]GGT SfcI 625 CTGCAG C^T[GA][CT]AG TaqI 398 TCGA T^CGA TfiI 449 GATTC G^A[AT]TC Tsp509I 294 AATT ^AATT TthHB8I 398 TCGA T^CGA XcmI 207 CCAGGGGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 429 GTAC GT^AC 463 GTAC AspHI 25 GTGCTC G[AT]GC[AT]^C 80 GAGCAC AvaI 11 CCCGGG C^[CT]CG[GA]G 288 CCCGGG BbsI 110 GAAGAC GAAGAC(2/6) 703 GTCTTC BpuAI 110 GAAGAC GAAGAC(2/6) 703 GTCTTC BseRI 528 CTCCTC GAGGAG(10/8) 531 CTCCTC BsiHKAI 25 GTGCTC G[AT]GC[AT]^C 80 GAGCAC BsmAI 203 GAGAC GTCTC(1/5) 526 GTCTC BsmFI 8 GTCCC GGGAC(10/14) 498 GTCCC BsoBI 11 CCCGGG C^[CT]CG[GA]G 288 CCCGGG BstEII 60 GGTGACC G^GT[ACGT]ACC 634 GGTGACC BstPI 60 GGTGACC G^GT[ACGT]ACC 634 GGTGACC EcoO65I 60 GGTGACC G^GT[ACGT]ACC 634 GGTGACC EcoT14I 189 CCAAGG C^C[AT][AT]GG 305 CCAAGG HgaI 551 GACGC GACGC(5/10) 701 GCGTC MseI 197 TTAA T^TAA 296 TTAA RsaI 429 GTAC GT^AC 463 GTAC SmaI 11 CCCGGG CCC^GGG 288 CCCGGG SmlI 139 CTCAAG C^T[CT][GA]AG 534 CTCAAG StuI 135 AGGCCT AGG^CCT 192 AGGCCT StyI 189 CCAAGG C^C[AT][AT]GG 305 CCAAGG Tru9I 197 TTAA T^TAA 296 TTAA TspRI 459 CACTG CA[GC]TG[ACGT][ACGT]^ 502 CAGTG XmaCI 11 CCCGGG C^CCGGG 288 CCCGGG XmaI 11 CCCGGG C^CCGGG 288 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI ApaI AspI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PflFI Sau96I ScrFI SfaNI TseI Tsp45I Tth111I